Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: fruiting bodies
The structure was elucidated in this paperPublication DOI: 10.1016/j.ijbiomac.2022.01.101Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Y. Yang <yangyanjing
csu.edu.cn>; S. Zhong <zhongshian
aliyun.com>
Institutions: College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China
The development and application of new natural selenium polysaccharides with relatively clear structure and excellent activity have become hot and difficult issues. This study used GC–MS and 2D NMR to characterize the detailed chain structure information of selenium polysaccharide (Se-POP-3) from Selenium-enriched Pleurotus ostreatus, and then explored its anti-gastric cancer and anti-colon cancer effects in vitro. Results showed that the main chain of Se-POP-3 was →[3)-β-D-Glcp-(1]2→6)-β-D-Glcp-(1→3,6)-β-D-Glcp-(1→3)-β-D-Glcp-(1→, and the branch was α-D-Glcp-(1 →[4)-α-D-Glcp-(1]4→, which was connected to the main chain through the O-3 bond of →3,6)-β-D-Glcp-(1→ glycosidic bond. In addition, Se-POP-3 could reduce viability, induce apoptosis, inhibit migration and invasion, destroy the Bax/Bcl-2 ratio, and inhibit the epithelial-to-mesenchymal transition of MGC-803 and HCT-116 cells in vitro. Moreover, this study also showed that within the concentration range set in this study, Se-POP-3 had no significant effect on the growth of normal cells (NCM460 cells). This study can provide a theoretical basis for the potential application of Se-POP-3 as an anti-gastrointestinal cancer drug or functional food.
antitumor activity, Pleurotus ostreatus, selenium-enriched polysaccharide
Structure type: structural motif or average structure
Location inside paper: p. 634, Fig. 3, table 2, Se-POP-3
Trivial name: selenium polysaccharide (Se-POP-3)
Contained glycoepitopes: IEDB_135614,IEDB_1397514,IEDB_140629,IEDB_141806,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_153543,IEDB_153755,IEDB_158555,IEDB_241101,IEDB_420417,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GC-MS, Western blotting, extraction, statistical analysis, cell viability assay, cytotoxicity assay, ion exchange chromatography, staining, annexin V–FITC/PI assay, wound healing assay, transwell assay
Comments, role: Se-POP-3 was purified from selenium-enriched Pleurotus ostreatus; the main chain of Se-POP-3 polysaccharide; published erroneous NMR chemical shifts of C3 #3_bDGlcp (73.11) was removed by CSDB staff
NCBI Taxonomy refs (TaxIDs): 5322
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,4 aDGlcp 99.73 71.50 73.23 76.73 70.38 60.44
3,6,6,3 bDGlcp 102.17 72.85 84.26 69.46 75.89 60.69
3,6,6 bDGlcp
3,6 bDGlcp 102.97 73.03 75.56 69.29 74.88 68.79
3,3,4,4 aDGlcp 98.12 71.14 73.44 71.71 69.57 60.98
3,3 aDGlcp
3 bDGlcp 102.68 74.30 ? 69.46 75.89 68.79
bDGlcp 102.17 72.85 84.26 69.46 75.89 60.69
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,4 aDGlcp 5.31 3.55 3.89 3.59 3.76 3.75-3.86
3,6,6,3 bDGlcp 4.72 3.43 3.65 3.41 3.43 3.68-3.84
3,6,6 bDGlcp
3,6 bDGlcp 4.44 3.23 3.39 3.30 3.55 3.77-4.15
3,3,4,4 aDGlcp 4.90 3.76 3.84 3.54 3.93 3.62-3.78
3,3 aDGlcp
3 bDGlcp 4.68 3.28 3.57 3.41 3.43 3.77-4.15
bDGlcp 4.72 3.43 3.65 3.41 3.43 3.68-3.84
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,4 aDGlcp 99.73/5.31 71.50/3.55 73.23/3.89 76.73/3.59 70.38/3.76 60.44/3.75-3.86
3,6,6,3 bDGlcp 102.17/4.72 72.85/3.43 84.26/3.65 69.46/3.41 75.89/3.43 60.69/3.68-3.84
3,6,6 bDGlcp
3,6 bDGlcp 102.97/4.44 73.03/3.23 75.56/3.39 69.29/3.30 74.88/3.55 68.79/3.77-4.15
3,3,4,4 aDGlcp 98.12/4.90 71.14/3.76 73.44/3.84 71.71/3.54 69.57/3.93 60.98/3.62-3.78
3,3 aDGlcp
3 bDGlcp 102.68/4.68 74.30/3.28 ?/3.57 69.46/3.41 75.89/3.43 68.79/3.77-4.15
bDGlcp 102.17/4.72 72.85/3.43 84.26/3.65 69.46/3.41 75.89/3.43 60.69/3.68-3.84
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,4 | aDGlcp | 5.31 | 3.55 | 3.89 | 3.59 | 3.76 | 3.75
3.86 |
| 3,6,6,3 | bDGlcp | 4.72 | 3.43 | 3.65 | 3.41 | 3.43 | 3.68
3.84 |
| 3,6,6 | bDGlcp | |
| 3,6 | bDGlcp | 4.44 | 3.23 | 3.39 | 3.30 | 3.55 | 3.77
4.15 |
| 3,3,4,4 | aDGlcp | 4.90 | 3.76 | 3.84 | 3.54 | 3.93 | 3.62
3.78 |
| 3,3 | aDGlcp | |
| 3 | bDGlcp | 4.68 | 3.28 | 3.57 | 3.41 | 3.43 | 3.77
4.15 |
| | bDGlcp | 4.72 | 3.43 | 3.65 | 3.41 | 3.43 | 3.68
3.84 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,4 | aDGlcp | 99.73 | 71.50 | 73.23 | 76.73 | 70.38 | 60.44 |
| 3,6,6,3 | bDGlcp | 102.17 | 72.85 | 84.26 | 69.46 | 75.89 | 60.69 |
| 3,6,6 | bDGlcp | |
| 3,6 | bDGlcp | 102.97 | 73.03 | 75.56 | 69.29 | 74.88 | 68.79 |
| 3,3,4,4 | aDGlcp | 98.12 | 71.14 | 73.44 | 71.71 | 69.57 | 60.98 |
| 3,3 | aDGlcp | |
| 3 | bDGlcp | 102.68 | 74.30 | ? | 69.46 | 75.89 | 68.79 |
| | bDGlcp | 102.17 | 72.85 | 84.26 | 69.46 | 75.89 | 60.69 |
|
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
