Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: cell wall
NCBI PubMed ID: 11711064Publication DOI: 10.1016/s0031-9422(98)00244-1Journal NLM ID: 0151434Publisher: Elsevier
Institutions: Scottish Crop Research Institute, Invergowrie, UK
A scheme is presented for the fractionation of plant cell walls and treated plant cell walls (fibres) after suspending/dissolving in >99% trifluoroacetic acid (TFA), with oat straw and crystalline cellulose being given as examples. While cleavage of covalent bonds occurs, particularly in the non-cellulosic polysaccharides, many bonds are not hydrolysed and fragments have been identified in which covalent bonds remain between carbohydrates and phenolic components. Cellulose chains do not undergo extensive hydrolysis, even after being dissolved in TFA for at least 8 days. However, CP/MAS NMR and DRIFT IR spectroscopy both confirm that the secondary and tertiary structures of regenerated cellulose are severely modified. By analogy with the crystalline cellulose sample, all the cellulose from the oat straw should be present in the material which is soluble in TFA but is precipitated on addition of water (fraction II). The DRIFT spectrum of oat straw fraction II confirms the presence of non-cellulosic components as well. Most of the lignin from the straw is present in the fraction not soluble in TFA.
cellulose, phenolics, Gramineae, Avena sativa, oat straw, non-cellulosic polysaccharides, trifluoroacetic acid, DRIFT spectroscopy, CP/MAS NMR spectroscopy
Structure type: homopolymer
Location inside paper: Abstract
Trivial name: cellulose, β-(1,4)-glucan, cellulose, β-(1,4)-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: centrifugation, TFA hydrolysis, 13C CPMAS NMR, DRIFT IR
Comments, role: essential for cell structure, serves as template for lignin; does not undergo extensive hydrolysis after dissolution in TFA, but secondary and tertiary structures of regenerated cellulose are severely modified
NCBI Taxonomy refs (TaxIDs): 4498Reference(s) to other database(s): GTC:G75830OM, GlycomeDB:
27885, CCSD:
50051, CBank-STR:4229
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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