Aziz AA, Edwards CA, Lean ME, Crozier A Absorption and excretion of conjugated flavonols, including quercetin-4'-O-beta-glucoside and isorhamnetin-4'-O-beta-glucoside by human volunteers after the consumption of onions Free Radical Research29(3) (1998)
257-269
/Variants 0/-Quercetin
/Variants 0/ is:
b-D-Glcp-(1-4')-
OR (inclusively)
b-D-Glcp-(1-3)-
NCBI PubMed ID:9802557 Publication DOI:10.1080/10715769800300291 Journal NLM ID:9423872 Publisher: London: Informa Healthcare Correspondence: Crozier A <a.crozierbio.gla.ac.uk> Institutions: Plant Molecular Science Group, Bower Building, Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK, University of Glasgow Department of Human Nutrition, Yorkhill Hospitals, Glasgow, UK, University of Glasgow Department of Human Nutrition, Queen Elizabeth Building, Royal Infirmary, Glasgow, UK
Flavonols are polyphenols found ubiquitously in plants and plant-products. Flavonols, particularly quercetin, are potent antioxidants in vitro and their intake has been associated inversely with the incidence of coronary heart disease. The aim of this study was to investigate the accumulation in plasma and excretion in urine of flavonol glucosides following ingestion of lightly fried onions. Five healthy volunteers followed a low-flavonoid diet for 3 days. On day 4, after an overnight fast, subjects were given 300 g of lightly fried yellow onions which contain conjugates of quercetin and isorhamnetin, including quercetin-3,4 '-diO-beta-glucoside, isorhamnetin-4'-O-beta-glucoside and quercetin-4'-O-beta-glucoside. Blood collection was carried out at 0 min, 0.5, 1.0, 1.5, 2, 3, 4, 5 and 24h after the supplement. In addition, subjects collected all their urine for 24h following the onion supplement. Isorhamnetin-4'-O-beta-glucoside and quercetin-4 '-O-beta-glucoside accumulated in plasma with maximum levels, defined as proportion of intake, of 10.7+/-2.6% and 0.13+/-0.03% respectively. The time of the quercetin-4'glucoside peak plasma concentration was 1.3+/-0.2 h after the ingestion of onions while a value of 1.8+/-0.7 h was obtained for isorhamnetin-4'-glucoside. Excretion in urine, as a proportion of intake, was 17.4+/-8.3% for isorhamnetin-4'-O-beta-glucoside and 0.2+/-0.1% for quercetin-4'-O-beta-glucoside. Possible reasons for the accumulation and excretion of isorhamnetin-4'-glucoside in proportionally much higher amounts than quercetin-4'-glucoside are discussed. It is concluded that flavonols are absorbed into the bloodstream as glucosides and minor structural differences affect markedly both the level of accumulation and the extent to which the conjugates are excreted.
flavonol conjugates, quercetin-4'-O-β-glucoside, isorhamnetin-4'-O-β-glucoside, accumulation in plasma, excretion in urine
Methods: HPLC, biological assay, acid hydrolisys Biological activity: excretion percentage for quercetin-4'-glucoside is 0.2%; quercetin-4'-glucoside accumulates in the bloodstream and are excreted in urine without seemingly undergoing structural modification
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