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1. (CSDB ID: 64768) | report error |
| a-D-Manp-(1-3)-+ a-L-Fucp-(1-3)-+ | | b-D-Xylp-(1-2)-b-D-Manp-(1-4)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc-(1--/(->3) Asn23-protein/ | a-D-Manp-(1-6)-+ | Show graphically |
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Ulex europaeus
(NCBI TaxID 3902,
species name lookup)
usask.caThe tertiary and quaternary structure of the lectin I from Ulex europaeus (UE-I) has been determined to 2.2 Å resolution. UE-I is a dimeric metalloglycoprotein that binds the H-type 2 human blood group determinant [α-L-Fuc-α(1→2)-β-D-Galβ(1→4)-β-D-GlcNAcα-]. Nine changes from the published amino acid sequence were necessary to account for the electron density. The quaternary structural organization of UE-I is that of the most commonly occurring legume lectin dimer. The tertiary structure of the monomeric subunits is similar to that in the conventional lectin subunit; however, some structural differences are noted. These differences include a four-stranded anti-parallel "S" sheet in UE-I versus the five-stranded S sheet in other lectin monomers. The Ala residue of the Ala-Asp cis-peptide bond present in the carbohydrate-binding site of the conventional lectin monomer is replaced with a Thr in the UE-I structure. Also, a novel disulfide bridge linking Cys115 and Cys150 is present. There are two metallic ions, one calcium and the other manganese, per subunit. N-linked oligosaccharides are at residues 23 and 111 of each subunit. One molecule of R-2-methyl-2, 4-pentanediol (R-MPD) is present in a shallow depression on the surface of each subunit. In order to examine the binding of the H-type 2 blood group determinant by UE-I, its beta-methyl glycoside (H-type 2-OMe) was docked into the binding site of R-MPD. The epitope previously identified for H-type 2-OMe by chemical mapping proved, with only minor adjustment of amino acid residues, to be complementary to the shallow cavity occupied by R-MPD in the structure. Several key interactions have been proposed between the H-type 2-OMe and UE-I.
Structure type: oligomer|
2. (CSDB ID: 64769) | report error |
| a-L-Fucp-(1-3)-b-D-GlcpNAc-(1-4)-b-D-GlcpNAc-(1--/(->3) Asn111-protein/ | Show graphically |
|
Show legend Show as text |
Ulex europaeus
(NCBI TaxID 3902,
species name lookup)
usask.caThe tertiary and quaternary structure of the lectin I from Ulex europaeus (UE-I) has been determined to 2.2 Å resolution. UE-I is a dimeric metalloglycoprotein that binds the H-type 2 human blood group determinant [α-L-Fuc-α(1→2)-β-D-Galβ(1→4)-β-D-GlcNAcα-]. Nine changes from the published amino acid sequence were necessary to account for the electron density. The quaternary structural organization of UE-I is that of the most commonly occurring legume lectin dimer. The tertiary structure of the monomeric subunits is similar to that in the conventional lectin subunit; however, some structural differences are noted. These differences include a four-stranded anti-parallel "S" sheet in UE-I versus the five-stranded S sheet in other lectin monomers. The Ala residue of the Ala-Asp cis-peptide bond present in the carbohydrate-binding site of the conventional lectin monomer is replaced with a Thr in the UE-I structure. Also, a novel disulfide bridge linking Cys115 and Cys150 is present. There are two metallic ions, one calcium and the other manganese, per subunit. N-linked oligosaccharides are at residues 23 and 111 of each subunit. One molecule of R-2-methyl-2, 4-pentanediol (R-MPD) is present in a shallow depression on the surface of each subunit. In order to examine the binding of the H-type 2 blood group determinant by UE-I, its beta-methyl glycoside (H-type 2-OMe) was docked into the binding site of R-MPD. The epitope previously identified for H-type 2-OMe by chemical mapping proved, with only minor adjustment of amino acid residues, to be complementary to the shallow cavity occupied by R-MPD in the structure. Several key interactions have been proposed between the H-type 2-OMe and UE-I.
Structure type: oligomer| New query | Export IDs | Home | Help |
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