Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: bud; leaf; leaf
Publication DOI: 10.1016/S0003-2670(00)01099-0Journal NLM ID: 0370534Correspondence: Ye J <jnye
ecnu.edu.cn>
Institutions: Department of Chemistry, East China Normal University, Shanghai, China, Shanghai SIIC-SMU Bio-Technique Co. Ltd., Shanghai, China
A method based on capillary electrophoresis with electrochemical detection (CE-ED) was developed for the determination of daidzein, rutin and quercetin. The effects of some important factors such as the acidity and concentration of running buffer, separation voltage, injection time, and detection potential were investigated to acquire the optimum conditions. The working electrode was a 300 μm diameter carbon disc electrode positioned opposite the outlet of capillary. The three analytes could be well separated within 10 min in a 40 cm length capillary at a separation voltage of 12 kV in a 100 mmol/l borate buffer (BB, pH 9.0). The response was linear over three orders of magnitude with detection limits (S/N=3) ranged from 0.190 to 0.434 μmol/l for all compounds. This proposed method demonstrated good long-term stability and reproducibility with relative standard deviations (R.S.D.) of less than 5% for both migration time and peak current (n=7). It has been successfully applied for the determination of rutin and quercetin in Chinese traditional drug, Flos Sophorae buds and in the leaves of Ligustrum lucidum Ait. and Cinnamomum camphora (L.) Presl.
capillary electrophoresis, quercetin, rutin, daidzein, electrochemical detection
Structure type: oligomer
C
27H
30O
16Location inside paper: abstract
Trivial name: rutin, rutoside, rutin, quercetin rutinoside, rutoside, quercetin 3-O-rutinose, quercetin-3-O-rutinoside, quercetin 3-O-rutinoside
Compound class: saponin glycoside, glycoside, flavonoid glycoside, flavonol glycoside, flavone glycoside
Contained glycoepitopes: IEDB_136105,IEDB_142488,IEDB_144144,IEDB_146664,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: extraction, capillary electrophoresis (CE), evaporation
NCBI Taxonomy refs (TaxIDs): 3897,
458695,
13429Reference(s) to other database(s): CCSD:
50720, CBank-STR:3399
Show glycosyltransferases
There is only one chemically distinct structure:
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(later renamed to: Styphnolobium japonicum)