Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
NCBI PubMed ID: 10921853Publication DOI: 10.1016/s0141-8130(00)00128-8Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: Courtois J <josiane.courtois
iut.u-picardie.fr>
Institutions: Department de Biologie Appliquee, Université de Picardie Jules Verne, Amiens, France, Centre de Recherche sur les Macromolécules Végétales (CNRS), Grenoble, France
The Rhizobium sp. T1 strain, which induces nodule formation on alfalfa and clover roots, produces, during growth, an extracellular polysaccharide composed of d-glucose and d-glucuronic acid noted glucoglucuronan. During the bacterial growth, the pH of the medium decreases slightly. The control of pH in the growth medium slightly reduces the glucoglucuronan production. Under the conditions tested in the present work, the weight-average molecular weight of the polymers produced with or without pH control are similar: Mw ≈ 2000000; the repeating unit determined by chemical and NMR analyses corresponds to the disaccharide: →3)-β-d-GlcpA-(1→4)-β-d-Glcp-(1→.
polysaccharide, chemical structure, Rhizobium sp.
Structure type: polymer chemical repeating unit ; 2000000
Compound class: polysaccharide, glucoglucuronan
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_149553,IEDB_423153,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, NMR-2D, methylation, GLC-MS, acid hydrolysis, GLC, HPAEC, EI-MS, acetylation, methylation analysis, SEC, reduction, cell growth, dialysis, enzymatic assay, derivatization, Gerwig method, optical density measurement
NCBI Taxonomy refs (TaxIDs): 379
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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