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Kaname N, Zhang J, Meng Z, Xu S, Sugahara K, Doi Y, Kodama H
Effect of timosaponin E1 and E2 on superoxide generation induced by various stimuli in human neutrophils and on platelet aggregation in human blood
Clinica Chimica Acta 295(1-2) (2000)
129-140
|
b-D-Glcp-(1-23)-+
|
b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst
Subst = timosaponin E1 aglycon = SMILES O{3}[C@H]1CC[C@@]2(C)[C@H](CCC3C2CC[C@@]4(C)C3{15}[C@@H](O)C5C4[C@H](C){19}C(O)(CCC({23}CO)C)O5)C1 |
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Anemarrhena asphodeloides
(NCBI TaxID 59045,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: rhizoma
NCBI PubMed ID: 10767399Publication DOI: 10.1016/s0009-8981(00)00196-0Journal NLM ID: 1302422Publisher: Amsterdam: Elsevier
Correspondence: hkodama
kochi-ms.ac.jp
Institutions: Department of Geriatrics, Kochi Medical School, Nankoku-shi, Japan, Department of Chemical & Petroleum Engineering, University of Pittsburgh, Pittsburgh, USA, Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang, China, Department of Chemistry, Kochi Medical School, Nankoku-shi, Japan
We have reported that six steroidal saponins isolated from Anemarrhenae rhizoma had various effects on stimulus-induced superoxide generation in human neutrophils. In this paper, two novel steroidal saponins, timosaponins E1 and E2 were isolated from Anemarrhenae rhizoma, and the effects of these steroidal saponins on superoxide generation in human neutrophils were investigated. Timosaponins E1 and E2 significantly inhibited N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in a concentration-dependent manner, but not that induced by arachidonic acid (AA). On the other hand, both compounds enhanced superoxide generation induced by phorbol 12-myristate 13-acetate (PMA) in a concentration-dependent manner. The superoxide generation induced by PMA with timosaponins E1 and E2 was suppressed by staurosporine, an inhibitor of protein kinase C, but was not suppressed by genistein, an inhibitor of protein tyrosine kinase. Tyrosyl phosphorylation of a 58 kDa protein, which was increased by fMLP, was inhibited by timosaponins E1 and E2. Timosaponins E1 and E2 also inhibited the generation of a 47 kDa protein and platelet aggregation in human blood. The results suggest that protein tyrosine kinase participates in fMLP-mediated superoxide generation by timosaponin E1- and E2-treated human neutrophils.
Neutrophils, Anemarrhenae rhizoma, platelet aggregation, superoxide, timosaponin
Structure type: oligomer
Location inside paper: p. 133, Fig. 1, A
Trivial name: timosaponin E1
Compound class: glycoside
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_153218,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: SDS-PAGE, biological assays, ROS measurement, antibody binding assay
Related record ID(s): 65921
NCBI Taxonomy refs (TaxIDs): 59045
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There is only one chemically distinct structure:
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Kaname N, Zhang J, Meng Z, Xu S, Sugahara K, Doi Y, Kodama H
Effect of timosaponin E1 and E2 on superoxide generation induced by various stimuli in human neutrophils and on platelet aggregation in human blood
Clinica Chimica Acta 295(1-2) (2000)
129-140
|
b-D-Glcp-(1-23)-+
|
b-D-Glcp-(1-2)-b-D-Galp-(1-3)-Subst19Me
Subst = timosaponin E1 aglycon = SMILES O{3}[C@H]1CC[C@@]2(C)[C@H](CCC3C2CC[C@@]4(C)C3{15}[C@@H](O)C5C4[C@H](C){19}C(O)(CCC({23}CO)C)O5)C1 |
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Anemarrhena asphodeloides
(NCBI TaxID 59045,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: rhizoma
NCBI PubMed ID: 10767399Publication DOI: 10.1016/s0009-8981(00)00196-0Journal NLM ID: 1302422Publisher: Amsterdam: Elsevier
Correspondence: hkodama
kochi-ms.ac.jp
Institutions: Department of Geriatrics, Kochi Medical School, Nankoku-shi, Japan, Department of Chemical & Petroleum Engineering, University of Pittsburgh, Pittsburgh, USA, Department of Phytochemistry, Shenyang Pharmaceutical University, Shenyang, China, Department of Chemistry, Kochi Medical School, Nankoku-shi, Japan
We have reported that six steroidal saponins isolated from Anemarrhenae rhizoma had various effects on stimulus-induced superoxide generation in human neutrophils. In this paper, two novel steroidal saponins, timosaponins E1 and E2 were isolated from Anemarrhenae rhizoma, and the effects of these steroidal saponins on superoxide generation in human neutrophils were investigated. Timosaponins E1 and E2 significantly inhibited N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in a concentration-dependent manner, but not that induced by arachidonic acid (AA). On the other hand, both compounds enhanced superoxide generation induced by phorbol 12-myristate 13-acetate (PMA) in a concentration-dependent manner. The superoxide generation induced by PMA with timosaponins E1 and E2 was suppressed by staurosporine, an inhibitor of protein kinase C, but was not suppressed by genistein, an inhibitor of protein tyrosine kinase. Tyrosyl phosphorylation of a 58 kDa protein, which was increased by fMLP, was inhibited by timosaponins E1 and E2. Timosaponins E1 and E2 also inhibited the generation of a 47 kDa protein and platelet aggregation in human blood. The results suggest that protein tyrosine kinase participates in fMLP-mediated superoxide generation by timosaponin E1- and E2-treated human neutrophils.
Neutrophils, Anemarrhenae rhizoma, platelet aggregation, superoxide, timosaponin
Structure type: oligomer
Location inside paper: p. 133, Fig. 1, B
Trivial name: timosaponin E2
Compound class: glycoside
Contained glycoepitopes: IEDB_136044,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_153218,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: SDS-PAGE, biological assays, ROS measurement, antibody binding assay
Related record ID(s): 65920
NCBI Taxonomy refs (TaxIDs): 59045
Show glycosyltransferases
There is only one chemically distinct structure:
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