Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
Journal NLM ID: 7613422WWW link: http://nopr.niscpr.res.in/handle/123456789/22492Publisher: New Delhi, Council of Scientific & Industrial Research
Institutions: Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India, R&D Center, Indian Herbs Ltd., Saharanpur, India
Members of the genus Wrightia, viz. W. tinctoria, W. tomentosa and W. coccinea, have been investigated for their chemical constituents with a view to locating their bioactive principles. Indigotin 3, indirubin 6, tryptanthrin 8, isatin 5, anthranillate 7 and rutin 9 have been isolated and identified as major constituents of W. tinctoria and W. tomentosa. Anthranillate 7 and rutin 9 are the major constituents of W. coccinea. The identities of these compounds have been established by comprehensive chromatographic (HPTLC, HPLC) and spectroscopic (UV-VIS, IR, EI-MS) techniques, using markers and by synthesis, where possible. While indigotin is found to be native in the living plants (in fresh leaves), indirubin was found to be an artifact formed only during drying process after harvesting of the leaves. This transformation is presumably caused by the intact hydrolytic enzyme system(s) and by autoxidation. Seasonal variation studies of the chemical constituents of leaves, using HPTLC and HPLC analyses, revealed similar variation patterns in the three species. The concentration of indigotin-indirubin combination steadily increases from August to November. In contrast, concentration of isatin and anthranillate increases in the months of December and January, at the expense of indigotin-indirubin. Isatin is produced by the autoxidation of indigotin. Tryptanthrin concentration also increases, periodically, in May (at the expense of isatin) and in January. Plausible pathways for the formation of these indole metabolites are appraised on the basis of circumstantial and synthetic evidence.
flavonoids, indican, indole constituents, Wrightia genus
Structure type: monomer
Location inside paper: p. 128, Scheme III, structure 1
Trivial name: indican
Compound class: glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: IR, UV, spectrophotometry, HLC
Related record ID(s): 67015
NCBI Taxonomy refs (TaxIDs): 653463,
144546
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
Journal NLM ID: 7613422WWW link: http://nopr.niscpr.res.in/handle/123456789/22492Publisher: New Delhi, Council of Scientific & Industrial Research
Institutions: Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India, R&D Center, Indian Herbs Ltd., Saharanpur, India
Members of the genus Wrightia, viz. W. tinctoria, W. tomentosa and W. coccinea, have been investigated for their chemical constituents with a view to locating their bioactive principles. Indigotin 3, indirubin 6, tryptanthrin 8, isatin 5, anthranillate 7 and rutin 9 have been isolated and identified as major constituents of W. tinctoria and W. tomentosa. Anthranillate 7 and rutin 9 are the major constituents of W. coccinea. The identities of these compounds have been established by comprehensive chromatographic (HPTLC, HPLC) and spectroscopic (UV-VIS, IR, EI-MS) techniques, using markers and by synthesis, where possible. While indigotin is found to be native in the living plants (in fresh leaves), indirubin was found to be an artifact formed only during drying process after harvesting of the leaves. This transformation is presumably caused by the intact hydrolytic enzyme system(s) and by autoxidation. Seasonal variation studies of the chemical constituents of leaves, using HPTLC and HPLC analyses, revealed similar variation patterns in the three species. The concentration of indigotin-indirubin combination steadily increases from August to November. In contrast, concentration of isatin and anthranillate increases in the months of December and January, at the expense of indigotin-indirubin. Isatin is produced by the autoxidation of indigotin. Tryptanthrin concentration also increases, periodically, in May (at the expense of isatin) and in January. Plausible pathways for the formation of these indole metabolites are appraised on the basis of circumstantial and synthetic evidence.
flavonoids, indican, indole constituents, Wrightia genus
Structure type: oligomer
C
27H
30O
16Location inside paper: p. 127, structure 9
Trivial name: rutin, rutoside, rutin, quercetin rutinoside, rutoside, quercetin 3-O-rutinose, quercetin-3-O-rutinoside, quercetin 3-O-rutinoside
Compound class: saponin glycoside, glycoside, flavonoid glycoside, flavonol glycoside, flavone glycoside
Contained glycoepitopes: IEDB_136105,IEDB_142488,IEDB_144144,IEDB_146664,IEDB_225177,IEDB_885823,IEDB_983931,SB_192
Methods: IR, UV, spectrophotometry, HLC
Related record ID(s): 67014
NCBI Taxonomy refs (TaxIDs): 653463,
144546,
429302Reference(s) to other database(s): CCSD:
50720, CBank-STR:3399
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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