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Structure type: homopolymer ; n=1-3
Aglycon: Ser66
Compound class: O-glycoprotein, recombinant glycoprotein
Contained glycoepitopes: IEDB_144983,IEDB_983930

• Article ID: 6633
  Olstad OK, Reppe S, Gabrielsen OS, Hartmanis M, Blingsmo OR, Gautvik VT, Haflan AK, Christensen TB, Oyen TB, Gautvik KM "Isolation and characterization of two biologically active O- glycosylated forms of human parathyroid hormone produced in Saccharomyces cerevisiae. Identification of a new motif for O-glycosylation" - European Journal of Biochemistry 205 (1992) 311-319
  

  Expression and secretion of human parathyroid hormone in Saccharomyces cerevisiae were achieved by fusing a cDNA encoding the mature human parathyroid hormone (hPTH) to the preproregion of the yeast mating factor α. Purified hPTH from yeast-culture medium was found to contain, in addition to the native unglycosylated form, two mannosylated variants with different molecular masses. The three hPTH forms were processed identically, resulting in the same 84 amino acid polypeptides with amino acid sequences identical to the native hormone. In both the O-glycosylated forms that were separated by isocratic reverse-phase HPLC, two mannose-linked residues were localized to Thr79. In addition, the most glycosylated form showed a heterogeneous modification of three, four or five mannosyl residues linked at Ser66. Lysine is N-terminally located to Ser66 and probably stimulates this glycosylation, which introduces a possible new motif for O-glycosylation in yeast. The two glycosylated forms of hPTH had similar biological activity which was identical to the native form of hPTH in a hormone-sensitive adenylate cyclase assay in bone sarcoma cells. Thus, a C-terminal O-glycosylation of hPTH with up to seven mannosyl residues/molecule did not affect the biological activity of the hormone, making possible production of hPTH with potential different pharmacokinetic properties.

  NCBI PubMed ID: 1555591
  Journal NLM ID: 0107600
  Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
  Institutions: Institute of Medical Biochemistry, University of Oslo, Norway
  Methods: SDS-PAGE, MS, reverse-phase HPLC, cation-exchange chromatography, proteolytic digestion
  
   
  
  Saccharomyces cerevisiae BJ1991
  CSDB ID 123785 (all data & tools)

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Structure type: structural motif or average structure ; 28000
Compound class: polysaccharide, galactoglucomannan
Contained glycoepitopes: IEDB_130701,IEDB_134623,IEDB_134624,IEDB_136044,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_1394181,IEDB_1394182,IEDB_140116,IEDB_140628,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144144,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153201,IEDB_153543,IEDB_156493,IEDB_190606,IEDB_225177,IEDB_241101,IEDB_885823,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88

• Article ID: 8188
  He Y, Ye M, Du Z, Wang H, Wu Y, Yang L "Purification, characterization and promoting effect on wound healing of an exopolysaccharide from Lachnum YM405" - Carbohydrate Polymers 105 (2014) 169-176
  

  An exopolysaccharide (LEP-2b) with molecular weight of 2.8×10(4)Da was isolated from Lachnum YM405 and purified by DEAE-cellulose 52, Sepharose CL-6B chromatographic column. It consisted of rhamnose (Rha), mannose (Man), glucose (Glc) and galactose (Gal) in a molar ratio of 1.0:5.0:11.5:12.5. Its backbone consisted of →4)-β-D-Manp-(1→, →2)-α-d-Rhap-(1→, →6)-β-D-Glcp-(1→, and →6)-α-d-Galp-(1→, and three types of branches were composed of →6)-α-d-1-OMe-Manf-(2→, →6)-β-D-1-OMe-Manf-(2→, →1)-α-d-Galp-(6→, →1)-β-D-Galp-(6→, and →1)-β-D-Glcp, which were at O-3 of 1,3,6-linked α-d-Manp and O-2, O-3 of the same 1,2,3,6-linked β-D-Glcp in the backbone respectively. LEP-2b ointment significantly accelerated the decrustation of the wounded skin, shortened the healing time and increased the water and hydroxyproline contents of the healed skin. Combined with the results of macroscopic and histological observations, we deemed that LEP-2b could inhibit inflammatory reaction of scalded skin, accelerate tissue repair and re-epithelialization, thereby playing a positive role in promoting wound healing.

  heteropolysaccharide, purification, Structural characterization, wound healing, Lachnum, scald

  NCBI PubMed ID: 24708966
  Publication DOI: 10.1016/j.carbpol.2014.01.080
  Journal NLM ID: 8307156
  Publisher: Elsevier
  Correspondence: He Y <845212742@qq.com>; Ye M ; Du Z <173586166@qq.com>; Wang H ; Wu Y <912446078@qq.com>; Yang L
  Institutions: School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China
  Methods: 13C NMR, 1H NMR, periodate oxidation, GC-MS, acid hydrolysis, Smith degradation, GPC, UV, statistical analysis, methylation analysis, reduction with NaBH4, HPGPC, FT-IR, TFA hydrolysis
  
   
  
  Lachnum YM405
  CSDB ID 47463 (all data & tools)

Show legend Show as text

Structure type: structural motif or average structure
Compound class: polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_134623,IEDB_134624,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_1394181,IEDB_140116,IEDB_140628,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153201,IEDB_153543,IEDB_156489,IEDB_156493,IEDB_156557,IEDB_190606,IEDB_241101,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88

• Article ID: 8517
  He Y, Ye M, Jing L, Du Z, Surahio M, Xu H, Li J "Preparation, characterization and bioactivities of derivatives of an exopolysaccharide from Lachnum" - Carbohydrate Polymers 117 (2015) 788-796
  

  An exopolysaccharide, obtained previously LEP-2b from Lachnum YM405, was phosphated and sulfated successfully. The derivatives named PLEP-2b and SLEP-2b, respectively, and their respective degree of substitution were 0.174 and 0.431. Phosphate groups -PO3H2 substituted at C-6 of 1,4-β-D-mannopyranose, C-5 of 2,6-β-D-1-OMe-mannofuranoside, C-3 of 1,6-β-D-galactopyranose, C-2 of 1-β-D-glucopyranose, and C-6 of 1,2-α-D-rhampyranose, while sulfate groups SO3H were mainly at C-6 of 1,4-β-D-Manp, C-6 of 1-β-D-Glcp and C-6 of 1,2-α-D-Rhap. Compared with LEP-2b, the scavenging effects of the derivatives, on hydroxyl radical and superoxide anion were significantly increased after the modifications, except for reducing power. Meanwhile, phosphorylated and sulfated modifications remarkably strengthened the inhibiting effect of LEP-2b on the proliferation of CT-26 murine colon carcinoma, Lewis lung carcinoma and human hepatocellular carcinoma HepG2 cells. The derivatives significantly enhanced the antioxidant and antitumor activities in vitro. Compared with sulfation, phosphorylation improved the inhibitory effect more contraposingly on some specific tumor cells

  phosphorylation, sulfation, Antioxidant activity, antitumor activity, Lachnum polysaccharide

  NCBI PubMed ID: 25498701
  Publication DOI: 10.1016/j.carbpol.2014.10.046
  Journal NLM ID: 8307156
  Publisher: Elsevier
  Correspondence: Ye M
  Institutions: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China, College of Medical Engineering, Hefei University of Technology, Hefei, Anhui, China
  Methods: 13C NMR, IR, 31P NMR, UV, colorimetry, dialysis, reducing sugar assays, precipitation, radical scavenging assay, derivatization, antitumor activity assay, centrifugation
  
   
  
  Lachnum sp. YM405
  CSDB ID 48356 (all data & tools)

Show legend Show as text

Structure type: structural motif or average structure
Compound class: polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_134623,IEDB_134624,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_1394181,IEDB_140116,IEDB_140628,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153201,IEDB_153543,IEDB_156489,IEDB_156493,IEDB_156557,IEDB_190606,IEDB_241101,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88

• Article ID: 8517
  He Y, Ye M, Jing L, Du Z, Surahio M, Xu H, Li J "Preparation, characterization and bioactivities of derivatives of an exopolysaccharide from Lachnum" - Carbohydrate Polymers 117 (2015) 788-796
  

  An exopolysaccharide, obtained previously LEP-2b from Lachnum YM405, was phosphated and sulfated successfully. The derivatives named PLEP-2b and SLEP-2b, respectively, and their respective degree of substitution were 0.174 and 0.431. Phosphate groups -PO3H2 substituted at C-6 of 1,4-β-D-mannopyranose, C-5 of 2,6-β-D-1-OMe-mannofuranoside, C-3 of 1,6-β-D-galactopyranose, C-2 of 1-β-D-glucopyranose, and C-6 of 1,2-α-D-rhampyranose, while sulfate groups SO3H were mainly at C-6 of 1,4-β-D-Manp, C-6 of 1-β-D-Glcp and C-6 of 1,2-α-D-Rhap. Compared with LEP-2b, the scavenging effects of the derivatives, on hydroxyl radical and superoxide anion were significantly increased after the modifications, except for reducing power. Meanwhile, phosphorylated and sulfated modifications remarkably strengthened the inhibiting effect of LEP-2b on the proliferation of CT-26 murine colon carcinoma, Lewis lung carcinoma and human hepatocellular carcinoma HepG2 cells. The derivatives significantly enhanced the antioxidant and antitumor activities in vitro. Compared with sulfation, phosphorylation improved the inhibitory effect more contraposingly on some specific tumor cells

  phosphorylation, sulfation, Antioxidant activity, antitumor activity, Lachnum polysaccharide

  NCBI PubMed ID: 25498701
  Publication DOI: 10.1016/j.carbpol.2014.10.046
  Journal NLM ID: 8307156
  Publisher: Elsevier
  Correspondence: Ye M
  Institutions: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China, College of Medical Engineering, Hefei University of Technology, Hefei, Anhui, China
  Methods: 13C NMR, IR, 31P NMR, UV, colorimetry, dialysis, reducing sugar assays, precipitation, radical scavenging assay, derivatization, antitumor activity assay, centrifugation
  
   
  
  Lachnum sp. YM405
  CSDB ID 48355 (all data & tools)

Show legend Show as text

Structure type: structural motif or average structure ; 28000
Compound class: polysaccharide
Contained glycoepitopes: IEDB_130701,IEDB_134623,IEDB_134624,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_1394181,IEDB_140116,IEDB_140628,IEDB_141794,IEDB_141806,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153201,IEDB_153543,IEDB_156489,IEDB_156493,IEDB_156557,IEDB_190606,IEDB_241101,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_44,SB_67,SB_7,SB_72,SB_88

• Article ID: 8517
  He Y, Ye M, Jing L, Du Z, Surahio M, Xu H, Li J "Preparation, characterization and bioactivities of derivatives of an exopolysaccharide from Lachnum" - Carbohydrate Polymers 117 (2015) 788-796
  

  An exopolysaccharide, obtained previously LEP-2b from Lachnum YM405, was phosphated and sulfated successfully. The derivatives named PLEP-2b and SLEP-2b, respectively, and their respective degree of substitution were 0.174 and 0.431. Phosphate groups -PO3H2 substituted at C-6 of 1,4-β-D-mannopyranose, C-5 of 2,6-β-D-1-OMe-mannofuranoside, C-3 of 1,6-β-D-galactopyranose, C-2 of 1-β-D-glucopyranose, and C-6 of 1,2-α-D-rhampyranose, while sulfate groups SO3H were mainly at C-6 of 1,4-β-D-Manp, C-6 of 1-β-D-Glcp and C-6 of 1,2-α-D-Rhap. Compared with LEP-2b, the scavenging effects of the derivatives, on hydroxyl radical and superoxide anion were significantly increased after the modifications, except for reducing power. Meanwhile, phosphorylated and sulfated modifications remarkably strengthened the inhibiting effect of LEP-2b on the proliferation of CT-26 murine colon carcinoma, Lewis lung carcinoma and human hepatocellular carcinoma HepG2 cells. The derivatives significantly enhanced the antioxidant and antitumor activities in vitro. Compared with sulfation, phosphorylation improved the inhibitory effect more contraposingly on some specific tumor cells

  phosphorylation, sulfation, Antioxidant activity, antitumor activity, Lachnum polysaccharide

  NCBI PubMed ID: 25498701
  Publication DOI: 10.1016/j.carbpol.2014.10.046
  Journal NLM ID: 8307156
  Publisher: Elsevier
  Correspondence: Ye M
  Institutions: Microbial Resources and Application Laboratory, College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China, College of Medical Engineering, Hefei University of Technology, Hefei, Anhui, China
  Methods: 13C NMR, IR, 31P NMR, UV, colorimetry, dialysis, reducing sugar assays, precipitation, radical scavenging assay, derivatization, antitumor activity assay, centrifugation
  
   
  
  Lachnum sp. YM405
  CSDB ID 48354 (all data & tools)