Found 33 structures.
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1. Compound ID: 1922
Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_225177,IEDB_423153,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 599
An J, Carlson RW, Glushka J, Streeter JG "The structure of a novel polysaccharide produced by Bradyrhizobium species within soybean nodules" -
Carbohydrate Research 269(2) (1995) 303-317
Certain strains of Bradyrhizobium japonicum and B. elkanii produce a polysaccharide within the root nodules of their legume host, soybean. These nodule polysaccharides (NPSs) were isolated and characterized. The NPS produced by B. elkanii strains proved to be identical in glycosyl composition and linkages to the extracellular polysaccharide (EPS) of this species indicating that the NPS and EPS for B. elkanii have identical structures (W.F. Dudman, Carbohydr. Res., 66 (1978) 9-23), →4)[O-Me-b-D-GlcAp-(1→3)]-a-L-Rhap-(1→3)-b-L-Rhap-(1→4)-b-L-Rhap-(1→. However, the structure of the NPS from B. japonicum proved to be quite different from that of its EPS. Methylation analysis of this NPS showed that it consists of 3-linked Gal, 3-linked Rha, 2,4-linked Rha, 4-linked Rha, and terminal 2-O-methyl GIcA in a 1:1:1:1:1 ratio. Stereochemical configurations of the glycosyl residues were determined by the preparation and analysis of trimethylsilyl (Me3Si) (-)-2-butyl glycosides. NMR spectroscopy (both 1H and 13C) showed that the Gal residue is a-linked, while all the other glycosyl residues are b-linked. Oligosaccharides produced by periodate oxidation-Smith degradation were purified, as were oligosaccharides produced by partial acid hydrolysis. Characterization of the Smith degradation products by methylation analysis, NMR spectroscopy, electrospray-mass spectrometry, and characterization of the partial acid hydrolysate oligosaccharides showed that the repeating oligosaccharide unit of the NFS has the structure, →3)-a-D-Galp-(1→3)-b-L-Rhap-(1→4)-[2-O-Me-b-GlcAp-(1→2)]b-L-Rhap-(1→4)-b-L-Rhap-(1→.
polysaccharide, Bradyrhizobium japonicum, Bradyrhizobium elkanii, Nodules, soybean
NCBI PubMed ID: 7780995Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Complex Carbohydrate Center, University of Georgia, Athens, GA 30602-4712, USA, Department of Agronomy, The Ohio State University, Wooster, OH, USA
Methods: NMR-2D, ESI-MS, acid hydrolysis, HPAEC, Smith degradation
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2. Compound ID: 3142
Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_141806,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 1146
Robijn GW, van den Berg DJC, Haas H, Kamerling JP, Vliegenthart JFG "Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0-1" -
Carbohydrate Research 276(1) (1995) 117-136
The exopolysaccharide produced by Lactobacillus sake 0-1 in a semi-defined medium was found to have an average molecular mass of 6 x 10(6) Da and a composition of D-glucose, L-rhamnose, and sn-glycerol 3-phosphate (3:2:1). The polysaccharide is partially O-acetylated. By means of partial acid hydrolysis, O-deacetylation, deglycerophosphorylation, methylation analysis, and 1D/2D NMR (1H, 13C, and 31P) studies the polysaccharide was shown to be composed of repeating units with the following structure: [structure]
NMR, structure, polysaccharide, determination, Lactic acid bacteria, exopolysaccharide, Lactobacillus, L-rhamnose, O-acetyl, D-glucose, milk, Lactobacillus sakei, hosphoglycerol
NCBI PubMed ID: 8536250Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands, Unilever Research Laboratory Vlaardingen, P.O. Box 114, NL-3130 AC, Vlaardingen, The Netherlands
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, FAB-MS, partial acid hydrolysis, sugar analysis, dephosphorylation, GLC, anion-exchange chromatography, de-O-acetylation
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3. Compound ID: 3248
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b-D-GlcpA-(1-2)-+
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b-D-Glcp-(1-4)-b-D-Manp-(1-4)-b-D-Glcp-(1-4)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_137485,IEDB_140630,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
The structure is contained in the following publication(s):
- Article ID: 1187
Senchenkova SN, Knirel YA, Likhosherstov LM, Shashkov AS, Shibaev VN, Starukhina LA, Deryabin VV "Structure of simusan, a new acidic exopolysaccharide from Arthrobacter sp" -
Carbohydrate Research 266 (1995) 103-113
Simusan, a major exopolysaccharide produced by an ethanol-utilizing Arthrobacter sp. strain CE-17, contains D-glucose, D-mannose, D-galactose, L-rhamnose, D-glucuronic acid, and pyruvic acid in the ratios approximately 3:2:1:1:1:1 as well as O-acyl groups (presumably (presumably residues of acetic and palmitic acid). On the basis of chemical modifications of the polysaccharide, solvolysis with anhydrous hydrogen fluoride resulting in a penta- and an octa-saccharide fragment, Smith degradation, and 1H and 13C NMR analysis, the following structure of the repeating unit was established: [formula: see text] It is suggested that at least one of the glucose residues and the galactose residue are O-acetylated.
structure, acidic, exopolysaccharide, extracellular, 2D NMR, 1H NMR, acidic exopolysaccharide, solvolysis, Arthrobacter, HF
NCBI PubMed ID: 7697646Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: sen@ioc.ac.ru
Institutions: N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow,Russia
Methods: NMR
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4. Compound ID: 3249
|
b-D-GlcpA-(1-2)-+
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b-D-Manp-(1-6)-b-D-Glcp-(1-4)-a-D-Galp-(1-4)-b-D-Glcp-(1-4)-b-D-Manp-(1-4)-b-D-Glcp-(1-4)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 1187
Senchenkova SN, Knirel YA, Likhosherstov LM, Shashkov AS, Shibaev VN, Starukhina LA, Deryabin VV "Structure of simusan, a new acidic exopolysaccharide from Arthrobacter sp" -
Carbohydrate Research 266 (1995) 103-113
Simusan, a major exopolysaccharide produced by an ethanol-utilizing Arthrobacter sp. strain CE-17, contains D-glucose, D-mannose, D-galactose, L-rhamnose, D-glucuronic acid, and pyruvic acid in the ratios approximately 3:2:1:1:1:1 as well as O-acyl groups (presumably (presumably residues of acetic and palmitic acid). On the basis of chemical modifications of the polysaccharide, solvolysis with anhydrous hydrogen fluoride resulting in a penta- and an octa-saccharide fragment, Smith degradation, and 1H and 13C NMR analysis, the following structure of the repeating unit was established: [formula: see text] It is suggested that at least one of the glucose residues and the galactose residue are O-acetylated.
structure, acidic, exopolysaccharide, extracellular, 2D NMR, 1H NMR, acidic exopolysaccharide, solvolysis, Arthrobacter, HF
NCBI PubMed ID: 7697646Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: sen@ioc.ac.ru
Institutions: N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow,Russia
Methods: NMR
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5. Compound ID: 3530
Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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6. Compound ID: 3531
Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_133754,IEDB_136105,IEDB_140630,IEDB_225177,IEDB_423153,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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7. Compound ID: 3535
|
a-L-Rhap-(1-2)-a-L-Rhap-(1-3)-b-D-Galp-(1-2)-a-L-Rhap-(1-4)-b-D-GlcpA-(1-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-2)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_133754,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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8. Compound ID: 3536
|
b-D-Galp-(1-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-4)-b-D-GlcpA-(1-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-3)-b-D-Galp-(1-2)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_133754,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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9. Compound ID: 5012
Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,SB_44,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 1961
Erbing C, Kenne L, Lindberg B, Hammarstrom S "Structure of the O-specific side-chains of the Escherichia coli O75 lipopolysaccharide: a revision" -
Carbohydrate Research 60(2) (1978) 400-403
no abstract available
Publication DOI: 10.1016/S0008-6215(78)80049-4Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Stockholm, Sweden, Department of Immunology, University of Stockholm, Stockholm, Sweden
Methods: 1H NMR, methylation, gel filtration, sugar analysis, acid hydrolysis, GLC, de-N-acetylation/deamination, ion-exchange chromatography, optical rotation measurement
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10. Compound ID: 5413
Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 2261
Gray JSS, Brand JM, Koerner TAW, Montgomery R "Structure of an extracellular polysaccharide produced by Erwinia chrysanthemi" -
Carbohydrate Research 245 (1993) 271-287
Erwinia chrysanthemi pv zeae strain SR260, a phytopathogen of corn, produced from lactose an acidic extracellular polysaccharide which was purified and found to consist of L-rhamnose, D-mannose, D-glucose, and D-glucuronic acid in the ratio of 3:1:1:1. A combination of chemical (carboxyl-group reduction, methylation analysis, periodate oxidation, Smith degradation, and lithium-ethylenediamine degradation) and physical (1 and 2D NMR spectroscopy) methods revealed that the polysaccharide is composed of a hexasaccharide repeating unit 1: [formula: see text]
NCBI PubMed ID: 8370026Publication DOI: 10.1016/0008-6215(93)80077-RJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Biochemistry, University of Fort Hare, Alice, South Africa
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, NMR-2D, carboxyl reduction, Smith degradation, Li/ethylenediamine degradation
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11. Compound ID: 5935
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b-D-Glcp-(1-3)-+
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a-D-Manp-(1-2)-b-D-Manp-(1-3)-b-D-Manp-(1-3)-L-Rha-ol |
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Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 2641
Kocharova NA, Knirel YA, Shashkov AS, Kochetkov NK, Pier GB "Structure of an extracellular cross-reactive polysaccharide from Pseudomonas aeruginosa immunotype 4" -
Journal of Biological Chemistry 263 (1988) 11291-11295
A neutral small molecular mass (approximately 6.5 kDa) polysaccharide comprising a pentasaccharide repeat unit was isolated from culture supernatants of Pseudomonas aeruginosa immunotype 4. The polysaccharide had a pentasaccharide repeating unit as follows (formula; see text) where Rha is rhamnose. The structure was determined using acid hydrolysis, solvolysis with anhydrous hydrogen fluoride, methylation analysis, and 1H and 13C nuclear magnetic resonance spectroscopy including nuclear Overhauser enhancement experiments. The polysaccharide bound antibody raised to the lipopolysaccharide of the seven P. aeruginosa Fisher-Devlin immunotype strains. Inhibition assays demonstrated the presence of a serologically similar polysaccharide in supernatants of these strains. Affinity-purified antibody to the polysaccharide bound to lipopolysaccharide and whole cells of the immunotype strains of P. aeruginosa in a Western immunoblot and colony blot assay, respectively. This polysaccharide seems to contain an antigenic determinant present in the core of the P. aeruginosa lipopolysaccharide or may represent another minor polysaccharide substituent on the lipopolysaccharide in addition to the O side chain.
NCBI PubMed ID: 3136157Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the Union of Soviet Socialist Republics, Moscow, Chunning Laboratory, Department of Medicine, Brighum and Women’s Hospital, Harvard Medical School, Boston. Massachusetts 02115
Methods: 13C NMR, 1H NMR
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12. Compound ID: 6546
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b-D-Galp-(1-3)-+
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b-D-Galp-(1-3)-b-D-Glcp-(1-3)-a-L-Rhap-(1-4)-b-D-Glcp-(1-3)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_136044,IEDB_136105,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 2960
Grimmecke HD, Knirel YA, Kiesel B, Voges M, Rietschel ET "Structure of the Acetobacter methanolicus MB 129 capsular polysaccharide, and of oligosaccharides resulting from degradation by bacteriophage Acm7" -
Carbohydrate Research 259 (1994) 45-58
The capsular polysaccharide of Acetobacter methanolicus MB 129 consists of D-Glc, D-Gal, L-Rha, and L-glyceric acid in the molar ratios 1:1:1:0.3. Periodate oxidation, methylation analysis, solvolysis with HF, and detailed 1H and 13C NMR analysis resulted in the structure of the repeating unit shown below. [formula: see text] Bacteriophage Acm7-associated end-α-L-rhamnopyranoside hydrolase depolymerizes the CPS even in the presence of the O-acyl group, to give the respective hexa-, nona-, and dodeca-saccharides.
NCBI PubMed ID: 8039190Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Forschungsinstitut Borstel, Institut fur Experimentelle Biologie und Medizin, Borstel, Germany
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13. Compound ID: 6547
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b-D-Galp-(1-3)-+ b-D-Galp-(1-3)-+
| |
b-D-Galp-(1-3)-b-D-Glcp-(1-3)-a-L-Rhap-(1-4)-b-D-Glcp-(1-3)-a-L-Rhap-(1-4)-b-D-Glcp-(1-3)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_136044,IEDB_136105,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 2960
Grimmecke HD, Knirel YA, Kiesel B, Voges M, Rietschel ET "Structure of the Acetobacter methanolicus MB 129 capsular polysaccharide, and of oligosaccharides resulting from degradation by bacteriophage Acm7" -
Carbohydrate Research 259 (1994) 45-58
The capsular polysaccharide of Acetobacter methanolicus MB 129 consists of D-Glc, D-Gal, L-Rha, and L-glyceric acid in the molar ratios 1:1:1:0.3. Periodate oxidation, methylation analysis, solvolysis with HF, and detailed 1H and 13C NMR analysis resulted in the structure of the repeating unit shown below. [formula: see text] Bacteriophage Acm7-associated end-α-L-rhamnopyranoside hydrolase depolymerizes the CPS even in the presence of the O-acyl group, to give the respective hexa-, nona-, and dodeca-saccharides.
NCBI PubMed ID: 8039190Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Forschungsinstitut Borstel, Institut fur Experimentelle Biologie und Medizin, Borstel, Germany
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14. Compound ID: 6548
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b-D-Galp-(1-3)-+ b-D-Galp-(1-3)-+ b-D-Galp-(1-3)-+
| | |
b-D-Galp-(1-3)-b-D-Glcp-(1-3)-a-L-Rhap-(1-4)-b-D-Glcp-(1-3)-a-L-Rhap-(1-4)-b-D-Glcp-(1-3)-a-L-Rhap-(1-4)-b-D-Glcp-(1-3)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_136044,IEDB_136105,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 2960
Grimmecke HD, Knirel YA, Kiesel B, Voges M, Rietschel ET "Structure of the Acetobacter methanolicus MB 129 capsular polysaccharide, and of oligosaccharides resulting from degradation by bacteriophage Acm7" -
Carbohydrate Research 259 (1994) 45-58
The capsular polysaccharide of Acetobacter methanolicus MB 129 consists of D-Glc, D-Gal, L-Rha, and L-glyceric acid in the molar ratios 1:1:1:0.3. Periodate oxidation, methylation analysis, solvolysis with HF, and detailed 1H and 13C NMR analysis resulted in the structure of the repeating unit shown below. [formula: see text] Bacteriophage Acm7-associated end-α-L-rhamnopyranoside hydrolase depolymerizes the CPS even in the presence of the O-acyl group, to give the respective hexa-, nona-, and dodeca-saccharides.
NCBI PubMed ID: 8039190Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Forschungsinstitut Borstel, Institut fur Experimentelle Biologie und Medizin, Borstel, Germany
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15. Compound ID: 12364
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a-D-Glcp-(1-3)-+
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a-D-Galp-(1-3)-a-D-FucpNAc-(1-3)-b-L-Rhap-(1-4)-L-Rha-ol |
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Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 4911
Perepelov AV, Filatov AV, Wang M, Shashkov AS, Wang L, Knirel YA "Structure and gene cluster of the O-antigen of Enterobacter cloacae G3421" -
Carbohydrate Research 427 (2016) 55-59
The O-polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3421 and studied by sugar analysis along with 1D and 2D 1H and 13C NMR spectroscopy. In addition, partial solvolysis with anhydrous trifluoroacetic acid was applied, which cleaved selectively the α-l-rhamnopyranosidic linkages. The following structure of the branched hexasaccharide repeating unit was established. [Formula: see text]. The O-polysaccharide studied shares the β-l-Rhap-(1→4)-α-l-Rhap-(1→2)-α-l-Rhap trisaccharide fragment with the O-polysaccharide of Shigella boydii type 18. The O-antigen gene cluster of E. cloacae G3421 was sequenced. Functions of genes in the cluster, including those for glycosyltransferases, were tentatively assigned by a comparison with sequences in the available databases and found to be consistent with the O-polysaccharide structure.
Lipopolysaccharide, bacterial polysaccharide structure, O-antigen gene cluster, Enterobacter cloacae, Solvolytic cleavage
Publication DOI: 10.1016/j.carres.2016.03.008Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: perepel@ioc.ac.ru
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin, China
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, DNA sequencing, sugar analysis, ESI-MS, acid hydrolysis, GLC, NMR-1D, GPC, function analysis of gene clusters, solvolysis with trifluoroacetic acid
- Article ID: 5461
Knirel YA, Naumenko OI, Senchenkova SN, Perepelov AV "Chemical methods for selective cleavage of glycosidic linkages in structural analysis of bacterial polysaccharides" -
Russian Chemical Reviews = Uspekhi Khimii 88(4) (2019) 406-424
This review is devoted to methods for the selective cleavage of glycosidic bonds. The mechanisms of reactions underlying these methods are considered and examples of their practical application in the structural analysis of bacterial polysaccharides are given. Specific methods for the selective cleavage of polysaccharides, remaining relevant for researchers, include the Smith degradation based on destruction of monosaccharides containing vicinal diol groups, dephosphorylation of phosphate-containing polysaccharides with hydrofluoric acid and the hydrolytic cleavage of glycosyl phosphate bonds in the latter compounds. Non-specific methods, including partial acid hydrolysis, acetolysis and solvolysis with anhydrous organic (CF3SO3H, MeSO3H, CF3CO2H) and inorganic (HF) acids do not make any specific demands on the composition and structure of the polysaccharide and are sensitive to its fine structural features. The review addesses the issue of stability of glycosidic bonds in various monosaccharides to reagents used for non-specific selective cleavage.
structural analysis, Bacterial polysaccharide, selective cleavage, glycosidic bond
Publication DOI: 10.1070/RCR4856Journal NLM ID: 0404506Publisher: London: Chemical Society
Correspondence: Yu.A. Knirel
Institutions: N.D. Zelinskii Institute of Organic Chemistry, Russian Academy of Sciences
Methods: partial acid hydrolysis, HF solvolysis, acid hydrolysis, mild acid hydrolysis, alkaline degradation, b-elimination, Smith degradation, deamination, de-O-acetylation, HF treatment, reduction with NaBD4, triflic acid solvolysis, acetolysis, Li/ethylenediamine degradation, hydrazinolysis, reduction with NaBH4, mild acid degradation, trifluoroacetic acid solvolysis, partial solvolysis with trifluoroacetic acid, de-N-acetylation with hydrazine, part acid hydrolysis, HF solvolysis; published polymerization frame was shifted for conformity with other records.
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