Found 44 structures.
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1. Compound ID: 1922
Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_225177,IEDB_423153,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 599
An J, Carlson RW, Glushka J, Streeter JG "The structure of a novel polysaccharide produced by Bradyrhizobium species within soybean nodules" -
Carbohydrate Research 269(2) (1995) 303-317
Certain strains of Bradyrhizobium japonicum and B. elkanii produce a polysaccharide within the root nodules of their legume host, soybean. These nodule polysaccharides (NPSs) were isolated and characterized. The NPS produced by B. elkanii strains proved to be identical in glycosyl composition and linkages to the extracellular polysaccharide (EPS) of this species indicating that the NPS and EPS for B. elkanii have identical structures (W.F. Dudman, Carbohydr. Res., 66 (1978) 9-23), →4)[O-Me-b-D-GlcAp-(1→3)]-a-L-Rhap-(1→3)-b-L-Rhap-(1→4)-b-L-Rhap-(1→. However, the structure of the NPS from B. japonicum proved to be quite different from that of its EPS. Methylation analysis of this NPS showed that it consists of 3-linked Gal, 3-linked Rha, 2,4-linked Rha, 4-linked Rha, and terminal 2-O-methyl GIcA in a 1:1:1:1:1 ratio. Stereochemical configurations of the glycosyl residues were determined by the preparation and analysis of trimethylsilyl (Me3Si) (-)-2-butyl glycosides. NMR spectroscopy (both 1H and 13C) showed that the Gal residue is a-linked, while all the other glycosyl residues are b-linked. Oligosaccharides produced by periodate oxidation-Smith degradation were purified, as were oligosaccharides produced by partial acid hydrolysis. Characterization of the Smith degradation products by methylation analysis, NMR spectroscopy, electrospray-mass spectrometry, and characterization of the partial acid hydrolysate oligosaccharides showed that the repeating oligosaccharide unit of the NFS has the structure, →3)-a-D-Galp-(1→3)-b-L-Rhap-(1→4)-[2-O-Me-b-GlcAp-(1→2)]b-L-Rhap-(1→4)-b-L-Rhap-(1→.
polysaccharide, Bradyrhizobium japonicum, Bradyrhizobium elkanii, Nodules, soybean
NCBI PubMed ID: 7780995Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Complex Carbohydrate Center, University of Georgia, Athens, GA 30602-4712, USA, Department of Agronomy, The Ohio State University, Wooster, OH, USA
Methods: NMR-2D, ESI-MS, acid hydrolysis, HPAEC, Smith degradation
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2. Compound ID: 2282
Structure type: oligomer
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
The structure is contained in the following publication(s):
- Article ID: 765
Cérantola S, Lemassu-Jacquier A, Montrozier H "Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of Burkholderia cepacia - Characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit" -
European Journal of Biochemistry 260(2) (1999) 373-383
The structure of the exopolysaccharide (EPS) produced by a clinical isolate of Burkholderia cepacia isolated from a patient with fibrocystic lung disease has been investigated. By means of methylation analyses, carboxyl reduction, partial depolymerization by fuming HCl and chemical degradations such as Smith degradation, lithiumethylenediamine degradation and beta-elimination, supported by GC/MS and NMR spectroscopic analyses, the repeat unit of the EPS has been identified and was shown to correspond to the acidic branched heptasaccharide with the following structure: [formula: see text]. This partially acetylated acidic polymer, distinguished by the presence of the less usual D-isomer of rhamnose and of a trisubstituted glucuronic acid residue, could represent the main EPS produced by this bacterial species.
clinical, isolate, structural, characterization, Burkholderia, Burkholderia cepacia, repeating unit, acid, NMR spectroscopy, exopolysaccharide, elucidation, heptasaccharide repeating unit, glucuronic acid, cystic fibrosis, lithium degradation, acidic exopolysaccharide, mucoid, heptasaccharide
NCBI PubMed ID: 10095771Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: cerant@ipbs.fr
Institutions: Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France
Methods: methylation, NMR-2D, NMR, sugar analysis, carboxyl reduction, b-elimination, Smith degradation, de-O-acetylation, Li/ethylenediamine degradation, partial depolymerization
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3. Compound ID: 3142
Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_141806,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 1146
Robijn GW, van den Berg DJC, Haas H, Kamerling JP, Vliegenthart JFG "Determination of the structure of the exopolysaccharide produced by Lactobacillus sake 0-1" -
Carbohydrate Research 276(1) (1995) 117-136
The exopolysaccharide produced by Lactobacillus sake 0-1 in a semi-defined medium was found to have an average molecular mass of 6 x 10(6) Da and a composition of D-glucose, L-rhamnose, and sn-glycerol 3-phosphate (3:2:1). The polysaccharide is partially O-acetylated. By means of partial acid hydrolysis, O-deacetylation, deglycerophosphorylation, methylation analysis, and 1D/2D NMR (1H, 13C, and 31P) studies the polysaccharide was shown to be composed of repeating units with the following structure: [structure]
NMR, structure, polysaccharide, determination, Lactic acid bacteria, exopolysaccharide, Lactobacillus, L-rhamnose, O-acetyl, D-glucose, milk, Lactobacillus sakei, hosphoglycerol
NCBI PubMed ID: 8536250Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Bijvoet Center, Department of Bio-Organic Chemistry, Utrecht University, Utrecht, The Netherlands, Unilever Research Laboratory Vlaardingen, P.O. Box 114, NL-3130 AC, Vlaardingen, The Netherlands
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, FAB-MS, partial acid hydrolysis, sugar analysis, dephosphorylation, GLC, anion-exchange chromatography, de-O-acetylation
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4. Compound ID: 3248
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b-D-GlcpA-(1-2)-+
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b-D-Glcp-(1-4)-b-D-Manp-(1-4)-b-D-Glcp-(1-4)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_137485,IEDB_140630,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
The structure is contained in the following publication(s):
- Article ID: 1187
Senchenkova SN, Knirel YA, Likhosherstov LM, Shashkov AS, Shibaev VN, Starukhina LA, Deryabin VV "Structure of simusan, a new acidic exopolysaccharide from Arthrobacter sp" -
Carbohydrate Research 266 (1995) 103-113
Simusan, a major exopolysaccharide produced by an ethanol-utilizing Arthrobacter sp. strain CE-17, contains D-glucose, D-mannose, D-galactose, L-rhamnose, D-glucuronic acid, and pyruvic acid in the ratios approximately 3:2:1:1:1:1 as well as O-acyl groups (presumably (presumably residues of acetic and palmitic acid). On the basis of chemical modifications of the polysaccharide, solvolysis with anhydrous hydrogen fluoride resulting in a penta- and an octa-saccharide fragment, Smith degradation, and 1H and 13C NMR analysis, the following structure of the repeating unit was established: [formula: see text] It is suggested that at least one of the glucose residues and the galactose residue are O-acetylated.
structure, acidic, exopolysaccharide, extracellular, 2D NMR, 1H NMR, acidic exopolysaccharide, solvolysis, Arthrobacter, HF
NCBI PubMed ID: 7697646Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: sen@ioc.ac.ru
Institutions: N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow,Russia
Methods: NMR
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5. Compound ID: 3249
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b-D-GlcpA-(1-2)-+
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b-D-Manp-(1-6)-b-D-Glcp-(1-4)-a-D-Galp-(1-4)-b-D-Glcp-(1-4)-b-D-Manp-(1-4)-b-D-Glcp-(1-4)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_423153,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 1187
Senchenkova SN, Knirel YA, Likhosherstov LM, Shashkov AS, Shibaev VN, Starukhina LA, Deryabin VV "Structure of simusan, a new acidic exopolysaccharide from Arthrobacter sp" -
Carbohydrate Research 266 (1995) 103-113
Simusan, a major exopolysaccharide produced by an ethanol-utilizing Arthrobacter sp. strain CE-17, contains D-glucose, D-mannose, D-galactose, L-rhamnose, D-glucuronic acid, and pyruvic acid in the ratios approximately 3:2:1:1:1:1 as well as O-acyl groups (presumably (presumably residues of acetic and palmitic acid). On the basis of chemical modifications of the polysaccharide, solvolysis with anhydrous hydrogen fluoride resulting in a penta- and an octa-saccharide fragment, Smith degradation, and 1H and 13C NMR analysis, the following structure of the repeating unit was established: [formula: see text] It is suggested that at least one of the glucose residues and the galactose residue are O-acetylated.
structure, acidic, exopolysaccharide, extracellular, 2D NMR, 1H NMR, acidic exopolysaccharide, solvolysis, Arthrobacter, HF
NCBI PubMed ID: 7697646Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: sen@ioc.ac.ru
Institutions: N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow,Russia
Methods: NMR
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6. Compound ID: 3530
Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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7. Compound ID: 3531
Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_133754,IEDB_136105,IEDB_140630,IEDB_225177,IEDB_423153,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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8. Compound ID: 3535
|
a-L-Rhap-(1-2)-a-L-Rhap-(1-3)-b-D-Galp-(1-2)-a-L-Rhap-(1-4)-b-D-GlcpA-(1-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-2)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_133754,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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9. Compound ID: 3536
|
b-D-Galp-(1-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-4)-b-D-GlcpA-(1-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-3)-b-D-Galp-(1-2)-L-Rha-ol |
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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_133754,IEDB_136044,IEDB_136105,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 1305
Yun Yang B, Gray JSS, Montgomery R "Extracellular polysaccharide of Erwinia chrysanthemi CU643" -
Carbohydrate Research 316(1-4) (1999) 138-154
Erwinia chrysanthemi are gram-negative bacterial phytopathogens causing soft rots in a number of plants. The structure of the extracellular polysaccharide (EPS) produced by E. chrysanthemi strain CU643, pathogenic to Philodendron, has been determined using a combination of chemical and physical techniques including methylation analysis, high- and low-pressure gel-filtration and anion-exchange chromatography, high-pH anion-exchange chromatography, partial acid hydrolysis, mass spectrometry, and 1- and 2-D NMR spectroscopy. In contrast to the structures of the EPS reported for other strains of E. chrysanthemi, the EPS from strain CU643 is a linear polysaccharide containing L-Rhap, D-Galp, and D-GlcAp in the ratio 4:1:1. Evidence is presented for the following hexasaccharide repeat unit [see text]
structure, extracellular polysaccharide, Erwinia chrysanthemi, Philodendron
NCBI PubMed ID: 10420593Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: rex-montgomery@uiowa.edu
Institutions: Department of Biochemistry, College of Medicine, University of Iowa, Iowa City 52242, USA
Methods: methylation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, MALDI-TOF MS
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10. Compound ID: 5012
Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,SB_44,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 1961
Erbing C, Kenne L, Lindberg B, Hammarstrom S "Structure of the O-specific side-chains of the Escherichia coli O75 lipopolysaccharide: a revision" -
Carbohydrate Research 60(2) (1978) 400-403
no abstract available
Publication DOI: 10.1016/S0008-6215(78)80049-4Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Stockholm, Sweden, Department of Immunology, University of Stockholm, Stockholm, Sweden
Methods: 1H NMR, methylation, gel filtration, sugar analysis, acid hydrolysis, GLC, de-N-acetylation/deamination, ion-exchange chromatography, optical rotation measurement
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11. Compound ID: 5255
Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_137485,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
The structure is contained in the following publication(s):
- Article ID: 2163
Osman SF, Fett WF "The structure of the acidic exopolysaccharide of Pseudomonas marginalis strains PF-05-2 and PM-LB-1" -
Carbohydrate Research 199 (1990) 77-82
The structure of an acidic exopolysaccharide of two strains of Pseudomonas marginalis, a bacterium which causes soft rots of various vegetables, has been determined to consist of a repeating unit of: →4) β-d-Manp-(1→3)a-d-Glcp-(1→4)a-l-Rhap-(1-. The glucose is pyruvated at O-4 and O-6 and the mannose is acetylated at either O-2 or O-3.
Publication DOI: 10.1016/0008-6215(90)84094-BJournal NLM ID: 0043535Publisher: Elsevier
Institutions: U.S. Department of Agriculture, ARS, North Atlantic Area, 600 East Mermaid Lane, Philadelphia, PA 19118, U.S.A.
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12. Compound ID: 5256
|
b-D-Manp-(1-3)-a-D-Glcp-(1-4)-a-L-Rhap-(1-4)-b-D-Manp-(1-3)-a-D-Glcp-(1-4)-D-Rha-ol |
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Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_136105,IEDB_137485,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_225177,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
The structure is contained in the following publication(s):
- Article ID: 2163
Osman SF, Fett WF "The structure of the acidic exopolysaccharide of Pseudomonas marginalis strains PF-05-2 and PM-LB-1" -
Carbohydrate Research 199 (1990) 77-82
The structure of an acidic exopolysaccharide of two strains of Pseudomonas marginalis, a bacterium which causes soft rots of various vegetables, has been determined to consist of a repeating unit of: →4) β-d-Manp-(1→3)a-d-Glcp-(1→4)a-l-Rhap-(1-. The glucose is pyruvated at O-4 and O-6 and the mannose is acetylated at either O-2 or O-3.
Publication DOI: 10.1016/0008-6215(90)84094-BJournal NLM ID: 0043535Publisher: Elsevier
Institutions: U.S. Department of Agriculture, ARS, North Atlantic Area, 600 East Mermaid Lane, Philadelphia, PA 19118, U.S.A.
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13. Compound ID: 5265
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a-L-Rhap-(1-3)-b-D-Glcp-(1-2)-+
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b-D-Glcp-(1-2)-+ |
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b-D-GlcpA-(1-3)-a-L-Rhap-(1-3)-a-L-Rhap-(1-2)-Rha-ol |
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Structure type: oligomer
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_136105,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_189517,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 2166
Jackson GE, Ravenscroft N, Stephen AM "The use of bacteriophage-mediated depolymerization in investigations of the structure of the capsular polysaccharide from Klebsiella serotype K71" -
Carbohydrate Research 200(1) (1990) 409-428
The structure (1) of the heptasaccharide repeating-unit of the capsular polysaccharide from Klebsiella serotype K71 follows from methylation analysis and n.m.r. and mass-spectrometric studies of the oligosaccharides obtained on depolymerisation of the polysaccharide with a bacteriophage-borne endorhamnosidase [structure: see text].
Publication DOI: 10.1016/0008-6215(90)84207-BJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Chemistry, University of Cape Town, Rondebosch 7700 Republic of South Africa
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, NaBH4 reduction, SEC, phage degradation
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14. Compound ID: 5413
Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 2261
Gray JSS, Brand JM, Koerner TAW, Montgomery R "Structure of an extracellular polysaccharide produced by Erwinia chrysanthemi" -
Carbohydrate Research 245 (1993) 271-287
Erwinia chrysanthemi pv zeae strain SR260, a phytopathogen of corn, produced from lactose an acidic extracellular polysaccharide which was purified and found to consist of L-rhamnose, D-mannose, D-glucose, and D-glucuronic acid in the ratio of 3:1:1:1. A combination of chemical (carboxyl-group reduction, methylation analysis, periodate oxidation, Smith degradation, and lithium-ethylenediamine degradation) and physical (1 and 2D NMR spectroscopy) methods revealed that the polysaccharide is composed of a hexasaccharide repeating unit 1: [formula: see text]
NCBI PubMed ID: 8370026Publication DOI: 10.1016/0008-6215(93)80077-RJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Biochemistry, University of Fort Hare, Alice, South Africa
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, NMR-2D, carboxyl reduction, Smith degradation, Li/ethylenediamine degradation
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15. Compound ID: 5935
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b-D-Glcp-(1-3)-+
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a-D-Manp-(1-2)-b-D-Manp-(1-3)-b-D-Manp-(1-3)-L-Rha-ol |
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Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137485,IEDB_140116,IEDB_142488,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_136,SB_192,SB_196,SB_44,SB_67,SB_72
The structure is contained in the following publication(s):
- Article ID: 2641
Kocharova NA, Knirel YA, Shashkov AS, Kochetkov NK, Pier GB "Structure of an extracellular cross-reactive polysaccharide from Pseudomonas aeruginosa immunotype 4" -
Journal of Biological Chemistry 263 (1988) 11291-11295
A neutral small molecular mass (approximately 6.5 kDa) polysaccharide comprising a pentasaccharide repeat unit was isolated from culture supernatants of Pseudomonas aeruginosa immunotype 4. The polysaccharide had a pentasaccharide repeating unit as follows (formula; see text) where Rha is rhamnose. The structure was determined using acid hydrolysis, solvolysis with anhydrous hydrogen fluoride, methylation analysis, and 1H and 13C nuclear magnetic resonance spectroscopy including nuclear Overhauser enhancement experiments. The polysaccharide bound antibody raised to the lipopolysaccharide of the seven P. aeruginosa Fisher-Devlin immunotype strains. Inhibition assays demonstrated the presence of a serologically similar polysaccharide in supernatants of these strains. Affinity-purified antibody to the polysaccharide bound to lipopolysaccharide and whole cells of the immunotype strains of P. aeruginosa in a Western immunoblot and colony blot assay, respectively. This polysaccharide seems to contain an antigenic determinant present in the core of the P. aeruginosa lipopolysaccharide or may represent another minor polysaccharide substituent on the lipopolysaccharide in addition to the O side chain.
NCBI PubMed ID: 3136157Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the Union of Soviet Socialist Republics, Moscow, Chunning Laboratory, Department of Medicine, Brighum and Women’s Hospital, Harvard Medical School, Boston. Massachusetts 02115
Methods: 13C NMR, 1H NMR
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Next 15 structure(s)
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Total list of corresponding CSDB IDs (permanent record IDs):
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