The structure of the exopolysaccharide (EPS) produced by a clinical isolate of Burkholderia cepacia isolated from a patient with fibrocystic lung disease has been investigated. By means of methylation analyses, carboxyl reduction, partial depolymerization by fuming HCl and chemical degradations such as Smith degradation, lithiumethylenediamine degradation and beta-elimination, supported by GC/MS and NMR spectroscopic analyses, the repeat unit of the EPS has been identified and was shown to correspond to the acidic branched heptasaccharide with the following structure: [formula: see text]. This partially acetylated acidic polymer, distinguished by the presence of the less usual D-isomer of rhamnose and of a trisubstituted glucuronic acid residue, could represent the main EPS produced by this bacterial species.
clinical, isolate, structural, characterization, Burkholderia, Burkholderia cepacia, repeating unit, acid, NMR spectroscopy, exopolysaccharide, elucidation, heptasaccharide repeating unit, glucuronic acid, cystic fibrosis, lithium degradation, acidic exopolysaccharide, mucoid, heptasaccharide
NCBI PubMed ID: 10095771Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: cerant@ipbs.fr
Institutions: Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France
Methods: methylation, NMR-2D, NMR, sugar analysis, carboxyl reduction, b-elimination, Smith degradation, de-O-acetylation, Li/ethylenediamine degradation, partial depolymerization
Found