Found 11 structures.
Displayed structures from 1 to 11
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1. Compound ID: 2282
Structure type: oligomer
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
The structure is contained in the following publication(s):
- Article ID: 765
Cérantola S, Lemassu-Jacquier A, Montrozier H "Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of Burkholderia cepacia - Characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit" -
European Journal of Biochemistry 260(2) (1999) 373-383
The structure of the exopolysaccharide (EPS) produced by a clinical isolate of Burkholderia cepacia isolated from a patient with fibrocystic lung disease has been investigated. By means of methylation analyses, carboxyl reduction, partial depolymerization by fuming HCl and chemical degradations such as Smith degradation, lithiumethylenediamine degradation and beta-elimination, supported by GC/MS and NMR spectroscopic analyses, the repeat unit of the EPS has been identified and was shown to correspond to the acidic branched heptasaccharide with the following structure: [formula: see text]. This partially acetylated acidic polymer, distinguished by the presence of the less usual D-isomer of rhamnose and of a trisubstituted glucuronic acid residue, could represent the main EPS produced by this bacterial species.
clinical, isolate, structural, characterization, Burkholderia, Burkholderia cepacia, repeating unit, acid, NMR spectroscopy, exopolysaccharide, elucidation, heptasaccharide repeating unit, glucuronic acid, cystic fibrosis, lithium degradation, acidic exopolysaccharide, mucoid, heptasaccharide
NCBI PubMed ID: 10095771Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: cerant@ipbs.fr
Institutions: Institut de Pharmacologie et de Biologie Structurale du CNRS, Toulouse, France
Methods: methylation, NMR-2D, NMR, sugar analysis, carboxyl reduction, b-elimination, Smith degradation, de-O-acetylation, Li/ethylenediamine degradation, partial depolymerization
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2. Compound ID: 5255
Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_137485,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
The structure is contained in the following publication(s):
- Article ID: 2163
Osman SF, Fett WF "The structure of the acidic exopolysaccharide of Pseudomonas marginalis strains PF-05-2 and PM-LB-1" -
Carbohydrate Research 199 (1990) 77-82
The structure of an acidic exopolysaccharide of two strains of Pseudomonas marginalis, a bacterium which causes soft rots of various vegetables, has been determined to consist of a repeating unit of: →4) β-d-Manp-(1→3)a-d-Glcp-(1→4)a-l-Rhap-(1-. The glucose is pyruvated at O-4 and O-6 and the mannose is acetylated at either O-2 or O-3.
Publication DOI: 10.1016/0008-6215(90)84094-BJournal NLM ID: 0043535Publisher: Elsevier
Institutions: U.S. Department of Agriculture, ARS, North Atlantic Area, 600 East Mermaid Lane, Philadelphia, PA 19118, U.S.A.
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3. Compound ID: 5256
|
b-D-Manp-(1-3)-a-D-Glcp-(1-4)-a-L-Rhap-(1-4)-b-D-Manp-(1-3)-a-D-Glcp-(1-4)-D-Rha-ol |
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Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_136105,IEDB_137485,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_225177,IEDB_885823,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72
The structure is contained in the following publication(s):
- Article ID: 2163
Osman SF, Fett WF "The structure of the acidic exopolysaccharide of Pseudomonas marginalis strains PF-05-2 and PM-LB-1" -
Carbohydrate Research 199 (1990) 77-82
The structure of an acidic exopolysaccharide of two strains of Pseudomonas marginalis, a bacterium which causes soft rots of various vegetables, has been determined to consist of a repeating unit of: →4) β-d-Manp-(1→3)a-d-Glcp-(1→4)a-l-Rhap-(1-. The glucose is pyruvated at O-4 and O-6 and the mannose is acetylated at either O-2 or O-3.
Publication DOI: 10.1016/0008-6215(90)84094-BJournal NLM ID: 0043535Publisher: Elsevier
Institutions: U.S. Department of Agriculture, ARS, North Atlantic Area, 600 East Mermaid Lane, Philadelphia, PA 19118, U.S.A.
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4. Compound ID: 5265
|
a-L-Rhap-(1-3)-b-D-Glcp-(1-2)-+
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b-D-Glcp-(1-2)-+ |
| |
b-D-GlcpA-(1-3)-a-L-Rhap-(1-3)-a-L-Rhap-(1-2)-Rha-ol |
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Structure type: oligomer
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_136105,IEDB_140630,IEDB_142488,IEDB_146664,IEDB_189517,IEDB_225177,IEDB_423153,IEDB_885823,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 2166
Jackson GE, Ravenscroft N, Stephen AM "The use of bacteriophage-mediated depolymerization in investigations of the structure of the capsular polysaccharide from Klebsiella serotype K71" -
Carbohydrate Research 200(1) (1990) 409-428
The structure (1) of the heptasaccharide repeating-unit of the capsular polysaccharide from Klebsiella serotype K71 follows from methylation analysis and n.m.r. and mass-spectrometric studies of the oligosaccharides obtained on depolymerisation of the polysaccharide with a bacteriophage-borne endorhamnosidase [structure: see text].
Publication DOI: 10.1016/0008-6215(90)84207-BJournal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Chemistry, University of Cape Town, Rondebosch 7700 Republic of South Africa
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, NMR-2D, sugar analysis, NaBH4 reduction, SEC, phage degradation
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5. Compound ID: 25265
Structure type: oligomer
Contained glycoepitopes: IEDB_136907,IEDB_581506
The structure is contained in the following publication(s):
- Article ID: 10373
Lau JM, McNeil M, Darvill AG, Albersheim P "Treatment of rhamnogalacturonan I with lithium in ethylenediamine" -
Carbohydrate Research 168 (1987) 245-274
Rhamnogalacturonan I is a pectic polysaccharide that is solubilized from the walls of suspension-cultured sycamore cells (Acer pseudoplatanus) by the action of a highly purified endo-1,4-α-polygalacturonanase. Rhamnogalacturonan I has a linear backbone consisting of the diglycosyl repeating unit, →4)-α-d-GalpA-(1→2)-α-l-Rhap-(1→. Approximately half of the α-l-rhamnosyl residues of the backbone are branched at O-4. Selective cleavage at the galactosyluronic acid residues of the backbone by treatment of rhamnogalacturonan I wit lithium in ethylenediamine resulted in the release of the neutral glycosyl-residue sidechains that had been attached to the backbone. Various analytical techniques, including combined liquid chromatography-mass spectrometry, combined gas-liquid chromatography-mass spectrometry, and 1H-nuclear magnetic resonance spectroscopy, were used to determine the structure of the side chains. The majority of the sidechains were isolated as oligoglycosylalditols, with rhamnitol at the “reducing” end. Terminal 2-, 4-, or 6-linked galactosyl residues were found attached to O-4 of the rhamnitol residues The 2-, 4-, and 6-linked galactosyl residues had terminal or 2-linked arabinosyl, or additional galactosyl, residues attached to them. Based on the results of fast-atom-bombardment mass spectrometry, the side chains were found to range in size from one to fourteen glycosyl residues. The side-chain structures suggest that there are four or more distinct families of side chains attached to the backbone of rhamnogalacturonan I.
Publication DOI: 10.1016/0008-6215(87)80029-0Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Chemistry, University of Colorado, Boulder, and Complex Carbohydrate Research Center and School of Chemical Sciences, University of Georgia, Athens, GA, U.S.A.
Methods: 1H NMR, GLC-MS, gel filtration, FAB-MS
- Article ID: 10468
Hirano M, Kiyohara H, Matsumoto T, Yamada H "Studies on antiulcer pectic polysaccharides from Bupleurum falcatum, Part V. Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L." -
Carbohydrate Research 251 (1994) 145-162
The endoα-(1→4)-polygalacturonase-resistant fractions (PG-1, PG-2, and PG-3) from an antiulcer pectin (Bupleuran 2IIc), isolated from the roots of Bupleurum falcatum, L., were further analysed by lithium degradation. The results indicated that PG-1 contained a small proportion of long, branched arabinosyl chains and a large proportion of short, neutral oligosaccharide chains. GLC-MS analysis showed that, after methylation the short, neutral oligosaccharide fraction consisted of at least 22 kinds of di- to tetra-saccharide alditols, such as Gal-(1→4)-Rha-ol (a major component), Ara-(1→4)-Rha-ol, Glc-(1→4)-Rha-ol, Ara→Ara→Ara-ol, and Ara→Ara→Ara→Ara-ol (minor components) in addition to heteroglycosyl alditols. After deesterification, PG-2 and PG-3 were digested with endo-α-(1→4)-polygalacturonase again, and the enzyme-resistant intermediate size fraction (PG-2′) was purified. Component sugar analysis indicated that PG-2′ contained 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), 3-deoxy-d-lyxo-heptulosaric acid (Dha), and 3-deoxy-d-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of PG-2′ gave mainly a pentosyl→6-deoxyhexosyl→6-deoxyhexosyl→pentitol fragment, with some neutral di- and tri-saccharide alditols, including a pentosysl→deoxyhexitol. Methylation analysis of these degradation products indicated that they contained terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, 3-linked Ara, and 3′-linked Api. Bupleuran 2IIc was eluted as essentially a single peak on gel filtration on Sepharose CL-6B. The neutral sugar content of the successive fractions increased with increasing molecular weight, but each fraction also contained, in addition to Rha, Ara, and Gal, 2-Me-Fuc, 2-Me-Xyl, and Api.
NCBI PubMed ID: 8149369Publication DOI: 10.1016/0008-6215(94)84282-5Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan
Methods: EI-MS, gel filtration, GLC-EI-MS, GLC, HPLC, CI-MS, methylation analysis
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6. Compound ID: 25268
Structure type: oligomer
Contained glycoepitopes: IEDB_136907,IEDB_581506
The structure is contained in the following publication(s):
- Article ID: 10373
Lau JM, McNeil M, Darvill AG, Albersheim P "Treatment of rhamnogalacturonan I with lithium in ethylenediamine" -
Carbohydrate Research 168 (1987) 245-274
Rhamnogalacturonan I is a pectic polysaccharide that is solubilized from the walls of suspension-cultured sycamore cells (Acer pseudoplatanus) by the action of a highly purified endo-1,4-α-polygalacturonanase. Rhamnogalacturonan I has a linear backbone consisting of the diglycosyl repeating unit, →4)-α-d-GalpA-(1→2)-α-l-Rhap-(1→. Approximately half of the α-l-rhamnosyl residues of the backbone are branched at O-4. Selective cleavage at the galactosyluronic acid residues of the backbone by treatment of rhamnogalacturonan I wit lithium in ethylenediamine resulted in the release of the neutral glycosyl-residue sidechains that had been attached to the backbone. Various analytical techniques, including combined liquid chromatography-mass spectrometry, combined gas-liquid chromatography-mass spectrometry, and 1H-nuclear magnetic resonance spectroscopy, were used to determine the structure of the side chains. The majority of the sidechains were isolated as oligoglycosylalditols, with rhamnitol at the “reducing” end. Terminal 2-, 4-, or 6-linked galactosyl residues were found attached to O-4 of the rhamnitol residues The 2-, 4-, and 6-linked galactosyl residues had terminal or 2-linked arabinosyl, or additional galactosyl, residues attached to them. Based on the results of fast-atom-bombardment mass spectrometry, the side chains were found to range in size from one to fourteen glycosyl residues. The side-chain structures suggest that there are four or more distinct families of side chains attached to the backbone of rhamnogalacturonan I.
Publication DOI: 10.1016/0008-6215(87)80029-0Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Chemistry, University of Colorado, Boulder, and Complex Carbohydrate Research Center and School of Chemical Sciences, University of Georgia, Athens, GA, U.S.A.
Methods: 1H NMR, GLC-MS, gel filtration, FAB-MS
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7. Compound ID: 25269
Structure type: oligomer
Contained glycoepitopes: IEDB_115015,IEDB_136045,IEDB_136907,IEDB_142489,IEDB_144562,IEDB_149135,IEDB_152214,IEDB_174333,IEDB_581506,SB_86
The structure is contained in the following publication(s):
- Article ID: 10373
Lau JM, McNeil M, Darvill AG, Albersheim P "Treatment of rhamnogalacturonan I with lithium in ethylenediamine" -
Carbohydrate Research 168 (1987) 245-274
Rhamnogalacturonan I is a pectic polysaccharide that is solubilized from the walls of suspension-cultured sycamore cells (Acer pseudoplatanus) by the action of a highly purified endo-1,4-α-polygalacturonanase. Rhamnogalacturonan I has a linear backbone consisting of the diglycosyl repeating unit, →4)-α-d-GalpA-(1→2)-α-l-Rhap-(1→. Approximately half of the α-l-rhamnosyl residues of the backbone are branched at O-4. Selective cleavage at the galactosyluronic acid residues of the backbone by treatment of rhamnogalacturonan I wit lithium in ethylenediamine resulted in the release of the neutral glycosyl-residue sidechains that had been attached to the backbone. Various analytical techniques, including combined liquid chromatography-mass spectrometry, combined gas-liquid chromatography-mass spectrometry, and 1H-nuclear magnetic resonance spectroscopy, were used to determine the structure of the side chains. The majority of the sidechains were isolated as oligoglycosylalditols, with rhamnitol at the “reducing” end. Terminal 2-, 4-, or 6-linked galactosyl residues were found attached to O-4 of the rhamnitol residues The 2-, 4-, and 6-linked galactosyl residues had terminal or 2-linked arabinosyl, or additional galactosyl, residues attached to them. Based on the results of fast-atom-bombardment mass spectrometry, the side chains were found to range in size from one to fourteen glycosyl residues. The side-chain structures suggest that there are four or more distinct families of side chains attached to the backbone of rhamnogalacturonan I.
Publication DOI: 10.1016/0008-6215(87)80029-0Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Chemistry, University of Colorado, Boulder, and Complex Carbohydrate Research Center and School of Chemical Sciences, University of Georgia, Athens, GA, U.S.A.
Methods: 1H NMR, GLC-MS, gel filtration, FAB-MS
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8. Compound ID: 25272
Structure type: oligomer
Contained glycoepitopes: IEDB_115013,IEDB_116886,IEDB_130645,IEDB_130651,IEDB_131186,IEDB_134624,IEDB_135818,IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141492,IEDB_141794,IEDB_144987,IEDB_149137,IEDB_149176,IEDB_149558,IEDB_151528,IEDB_153201,IEDB_156489,IEDB_156493,IEDB_156494,IEDB_156557,IEDB_156983,IEDB_167072,IEDB_190606,IEDB_221845,IEDB_241097,IEDB_742245,IEDB_742246,IEDB_742247,IEDB_742248,IEDB_885812,IEDB_918313,IEDB_918314,SB_163,SB_165,SB_166,SB_187,SB_195,SB_31,SB_62,SB_7,SB_87,SB_88
The structure is contained in the following publication(s):
- Article ID: 10373
Lau JM, McNeil M, Darvill AG, Albersheim P "Treatment of rhamnogalacturonan I with lithium in ethylenediamine" -
Carbohydrate Research 168 (1987) 245-274
Rhamnogalacturonan I is a pectic polysaccharide that is solubilized from the walls of suspension-cultured sycamore cells (Acer pseudoplatanus) by the action of a highly purified endo-1,4-α-polygalacturonanase. Rhamnogalacturonan I has a linear backbone consisting of the diglycosyl repeating unit, →4)-α-d-GalpA-(1→2)-α-l-Rhap-(1→. Approximately half of the α-l-rhamnosyl residues of the backbone are branched at O-4. Selective cleavage at the galactosyluronic acid residues of the backbone by treatment of rhamnogalacturonan I wit lithium in ethylenediamine resulted in the release of the neutral glycosyl-residue sidechains that had been attached to the backbone. Various analytical techniques, including combined liquid chromatography-mass spectrometry, combined gas-liquid chromatography-mass spectrometry, and 1H-nuclear magnetic resonance spectroscopy, were used to determine the structure of the side chains. The majority of the sidechains were isolated as oligoglycosylalditols, with rhamnitol at the “reducing” end. Terminal 2-, 4-, or 6-linked galactosyl residues were found attached to O-4 of the rhamnitol residues The 2-, 4-, and 6-linked galactosyl residues had terminal or 2-linked arabinosyl, or additional galactosyl, residues attached to them. Based on the results of fast-atom-bombardment mass spectrometry, the side chains were found to range in size from one to fourteen glycosyl residues. The side-chain structures suggest that there are four or more distinct families of side chains attached to the backbone of rhamnogalacturonan I.
Publication DOI: 10.1016/0008-6215(87)80029-0Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Chemistry, University of Colorado, Boulder, and Complex Carbohydrate Research Center and School of Chemical Sciences, University of Georgia, Athens, GA, U.S.A.
Methods: 1H NMR, GLC-MS, gel filtration, FAB-MS
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9. Compound ID: 25696
Structure type: oligomer
Contained glycoepitopes: IEDB_136105,IEDB_1394181,IEDB_141798,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 10468
Hirano M, Kiyohara H, Matsumoto T, Yamada H "Studies on antiulcer pectic polysaccharides from Bupleurum falcatum, Part V. Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L." -
Carbohydrate Research 251 (1994) 145-162
The endoα-(1→4)-polygalacturonase-resistant fractions (PG-1, PG-2, and PG-3) from an antiulcer pectin (Bupleuran 2IIc), isolated from the roots of Bupleurum falcatum, L., were further analysed by lithium degradation. The results indicated that PG-1 contained a small proportion of long, branched arabinosyl chains and a large proportion of short, neutral oligosaccharide chains. GLC-MS analysis showed that, after methylation the short, neutral oligosaccharide fraction consisted of at least 22 kinds of di- to tetra-saccharide alditols, such as Gal-(1→4)-Rha-ol (a major component), Ara-(1→4)-Rha-ol, Glc-(1→4)-Rha-ol, Ara→Ara→Ara-ol, and Ara→Ara→Ara→Ara-ol (minor components) in addition to heteroglycosyl alditols. After deesterification, PG-2 and PG-3 were digested with endo-α-(1→4)-polygalacturonase again, and the enzyme-resistant intermediate size fraction (PG-2′) was purified. Component sugar analysis indicated that PG-2′ contained 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), 3-deoxy-d-lyxo-heptulosaric acid (Dha), and 3-deoxy-d-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of PG-2′ gave mainly a pentosyl→6-deoxyhexosyl→6-deoxyhexosyl→pentitol fragment, with some neutral di- and tri-saccharide alditols, including a pentosysl→deoxyhexitol. Methylation analysis of these degradation products indicated that they contained terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, 3-linked Ara, and 3′-linked Api. Bupleuran 2IIc was eluted as essentially a single peak on gel filtration on Sepharose CL-6B. The neutral sugar content of the successive fractions increased with increasing molecular weight, but each fraction also contained, in addition to Rha, Ara, and Gal, 2-Me-Fuc, 2-Me-Xyl, and Api.
NCBI PubMed ID: 8149369Publication DOI: 10.1016/0008-6215(94)84282-5Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan
Methods: EI-MS, gel filtration, GLC-EI-MS, GLC, HPLC, CI-MS, methylation analysis
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10. Compound ID: 25697
Structure type: oligomer
Contained glycoepitopes: IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_165,SB_166,SB_187,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 10468
Hirano M, Kiyohara H, Matsumoto T, Yamada H "Studies on antiulcer pectic polysaccharides from Bupleurum falcatum, Part V. Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L." -
Carbohydrate Research 251 (1994) 145-162
The endoα-(1→4)-polygalacturonase-resistant fractions (PG-1, PG-2, and PG-3) from an antiulcer pectin (Bupleuran 2IIc), isolated from the roots of Bupleurum falcatum, L., were further analysed by lithium degradation. The results indicated that PG-1 contained a small proportion of long, branched arabinosyl chains and a large proportion of short, neutral oligosaccharide chains. GLC-MS analysis showed that, after methylation the short, neutral oligosaccharide fraction consisted of at least 22 kinds of di- to tetra-saccharide alditols, such as Gal-(1→4)-Rha-ol (a major component), Ara-(1→4)-Rha-ol, Glc-(1→4)-Rha-ol, Ara→Ara→Ara-ol, and Ara→Ara→Ara→Ara-ol (minor components) in addition to heteroglycosyl alditols. After deesterification, PG-2 and PG-3 were digested with endo-α-(1→4)-polygalacturonase again, and the enzyme-resistant intermediate size fraction (PG-2′) was purified. Component sugar analysis indicated that PG-2′ contained 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), 3-deoxy-d-lyxo-heptulosaric acid (Dha), and 3-deoxy-d-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of PG-2′ gave mainly a pentosyl→6-deoxyhexosyl→6-deoxyhexosyl→pentitol fragment, with some neutral di- and tri-saccharide alditols, including a pentosysl→deoxyhexitol. Methylation analysis of these degradation products indicated that they contained terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, 3-linked Ara, and 3′-linked Api. Bupleuran 2IIc was eluted as essentially a single peak on gel filtration on Sepharose CL-6B. The neutral sugar content of the successive fractions increased with increasing molecular weight, but each fraction also contained, in addition to Rha, Ara, and Gal, 2-Me-Fuc, 2-Me-Xyl, and Api.
NCBI PubMed ID: 8149369Publication DOI: 10.1016/0008-6215(94)84282-5Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan
Methods: EI-MS, gel filtration, GLC-EI-MS, GLC, HPLC, CI-MS, methylation analysis
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11. Compound ID: 25698
Structure type: oligomer
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_983931,SB_192
The structure is contained in the following publication(s):
- Article ID: 10468
Hirano M, Kiyohara H, Matsumoto T, Yamada H "Studies on antiulcer pectic polysaccharides from Bupleurum falcatum, Part V. Structural studies of endopolygalacturonase-resistant fragments of an antiulcer pectin from the roots of Bupleurum falcatum L." -
Carbohydrate Research 251 (1994) 145-162
The endoα-(1→4)-polygalacturonase-resistant fractions (PG-1, PG-2, and PG-3) from an antiulcer pectin (Bupleuran 2IIc), isolated from the roots of Bupleurum falcatum, L., were further analysed by lithium degradation. The results indicated that PG-1 contained a small proportion of long, branched arabinosyl chains and a large proportion of short, neutral oligosaccharide chains. GLC-MS analysis showed that, after methylation the short, neutral oligosaccharide fraction consisted of at least 22 kinds of di- to tetra-saccharide alditols, such as Gal-(1→4)-Rha-ol (a major component), Ara-(1→4)-Rha-ol, Glc-(1→4)-Rha-ol, Ara→Ara→Ara-ol, and Ara→Ara→Ara→Ara-ol (minor components) in addition to heteroglycosyl alditols. After deesterification, PG-2 and PG-3 were digested with endo-α-(1→4)-polygalacturonase again, and the enzyme-resistant intermediate size fraction (PG-2′) was purified. Component sugar analysis indicated that PG-2′ contained 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), 3-deoxy-d-lyxo-heptulosaric acid (Dha), and 3-deoxy-d-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of PG-2′ gave mainly a pentosyl→6-deoxyhexosyl→6-deoxyhexosyl→pentitol fragment, with some neutral di- and tri-saccharide alditols, including a pentosysl→deoxyhexitol. Methylation analysis of these degradation products indicated that they contained terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, 3-linked Ara, and 3′-linked Api. Bupleuran 2IIc was eluted as essentially a single peak on gel filtration on Sepharose CL-6B. The neutral sugar content of the successive fractions increased with increasing molecular weight, but each fraction also contained, in addition to Rha, Ara, and Gal, 2-Me-Fuc, 2-Me-Xyl, and Api.
NCBI PubMed ID: 8149369Publication DOI: 10.1016/0008-6215(94)84282-5Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Oriental Medicine Research Center of the Kitasato Institute, Tokyo, Japan
Methods: EI-MS, gel filtration, GLC-EI-MS, GLC, HPLC, CI-MS, methylation analysis
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Total list of structure IDs on all result pages of the current query:
Total list of corresponding CSDB IDs (permanent record IDs):
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