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Structure type: oligomer
Contained glycoepitopes: IEDB_115136,IEDB_136044,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_190606,IEDB_423153,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88

• Article ID: 867
  Kocharova NA, Shcherbakova OV, Shashkov AS, Knirel YA, Kochetkov NK, Kholodkova EV, Stanislavsky ES "Structure of the O-specific polysaccharide of the bacterium Providencia alcalifaciens serogroup O23 containing a novel amide of D-glucuronic acid with Ne-(1-carboxyethyl)lysine" - Biochemistry (Moscow) 62 (1997) 501-508
  

  An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O23 and found to contain D-glucose, D-galactose, 2-acetamido-2-deoxy-D-galactose, and N ε-(1-carboxyethyl)-N α-(D-glucuronoyl)lysine. On the basis of full and partial acid hydrolyses, selective solvolysis with anhydrous hydrogen fluoride, and 1H- and 13C NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H, 13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), the following structure of the liner tetrasaccharide repeating unit of the polysaccharide was established [structure: see text]

  Lipopolysaccharide, NMR, LPS, structure, polysaccharide, O-antigen, acidic, acid, bacteria, O-specific, O-specific polysaccharide, Providencia, Providencia alcalifaciens, serogroup, component, amino acid, Alanine, Alaninolysine, D-glucuronic acid, amide, lysine

  NCBI PubMed ID: 9275290
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: knirel@ioc.ac.ru
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  Methods: partial acid hydrolysis, NMR, HF solvolysis, MS, borohydride reduction
  
   
  
  Providencia alcalifaciens O23
  CSDB ID 2961 (all data & tools)

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Structure type: monomer
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_423153

• Article ID: 867
  Kocharova NA, Shcherbakova OV, Shashkov AS, Knirel YA, Kochetkov NK, Kholodkova EV, Stanislavsky ES "Structure of the O-specific polysaccharide of the bacterium Providencia alcalifaciens serogroup O23 containing a novel amide of D-glucuronic acid with Ne-(1-carboxyethyl)lysine" - Biochemistry (Moscow) 62 (1997) 501-508
  

  An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Providencia alcalifaciens O23 and found to contain D-glucose, D-galactose, 2-acetamido-2-deoxy-D-galactose, and N ε-(1-carboxyethyl)-N α-(D-glucuronoyl)lysine. On the basis of full and partial acid hydrolyses, selective solvolysis with anhydrous hydrogen fluoride, and 1H- and 13C NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear 1H, 13C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), the following structure of the liner tetrasaccharide repeating unit of the polysaccharide was established [structure: see text]

  Lipopolysaccharide, NMR, LPS, structure, polysaccharide, O-antigen, acidic, acid, bacteria, O-specific, O-specific polysaccharide, Providencia, Providencia alcalifaciens, serogroup, component, amino acid, Alanine, Alaninolysine, D-glucuronic acid, amide, lysine

  NCBI PubMed ID: 9275290
  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: knirel@ioc.ac.ru
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  Methods: partial acid hydrolysis, NMR, HF solvolysis, MS, borohydride reduction
  
   
  
  Providencia alcalifaciens O23
  CSDB ID 3071 (all data & tools)

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Structure type: monomer
Contained glycoepitopes: IEDB_115136,IEDB_140630,IEDB_423153

• Article ID: 869
  Kocharova NA, Vinogradov EV, Borisova SA, Shashkov AS, Knirel YA "Identification of Ne-[(R)-1-carboxyethyl]-L-lysine in, and the complete structure of, the repeating unit of the O-specific polysaccharide of Providencia alcalifaciens O23" - Carbohydrate Research 309(1) (1998) 131-133
  

  N ε-[(R)-1-Carboxyethyl]-L-lysine was released by acid hydrolysis from the O-specific polysaccharide of Providencia alcalifaciens O23 and identified by 1H and 13C NMR spectroscopy, GLC-MS after conversion to a di-N-acetylated dimethyl ester, and by comparison with the authentic sample. Solvolysis of the polysaccharide with anhydrous HF resulted in an amide of D-glucuronic acid with N ε-[(R)-1-carboxyethyl]-L-lysine. These and published data allowed the determination of the full structure of the repeating unit of the O-specific polysaccharide.

  structure, polysaccharide, repeating unit, O-specific, O-specific polysaccharide, Providencia, Providencia alcalifaciens, identification, Alaninolysine

  NCBI PubMed ID: 9720244
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: knirel@ioc.ac.ru
  Institutions: N.D.Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  Methods: NMR, HF solvolysis, MS
  
   
  
  Providencia alcalifaciens O23
  CSDB ID 2969 (all data & tools)

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Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_135813,IEDB_137340,IEDB_137473,IEDB_1391961,IEDB_140630,IEDB_141584,IEDB_141807,IEDB_151531,IEDB_423153,IEDB_885822

• Article ID: 891
  Kondakova AN, Toukach FV, Senchenkova SN, Arbatsky NP, Shashkov AS, Knirel YA, Bartodziejska B, Zych K, Rozalski A, Sidorczyk Z "New structures of the O-specific polysaccharides of Proteus. Part 3. Polysaccharides containing non-sugar organic acids" - Biochemistry (Moscow) 68(4) (2003) 446-457
  

  structure, polysaccharide, O-antigen, acid, O-specific, O-specific polysaccharide, Proteus, polysaccharides, O-specific polysaccharides, amino, organic, organic acid, lactic

  Journal NLM ID: 0376536
  Publisher: Nauka/Interperiodica
  Correspondence: knirel@ioc.ac.ru
  Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  Methods: NMR, acid hydrolysis
  
   
  
  Proteus myxofaciens
  CSDB ID 2994 (all data & tools)

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Structure type: polymer chemical repeating unit
Contained glycoepitopes: IEDB_115136,IEDB_130648,IEDB_137473,IEDB_140630,IEDB_153510,IEDB_423153

• Article ID: 3724
  Kwinkowski M, Grabowski S, Konieczna I, Nazarenko EL, Kaca W "The cross-reactivity of Shewanella fidelis lipopolysaccharide with anti-Proteus antibodies" - Polish Journal of Microbiology 58(3) (2009) 275-278
  

  The serological cross-reactivity between lipopolysaccharides (LPS) of S. fidelis KMM3582(T) and rabbit anti-O P. mirabilis antibodies was tested. Using ELISA and Western blot cross-reactivity between S. fidelis LPS and antisera against P. mirabilis O14, O3 LPSs was found. The observed cross-reaction may suggest that anti-P. mirabilis S1959 (O3) antibodies may bind to the internal part of S. fidelis O-polysaccharides. A weak interaction between S. fidelis LPS and antiserum against P. mirabilis O13 in Western blot suggests that the absolute configuration of non-sugar 'AlaLys' component (N(ε)-[(S)-l-carboxyethyl]-N(α)-(D-galacturonoyl)-L-lysine) may influence the affinity of antibodies for S. fidelis LPS.

  lipopolysaccharides, Proteus mirabilis, cross-reactivity, Shewanella fidelis

  NCBI PubMed ID: 19899622
  Journal NLM ID: 101229003
  Publisher: Polskie Towarzystwo Mikrobiologow
  Correspondence: marek.kwinkowski@ujk.edu.pl
  Institutions: Department of Microbiology, Institute of Biology, Jan Kochanowski University, Kielce, Poland
  Methods: ELISA, serological methods
  
   
  
  Proteus mirabilis O13
  CSDB ID 24064 (all data & tools)