Show legend Show as text

Structure type: oligomer
Compound class: lipid A
Contained glycoepitopes: IEDB_136907,IEDB_141807,IEDB_151531,IEDB_534864,IEDB_581506

• Article ID: 99
  Therisod H, Karibian D, Perry MB, Caroff M "Structural analysis of Yersinia pseudotuberculosis ATCC 29833 lipid A" - International Journal of Mass Spectrometry 219(3) (2002) 549-557
  

  The Yersinia genus includes human and animal pathogens (plague, enterocolitis) as well as non-pathogens. The lipopolysaccharide of the facultative pathogen Yersinia pseudotuberculosis has been implicated in the invasiveness of these bacteria. In this work, we have investigated the fine structure of the lipid A isolated from Y. pseudotuberculosis lipopolysaccharide using chemical analyses, gas chromatography/mass spectrometry, plasma desorption mass spectrometry, and matrix-assisted laser desorption mass spectrometry. Arabinose (Ara) and aminoarabinose (Ara-4N) esterified the phosphates as in Yersinia pestis lipid A. The acylation of Y. pseudotuberculosis lipid A differed from those found in Yersinia enterocolitica, Yersinia ruckeri, and Y. pestis lipopolysaccharides (LPSs): in the distribution of fatty acids between the two glucosamines in the fully acylated hexaacyl molecular species and by the acyloxyacyl substitution at position C-2′, where the Y. pseudotuberculosis lipid A has a C14OC16 making it closest to that of Y. pestis.

  lipid A, endotoxin, Yersinia pseudotuberculosis, Y. pseudotuberculosis, PDMS, MALDI

  Publication DOI: 10.1016/S1387-3806(02)00706-6
  Journal NLM ID: 101137096
  Publisher: Elsevier
  Correspondence: martine.caroff@bbmpc.u-psud.fr
  Institutions: Equipe “Endotoxines”, UMR 8619 du CNRS, I.B.B.M.C., Université de Paris-Sud, F-Orsay, France, Institute for Biological Sciences, NRC, Ottawa, Ont., Canada
  Methods: GC-MS, SDS-PAGE, TLC, ESI-MS, GC, MALDI-TOF MS, composition analysis, mild alkaline degradation, PD-MS
  
   
  
  Yersinia pestis, Yersinia ruckeri
  CSDB ID 31386 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_534864

• Article ID: 1436
  Aussel L, Therisod H, Karibian D, Perry MB, Bruneteau M, Caroff M "Novel variation of lipid A structures in strains of different Yersinia species" - FEBS Letters 465(1) (2000) 87-92
  

  The Yersinia genus includes human and animal pathogens (plague, enterocolitis). The fine structures of the endotoxin lipids A of seven strains of Yersinia enterocolitica, Yersinia ruckeri and Yersinia pestis were determined and compared using mass spectrometry. These lipids differed in secondary acylation at C-2′: this was dodecanoic acid (C12) for two strains of Y. enterocolitica and Y. ruckeri, tetradecanoic acid (C14) in two other Y. enterocolitica and hexadecenoic acid (C16:1) in Y. pestis. The enterocolitica lipids having a mass identical to that of Escherichia coli were found to be structurally different. The results supported the idea of a relation between membrane fluidity and environmental adaptability in Yersinia.

  structure, lipid A, endotoxin, lipid A structure, Yersinia, Yersinia enterocolitica, plasma desorption mass spectrometry

  Publication DOI: 10.1016/S0014-5793(99)01722-6
  Journal NLM ID: 0155157
  Publisher: Elsevier
  Correspondence: martine.caro¡@bbmpc.u-psud.fr
  Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada, Equipe ‘Endotoxines’, UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Université de Paris-Sud, F-Orsay, France, Université Claude Bernard Lyon 1, UMR 5557 du Centre National de la Recherche Scientifique, F-69622 Villeurbanne, France
  Methods: GC-MS, de-O-acylation, TLC, PD-MS, UV, extraction
  
   
  
  Yersinia pestis
  CSDB ID 31641 (all data & tools)
• Article ID: 6385
  Fux AC, Casonato Melo C, Michelini S, Swartzwelter BJ, Italiani P, Himly M "Heterogeneity of Lipopolysaccharide as Source of Variability in Bioassays and LPS-Binding Proteins as Remedy" - International Journal of Molecular Sciences 24(9) (2023) 8395
  

  Lipopolysaccharide (LPS), also referred to as endotoxin, is the major component of Gram-negative bacteria's outer cell wall. It is one of the main types of pathogen-associated molecular patterns (PAMPs) that are known to elicit severe immune reactions in the event of a pathogen trespassing the epithelial barrier and reaching the bloodstream. Associated symptoms include fever and septic shock, which in severe cases, might even lead to death. Thus, the detection of LPS in medical devices and injectable pharmaceuticals is of utmost importance. However, the term LPS does not describe one single molecule but a diverse class of molecules sharing one common feature: their characteristic chemical structure. Each bacterial species has its own pool of LPS molecules varying in their chemical composition and enabling the aggregation into different supramolecular structures upon release from the bacterial cell wall. As this heterogeneity has consequences for bioassays, we aim to examine the great variability of LPS molecules and their potential to form various supramolecular structures. Furthermore, we describe current LPS quantification methods and the LPS-dependent inflammatory pathway and show how LPS heterogeneity can affect them. With the intent of overcoming these challenges and moving towards a universal approach for targeting LPS, we review current studies concerning LPS-specific binders. Finally, we give perspectives for LPS research and the use of LPS-binding molecules.

  Lipopolysaccharide, lipid A, immunology, endotoxin, detection, low endotoxin recovery, LPS-binding molecules

  NCBI PubMed ID: 37176105
  Publication DOI: 10.3390/ijms24098395
  Journal NLM ID: 101092791
  Publisher: Basel, Switzerland: MDPI
  Correspondence: M. Himly
  Institutions: Division of Allergy & Immunology, Department of Biosciences & Medical Biology, Paris Lodron University of Salzburg (PLUS), Hellbrunnerstra?e 34, 5020 Salzburg, Austria, Chemical Biology Department, R&D Reagents, Miltenyi Biotec B.V. & Co. KG, Friedrich-Ebert-Straße 68, 51429 Bergisch Gladbach, Germany, Biotechnical Faculty, Department of Biology, University of Ljubljana, Večna pot 111, 1000 Ljubljana, Slovenia, Department of Microbiology, Immunology, and Pathology, 1601 Campus Delivery, Colorado State University, Fort Collins, CO 80523, USA, Experimental Medical Physics, Heinrich-Heine University Düsseldorf, Universitätsstraße 1, 40225 Düsseldorf, Germany, Institute of Biochemistry and Cell Biology, Consiglio Nazionale delle Ricerche (CNR), Via P. Castellino 111, 80131 Naples, Italy, Stazione Zoologica Anton Dohrn (SZN), Villa Comunale, 80121 Naples, Italy
  
   
  
  Yersinia pestis Yreka
  CSDB ID 10691 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_534864

• Article ID: 1449
  Kawahara K, Tsukano H, Watanabe H, Lindner B, Matsuura M "Modification of the structure and activity of lipid A in Yersinia pestis lipopolysaccharide by growth temperature" - Infection and Immunity 70(8) (2002) 4092-4098
  

  Yersinia pestis strain Yreka was grown at 27 or 37°C, and the lipid A structures (lipid A-27°C and lipid A-37°C) of the respective lipopolysaccharides (LPS) were investigated by matrix-assisted laser desorption ionization-time-of-flight (MALDI-TOF) mass spectrometry. Lipid A-27°C consisted of a mixture of tri-acyl, tetra-acyl, penta-acyl, and hexa-acyl lipid A's, of which tetra-acyl lipid A was most abundant. Lipid A-37°C consisted predominantly of tri- and tetra-acylated molecules, with only small amounts of penta-acyl lipid A; no hexa-acyl lipid A was detected. Furthermore, the amount of 4-amino-arabinose was substantially higher in lipid A-27°C than in lipid A-37°C. By use of mouse and human macrophage cell lines, the biological activities of the LPS and lipid A preparations were measured via their abilities to induce production of tumor necrosis factor alpha (TNF-α). In both cell lines the LPS and the lipid A from bacteria grown at 27°C were stronger inducers of TNF-α than those from bacteria grown at 37°C. However, the difference in activity was more prominent in human macrophage cells. These results suggest that in order to reduce the activation of human macrophages, it may be more advantageous for Y. pestis to produce less-acylated lipid A at 37°C.

  Lipopolysaccharide, structure, lipid, lipid A, activity, modification, Yersinia, Yersinia pestis, growth, temperature, cation

  Publication DOI: 10.1128/IAI.70.8.4092-4098.2002
  Journal NLM ID: 0246127
  Publisher: American Society for Microbiology
  Correspondence: kawahara-k@kitasato.or.jp
  Institutions: Department of Bacteriology, The Kitasato Institute, Tokyo 108-8642, Department of Bacteriology, National Institute of Infectious Diseases, Tokyo 162-8640, Department of Microbiology, Jichi Medical School, Tochigi 329-0498, Japan, Division of Biophysics, Research Center Borstel, D-23845 Borstel, Germany
  Methods: SDS-PAGE, sugar analysis, GLC, chemical methods, MALDI-TOF MS, extraction, TNF-α assay
  
   
  
  Yersinia pestis Yreka
  CSDB ID 868 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: lipid A
Contained glycoepitopes: IEDB_135394,IEDB_135515,IEDB_141807,IEDB_151531,IEDB_534864

• Article ID: 1461
  Holst O "Lipopolysaccharides of Yersinia. An overview" - Book: The Genus Yersinia (series: Advances in Experimental Medicine and Biology) (2003) Vol. 529, 219-228
  

  no abstract

  Lipopolysaccharide, lipopolysaccharides, structure, core, strain, cell, cell wall, PAGE, function, genus, bacteriophage, Yersinia, Yersinia pestis, influence, Bacteriophages, growth, temperature

  NCBI PubMed ID: 12756761
  Publication DOI: 10.1007/0-306-48416-1_43
  Publisher: Kluwer Academic/Plenum Publishers, New York
  Correspondence: oholst@fz-borstel.de
  Editors: Skurnik M, Bengoechea JA, Granfors K
  Institutions: Division of Structural Biochemistry, Research Center Borstel, Borstel, Germany
  
   
  
  Yersinia pestis Yreka
  CSDB ID 31822 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: lipid A
Contained glycoepitopes: IEDB_141807,IEDB_151531,IEDB_534864

• Article ID: 6089
  Knirel YA, Anisimov AP, Kislichkina AA, Kondakova AN, Bystrova OV, Vagaiskaya AS, Shatalin KY, Shashkov AS, Dentovskaya SV "Lipopolysaccharide of the Yersinia pseudotuberculosis Complex" - Biomolecules 11(10) (2021) 1410
  

  Lipopolysaccharide (LPS), localized in the outer leaflet of the outer membrane, serves as the major surface component of the Gram-negative bacterial cell envelope responsible for the activation of the host's innate immune system. Variations of the LPS structure utilized by Gram-negative bacteria promote survival by providing resistance to components of the innate immune system and preventing recognition by TLR4. This review summarizes studies of the biosynthesis of Yersinia pseudotuberculosis complex LPSs, and the roles of their structural components in molecular mechanisms of yersiniae pathogenesis and immunogenesis.

  core, Pathogenesis, lipid A, Yersinia pseudotuberculosis, lipopolysaccharide (LPS), Yersinia pestis, Plague, pathogenicity factor

  NCBI PubMed ID: 34680043
  Publication DOI: 10.3390/biom11101410
  Journal NLM ID: 101596414
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Y.A. Knirel ; S.V. Dentovskaya
  Institutions: Laboratory of Carbohydrate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Laboratory for Plague Microbiology, Especially Dangerous Infections Department, State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA
  
   
  
  Yersinia pestis, Yersinia pseudotuberculosis
  CSDB ID 9653 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: lipid A
Contained glycoepitopes: IEDB_141807,IEDB_151531

• Article ID: 6089
  Knirel YA, Anisimov AP, Kislichkina AA, Kondakova AN, Bystrova OV, Vagaiskaya AS, Shatalin KY, Shashkov AS, Dentovskaya SV "Lipopolysaccharide of the Yersinia pseudotuberculosis Complex" - Biomolecules 11(10) (2021) 1410
  

  Lipopolysaccharide (LPS), localized in the outer leaflet of the outer membrane, serves as the major surface component of the Gram-negative bacterial cell envelope responsible for the activation of the host's innate immune system. Variations of the LPS structure utilized by Gram-negative bacteria promote survival by providing resistance to components of the innate immune system and preventing recognition by TLR4. This review summarizes studies of the biosynthesis of Yersinia pseudotuberculosis complex LPSs, and the roles of their structural components in molecular mechanisms of yersiniae pathogenesis and immunogenesis.

  core, Pathogenesis, lipid A, Yersinia pseudotuberculosis, lipopolysaccharide (LPS), Yersinia pestis, Plague, pathogenicity factor

  NCBI PubMed ID: 34680043
  Publication DOI: 10.3390/biom11101410
  Journal NLM ID: 101596414
  Publisher: Basel, Switzerland: MDPI
  Correspondence: Y.A. Knirel ; S.V. Dentovskaya
  Institutions: Laboratory of Carbohydrate Chemistry, N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Laboratory for Plague Microbiology, Especially Dangerous Infections Department, State Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY 10016, USA
  
   
  
  Yersinia pestis, Yersinia pseudotuberculosis
  CSDB ID 9656 (all data & tools)