Found 19 structures.
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1. Compound ID: 374
Structure type: oligomer
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_142345
The structure is contained in the following publication(s):
- Article ID: 118
Toth A, Medgyes A, Bajza I, Lipták A, Batta G, Kontrohr T, Peterffy K, Pozsgay V "Synthesis of the repeating unit of the O-specific polysaccharide of Shigella sonnei and quantitation of its serologic activity" -
Bioorganic and Medicinal Chemistry Letters 10(1) (2000) 19-21
The chemical synthesis of the zwitterionic disaccharide 2 is described that corresponds to the repeating unit of the O-specific polysaccharide (1) of the gram-negative human pathogen Shigella sonnei. Passive hemolysis inhibition tests using a hyperimmune rabbit serum raised against S. sonnei showed that the serologic activity of the disaccharide 2 is nearly 2- to 3-fold higher than those of its component monosaccharides. NMR data of 2 are in support of the proposed structure of the O-specific polysaccharide.
synthesis, polysaccharide, repeating unit, O-specific, O-specific polysaccharide, activity, quantitation, serologic, Shigella, Shigella sonnei
NCBI PubMed ID: 10636234Journal NLM ID: 9107377Publisher: Elsevier
Correspondence: liptaka@tigris.klte.hu
Institutions: Research Group for Carbohydrates of the Hungarian Academy of Sciences and Institute of Biochemistry, Lajos Kossuth University, Debrecen, Hungary, H-4010, Research Group for Antibiotics of the Hungarian Academy of Sciences, Debrecen, Hungary, Institute of Microbiology, University Medical School, Pecs, Hungary, Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892, USA.
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2. Compound ID: 558
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_142345
The structure is contained in the following publication(s):
- Article ID: 118
Toth A, Medgyes A, Bajza I, Lipták A, Batta G, Kontrohr T, Peterffy K, Pozsgay V "Synthesis of the repeating unit of the O-specific polysaccharide of Shigella sonnei and quantitation of its serologic activity" -
Bioorganic and Medicinal Chemistry Letters 10(1) (2000) 19-21
The chemical synthesis of the zwitterionic disaccharide 2 is described that corresponds to the repeating unit of the O-specific polysaccharide (1) of the gram-negative human pathogen Shigella sonnei. Passive hemolysis inhibition tests using a hyperimmune rabbit serum raised against S. sonnei showed that the serologic activity of the disaccharide 2 is nearly 2- to 3-fold higher than those of its component monosaccharides. NMR data of 2 are in support of the proposed structure of the O-specific polysaccharide.
synthesis, polysaccharide, repeating unit, O-specific, O-specific polysaccharide, activity, quantitation, serologic, Shigella, Shigella sonnei
NCBI PubMed ID: 10636234Journal NLM ID: 9107377Publisher: Elsevier
Correspondence: liptaka@tigris.klte.hu
Institutions: Research Group for Carbohydrates of the Hungarian Academy of Sciences and Institute of Biochemistry, Lajos Kossuth University, Debrecen, Hungary, H-4010, Research Group for Antibiotics of the Hungarian Academy of Sciences, Debrecen, Hungary, Institute of Microbiology, University Medical School, Pecs, Hungary, Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 20892, USA.
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3. Compound ID: 832
Structure type: polymer biological repeating unit
Aglycon: PP-undecaprenol
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_142345
The structure is contained in the following publication(s):
- Article ID: 229
Xu DQ, Cisar JO, Ambulos JN, Burr DH, Kopecko DJ "Molecular cloning and characterization of genes for Shigella sonnei form I O polysaccharide: proposed biosynthetic pathway and stable expression in a live salmonella vaccine vector" -
Infection and Immunity 70(8) (2002) 4414-4423
The gene region for biosynthesis of Shigella sonnei form I O polysaccharide (O-Ps) and flanking sequences, totaling >18 kb, was characterized by deletion analysis to define a minimal construct for development of Salmonella-based live vaccine vector strains. Lipopolysaccharide (LPS) expression and DNA sequence studies of plasmid deletion derivatives indicated form I O-Ps expression from a 12.3-kb region containing a putative promoter and 10 contiguous open reading frames (ORFs), one of which is the transposase of IS630. A detailed biosynthetic pathway, consistent with the predicted functions of eight of the nine essential ORFs and the form I O-Ps structure, is proposed. Further sequencing identified partial IS elements (i.e., IS91 and IS630) and wzz upstream of the form I coding region and a fragment of aqpZ and additional full or partial IS elements (i.e., IS629, IS91, and IS911) downstream of this region. The stability of plasmid-based form I O-Ps expression was greater from low-copy vectors than from high-copy vectors and was enhanced by deletion of the downstream IS91 from plasmid inserts. Both core-linked (i.e., LPS) and non-core-linked (i.e., capsule-like) surface expression of form I O-Ps were detected by Western blotting and silver staining of polyacrylamide gel electrophoresisseparated Shigella and Escherichia coli extracts. However, salmonellae, which have a core that is chemically dissimilar to that of shigellae, expressed only non-core-linked surface-associated form I O-Ps. Finally, attenuated Salmonella enterica serovar Typhi live vaccine vector candidates, containing minimal-sized form I operon constructs, elicited immune protection in mice against virulent S. sonnei challenge, thereby supporting the promise of live, oral vaccines for the prevention of shigellosis.
biosynthesis, biosynthetic, expression, gene, Bacterial, genetics, microbiology, Sequence Analysis, DNA, characterization, polysaccharide, molecular, form, cloning, animal, Carbohydrate Sequence, immunology, mice, Molecular Sequence Data, O-polysaccharide, Genes, O antigens, O polysaccharide, Salmonella, region, vaccines, Shigella, Shigella sonnei, pathway, Base Sequence, Disease Models, Dysentery, Bacillary, gene expression, Genetic Vectors, Inbred ICR, prevention & control, Salmonella typhi, Shigella Vaccines, vaccine, Attenuated, Synthetic
NCBI PubMed ID: 12117952Publication DOI: 10.1128/IAI.70.8.4414-4423.2002Journal NLM ID: 0246127Publisher: American Society for Microbiology
Correspondence: Kopecko@cber.fda.gov
Institutions: Oral Infection and Immunity Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Laboratory of Enteric and Sexually Transmitted Diseases, Center for Biologics Evaluation and Research, Food, Drug Administration,4 Bethesda, MD, USA, Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland 212012, Division of Virulence Assessment, Center for Food Safety and Nutrition, Food and Drug Administration, Laurel, Maryland 207083
Methods: genetic methods
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4. Compound ID: 2809
Structure type: monomer
The structure is contained in the following publication(s):
- Article ID: 984
Medgyes A, Farkas E, Lipták A, Pozsgay V "Synthesis of the monosaccharide units of the O-specific polysaccharide of Shigella sonnei" -
Tetrahedron 53(12) (1997) 4159-4178
The monosaccharide components of the O-specific polysaccharide 1 of the lipopolysaccharide of the enteropathogenic bacterium Shigella sonnei were synthesized as their methyl glycosides 2 and 3 in their natural anomeric form. The key intermediate to the diaminotrideoxygalactose derivative 2 was ethyl 3-O-acetyl-2-deoxy-2-phthalimido-1-thio-β-D-glucopyranoside (9) that was converted to its ditosylate 10. Regioselective deoxygenation at C-6 followed by nucleophilic displacement of the secondary tosyloxy group by azide afforded the 4-azido thioglycoside 13. Methyl trifluoromethanesulfonate-assisted methanolysis of 13 gave the O-glycoside 14. Replacement of the phthalimido by an acetamido group followed by catalytic reduction of the azido group led to the diamino-trideoxygalactose derivative 2. The precursor to the l-altruronic acid derivative 3 was methyl α-L-glucopyranoside (19) that was routinely converted to the benzylidene-protected 2,3-anhydro-allopyranoside 22. Regioselective opening of the epoxide ring by NaN3 afforded the 2-azido derivative 23 that was benzylated at HO-3. Hydrolytic removal of the benzylidene group followed by TEMPO oxidation of C-6 and subsequent esterification with MeI gave the key l-azido-altruronic acid intermediate 29 that was transformed to the acetamidoaltruronic acid derivative 3. High resolution NMR data of the altruronic acid derivatives indicate that the conformation of their pyranose ring is crucially dependent on the substitution pattern: the 2-azido altruronic acid derivatives prefer the 4C1 conformation whereas the 2-acetamido congeners exist preferentially in the 1C4 conformation.
Lipopolysaccharide, synthesis, LPS, polysaccharide, O-specific, O-specific polysaccharide, polysaccharides, Shigella, Shigella sonnei, 4, galactose, monosaccharide, 2-acetamido-2-deoxy-L-altruronic acid, 2-acetamido-4-amino-2, 6-trideoxy-D-, carbohydrate antigen, glycoconjugate vaccine, monosaccharides, TEMPO oxidation
Publication DOI: 10.1016/S0040-4020(97)00145-2Journal NLM ID: 2984170RPublisher: Pergamon Press
Institutions: Research Group for Carbohydrates of the Hungarian Academy of Sciences P.O.B. 55, H-4010 Debrecen, Hungary, Institute of Biochemistry, Kossuth Lajos University, H-4010 Debrecen, Hungary, Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2720, U.S.A.
Methods: 13C NMR, 1H NMR, TLC, CIMS MS, elemental analysis
- Article ID: 1368
Batta G, Lipták A, Schneerson R, Pozsgay V "Conformational stabilization of the altruronic acid residue in the O-specific polysaccharide of Shigella sonnei/Plesiomonas shigelloides" -
Carbohydrate Research 305(1) (1997) 93-99
Complete assignments for the 1H- and the 13C-NMR spectra of the O-specific polysaccharide of S. sonnei/Plesiomonas shigelloides are reported. Evidence is presented that in this polysaccharide both pyranose residues exist preferentially in the 4C1 chair conformation and that the polysaccharide exists in the zwitterion form.
NMR spectroscopy, O-specific polysaccharide, Shigella sonnei, Plesiomonas shigelloides, altruronic acid, polysaccharide conformation
NCBI PubMed ID: 9534229Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: vipo@helix.nih.gov
Institutions: Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health,Bethesda
Methods: 13C NMR, 1H NMR, CIMS MS, ROESY, TOCSY
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5. Compound ID: 3651
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_142345
The structure is contained in the following publication(s):
- Article ID: 1368
Batta G, Lipták A, Schneerson R, Pozsgay V "Conformational stabilization of the altruronic acid residue in the O-specific polysaccharide of Shigella sonnei/Plesiomonas shigelloides" -
Carbohydrate Research 305(1) (1997) 93-99
Complete assignments for the 1H- and the 13C-NMR spectra of the O-specific polysaccharide of S. sonnei/Plesiomonas shigelloides are reported. Evidence is presented that in this polysaccharide both pyranose residues exist preferentially in the 4C1 chair conformation and that the polysaccharide exists in the zwitterion form.
NMR spectroscopy, O-specific polysaccharide, Shigella sonnei, Plesiomonas shigelloides, altruronic acid, polysaccharide conformation
NCBI PubMed ID: 9534229Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: vipo@helix.nih.gov
Institutions: Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health,Bethesda
Methods: 13C NMR, 1H NMR, CIMS MS, ROESY, TOCSY
- Article ID: 1778
Knirel YA, Kochetkov NK "The structure of lipopolysaccharides of gram-negative bacteria. III. The structure of O-antigens: A review" -
Biochemistry (Moscow) 59(12) (1994) 1325-1383
This review summarizes data on the composition and structure of the O-antigens, the polysaccharide chains of the outer-membrane lipopolysaccharides (LPS) of Gram-negative bacteria defining the immunospecificity of these microbial cells. Special reference is given to some structural features of the O-antigens, such as the presence of unique monosaccharides and noncarbohydrate components, masked regularity, and the occurrence in one microorganism of LPS with structurally different polysaccharide chains. Antigenic relationships between microorganisms belonging to different taxonomic groups are discussed.
structure, O-antigen, chemical composition, bacterial lipopolysaccharides, Salmonella livingstone C1
NCBI PubMed ID: 7533007Journal NLM ID: 0376536Publisher: Nauka/Interperiodica
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 1976
Kenne L, Lindberg B, Petersson K, Katzenellenbogen E, Romanowska E "Structural studies of the O-specific side-chains of the Shigella sonnei phase I lipopolysaccharide" -
Carbohydrate Research 78(1) (1980) 119-126
The structure of the O-specific side-chains of the Shigella sonnei phase I lipopolysaccharide has been investigated. The side chains are composed of disaccharide repeating-units containing two uncommon sugar components, one of witch, 2-amino-2-deoxy-L-altruronic acid, has been identified previously. The other has now been identified as 2-acetamido-4-amino-2,4,6-trideoxy-D-galactose. The uronic acid, as N-acetylated α-pyranosyl residues, is linked through O-4, and the diamino sugar, as β-pyranosyl residues, is linked through O-3. The pyranosyluronic acid residue assumes the 4C1 conformation in the polymer, with the carboxyl group in the axial position.
Publication DOI: 10.1016/S0008-6215(00)83665-4Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Stockholm, Sweden, udwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw Poland
Methods: 13C NMR
- Article ID: 2832
Seltmann G, Knirel YA, Shashkov AS, Tschape H "Characterization of a lipopolysaccharide encoded by a recombinant Shigella sonnei plasmid in Escherichia coli K-12" -
Zentralblatt für Bakteriologie - International Journal of Medical Microbiology 277 (1992) 419-428
- Article ID: 3506
Liu B, Knirel YA, Feng L, Perepelov AV, Senchenkova SN, Wang Q, Reeves P, Wang L "Structure and genetics of Shigella O antigens" -
FEMS Microbiology Reviews 32(4) (2008) 627-653
This review covers the O antigens of the 46 serotypes of Shigella, but those of most Shigella flexneri are variants of one basic structure, leaving 34 Shigella distinct O antigens to review, together with their gene clusters. Several of the structures and gene clusters are reported for the first time and this is the first such group for which structures and DNA sequences have been determined for all O antigens. Shigella strains are in effect Escherichia coli with a specific mode of pathogenicity, and 18 of the 34 O antigens are also found in traditional E. coli. Three are very similar to E. coli O antigens and 13 are unique to Shigella strains. The O antigen of Shigella sonnei is quite atypical for E. coli and is thought to have transferred from Plesiomonas. The other 12 O antigens unique to Shigella strains have structures that are typical of E. coli, but there are considerably more anomalies in their gene clusters, probably reflecting recent modification of the structures. Having the complete set of structures and genes opens the way for experimental studies on the role of this diversity in pathogenicity.
structure, O antigen, Shigella, O antigen gene cluster, O antigen diversity
NCBI PubMed ID: 18422615Publication DOI: 10.1111/j.1574-6976.2008.00114.xJournal NLM ID: 8902526Publisher: Oxford University Press
Correspondence: wanglei@nankai.edu.cn
Institutions: TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China, TEDA School of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin, China.
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, ESI-MS, serological methods, genetic methods, biochemical methods
- Article ID: 4329
Knirel YA "Structure of O-antigens" -
Book: Bacterial lipopolysaccharides: Structure, chemical synthesis, biogenesis and interaction with host cells (2011) Chapter 3, 41-115
The lipopolysaccharide (LPS) is the major constituent of the outer leaflet of the outer membrane of Gram-negative bacteria. Its lipid A moiety is embedded in the membrane and serves as an anchor for the rest of the LPS molecule. The outermost repetitive glycan region of the LPS is linked to the lipid A through a core oligosaccharide (OS), and is designated as the O-specific polysaccharide (O-polysaccharide, OPS) or O-antigen. The O-antigen is the most variable portion of the LPS and provides serological specificity, which is used for bacterial serotyping. The OPS also provides protection to the microorganisms from host defenses such as complement mediated killing and phagocytosis, and is involved in interactions of bacteria with plants and bacteriophages. Studies of the OPSs ranging from the elucidation of their chemical structures and conformations to their biological and physico-chemical properties help improving classification schemes of Gram-negative bacteria. Furthermore, these studies contributed to a better understanding of the mechanisms of pathogenesis of infectious diseases, as well as provided information to develop novel vaccines and diagnostic reagents.
Lipopolysaccharide, synthesis, lipopolysaccharides, structure, Bacterial, host, O-antigen, O antigen, cell, O antigens, O-antigens, chemical, interaction, cells, PDF, chemical synthesis, biogenesis
Publication DOI: 10.1007/978-3-7091-0733-1_3Publisher: Springer
Correspondence: knirel@ioc.ac.ru
Editors: Knirel YA, Valvano MA
Institutions: Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Article ID: 4524
Gozdziewicz TK, Lugowski C, Lukasiewicz J "First evidence for a covalent linkage between enterobacterial common antigen and lipopolysaccharide in Shigella sonnei phase II ECALPS" -
Journal of Biological Chemistry 289(5) (2014) 2745-2754
Enterobacterial common antigen (ECA) is expressed by Gram-negative bacteria belonging to Enterobacteriaceae, including emerging drug-resistant pathogens such as Escherichia coli, Klebsiella pneumoniae, and Proteus spp. Recent studies have indicated the importance of ECA for cell envelope integrity, flagellum expression, and resistance of enteric bacteria to acetic acid and bile salts. ECA, a heteropolysaccharide built from the trisaccharide repeating unit, →3)-α-D-Fucp4NAc-(1→4)-β-D-ManpNAcA-(1→4)-α-D-GlcpNAc-(1→, occurs as a cyclic form (ECACYC), a phosphatidylglycerol (PG)-linked form (ECAPG), and an endotoxin/lipopolysaccharide (LPS)-associated form (ECALPS). Since the discovery of ECA in 1962, the structures of ECAPG and ECACYC have been completely elucidated. However, no direct evidence has been presented to support a covalent linkage between ECA and LPS; only serological indications of co-association have been reported. This is paradoxical, given that ECA was first identified based on the capacity of immunogenic ECALPS to elicit antibodies cross-reactive with enterobacteria. Using a simple isolation protocol supported by serological tracking of ECA epitopes and NMR spectroscopy and mass spectrometry, we have succeeded in the first detection, isolation, and complete structural analysis of poly- and oligosaccharides of Shigella sonnei phase II ECALPS. ECALPS consists of the core oligosaccharide substituted with one to four repeating units of ECA at the position occupied by the O-antigen in the case of smooth S. sonnei phase I. These data represent the first structural evidence for the existence of ECALPS in the half-century since it was first discovered and provide insights that could prove helpful in further structural analyses and screening of ECALPS among Enterobacteriaceae species.
Escherichia coli, enterobacterial common antigen, ECA, Shigella sonnei, Klebsiella pneumoniae, cell envelope, flagella, mass spectrometry (MS), hase II
NCBI PubMed ID: 24324266Publication DOI: 10.1074/jbc.M113.512749Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: czaja@iitd.pan.wroc.pl
Institutions: Department of Immunochemistry, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, SDS-PAGE, 31P NMR, ESI-MS, mild acid hydrolysis, composition analysis, NMR-1D, serological methods, immunoblotting
- Article ID: 4570
Maciejewska A, Lukasiewicz J, Kaszowska M, Man-Kupisinska A, Jachymek W, Lugowski C "Core oligosaccharide of Plesiomonas shigelloides PCM 2231 (Serotype O17) lipopolysaccharide--structural and serological analysis" -
Marine Drugs 11(2) (2013) 440-454
The herein presented complete structure of the core oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 2231 (serotype O17) was investigated by (1)H, (13)C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The core oligosaccharide is composed of an undecasaccharide, which represents the second core type identified for P. shigelloides serotype O17 LPS. This structure is similar to that of the core oligosaccharide of P. shigelloides strains 302-73 (serotype O1) and 7-63 (serotype O17) and differs from these only by one sugar residue. Serological screening of 55 strains of P. shigelloides with the use of serum against identified core oligosaccharide conjugated with bovine serum albumin (BSA) indicated the presence of similar structures in the LPS core region of 28 O-serotypes. This observation suggests that the core oligosaccharide structure present in strain PCM 2231 could be the most common type among P. shigelloides lipopolysaccharides.
Lipopolysaccharide, core oligosaccharide, endotoxin, Plesiomonas shigelloides
NCBI PubMed ID: 23389090Publication DOI: 10.3390/md11020440Journal NLM ID: 101213729Publisher: Basel, Switzerland: Molecular Diversity Preservation International
Correspondence: aniaaugustyniuk@iitd.pan.wroc.pl
Institutions: Department of Immunochemistry, Ludwik Hirszfeld Institute Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Wroclaw, Poland
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, SDS-PAGE, sugar analysis, mild acid hydrolysis, MALDI-TOF MS, NMR-1D, serological methods, immunoblotting, mild acid hydrolysismethylation
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6. Compound ID: 3652
Structure type: monomer
The structure is contained in the following publication(s):
- Article ID: 1368
Batta G, Lipták A, Schneerson R, Pozsgay V "Conformational stabilization of the altruronic acid residue in the O-specific polysaccharide of Shigella sonnei/Plesiomonas shigelloides" -
Carbohydrate Research 305(1) (1997) 93-99
Complete assignments for the 1H- and the 13C-NMR spectra of the O-specific polysaccharide of S. sonnei/Plesiomonas shigelloides are reported. Evidence is presented that in this polysaccharide both pyranose residues exist preferentially in the 4C1 chair conformation and that the polysaccharide exists in the zwitterion form.
NMR spectroscopy, O-specific polysaccharide, Shigella sonnei, Plesiomonas shigelloides, altruronic acid, polysaccharide conformation
NCBI PubMed ID: 9534229Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: vipo@helix.nih.gov
Institutions: Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health,Bethesda
Methods: 13C NMR, 1H NMR, CIMS MS, ROESY, TOCSY
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7. Compound ID: 5551
|
a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-2)-a-D-Galp-(1-2)-+ | EtN-(1---P---P---4)-+
| | |
a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-3)-b-D-Glcp-(1-3)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdo |
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Structure type: oligomer
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130670,IEDB_131186,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 2369
Gamian A, Romanowska E "The core structure of Shigella sonnei lipopolysaccharide and the linkage between O-specific polysaccharide and the core region" -
European Journal of Biochemistry 129 (1982) 105-109
No abstract available
NCBI PubMed ID: 6761115Publication DOI: 10.1111/j.1432-1033.1982.tb07027.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Zaklad Immunochemii, Instytut Immunologii i Terapii Doswiadezalnej imienia Ludwika Hirszfelda, Polska Akademia Nauk, ul. Czerska 12, PL‐53‐114 Wroclaw, Poland
Methods: 1H NMR
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8. Compound ID: 5553
|
a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-7)-+
|
a-D-Galp-(1-2)-a-D-Galp-(1-2)-+ | EtN-(1---P---P---4)-+
| | |
a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-3)-b-D-Glcp-(1-3)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdo |
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Structure type: oligomer
Compound class: LPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130670,IEDB_131186,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 2369
Gamian A, Romanowska E "The core structure of Shigella sonnei lipopolysaccharide and the linkage between O-specific polysaccharide and the core region" -
European Journal of Biochemistry 129 (1982) 105-109
No abstract available
NCBI PubMed ID: 6761115Publication DOI: 10.1111/j.1432-1033.1982.tb07027.xJournal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Zaklad Immunochemii, Instytut Immunologii i Terapii Doswiadezalnej imienia Ludwika Hirszfelda, Polska Akademia Nauk, ul. Czerska 12, PL‐53‐114 Wroclaw, Poland
Methods: 1H NMR
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9. Compound ID: 5804
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide, O-antigen
The structure is contained in the following publication(s):
- Article ID: 2548
Taylor DN, Trofa AC, Sadoff J, Chu C, Bryla D, Shiloach J, Cohen D, Ashkenazi S, Lerman Y, Egan W, Schneerson R, Robbins JB "Synthesis, characterization, and clinical evaluation of conjugate vaccines composed of the O-specific polysaccharides of Shigella dysenteriae type 1, Shigella flexneri type 2a, and Shigella sonnei (Plesiomonas shigelloides) bound to bacterial toxoids" -
Infection and Immunity 61 (1993) 3678-3687
The theoretic basis for developing conjugate vaccines, to induce immunoglobulin G (IgG) lipopolysaccharide (LPS) antibodies for the prevention of shigellosis, has been described (J. B. Robbins, C.-Y. Chu, and R. Schneerson, Clin. Infect. Dis. 15:346-361, 1992). The O-specific polysaccharides (O-SPs) of Shigella dysenteriae type 1, S. flexneri type 2a, and S. sonnei were covalently bound to carrier proteins. Alone, the O-SPs were not immunogenic in mice. Conjugates of these O-SPs, injected into young outbred mice subcutaneously as saline solutions containing 2.5 micrograms of saccharide, elicited serum IgG and IgM antibodies with booster responses; adsorption onto alum enhanced their immunogenicity. Injection of 25 micrograms of these conjugates into adult volunteers elicited mild local reactions only. Each conjugate induced a significant rise of the geometric mean serum IgG, IgM, and IgA LPS antibody levels. A second injection 6 weeks later did not elicit booster responses, and adsorption of the conjugates onto alum did not enhance their immunogenicity. Conjugate-induced levels of IgA, but not IgG or IgM, declined to preimmunization levels at day 56. The levels of postimmunization antibodies of the three immunoglobulin classes were similar to or higher than those of recruits in the Israel Defense Force following shigellosis caused by S. flexneri type 2a or S. sonnei. These data provide the basis for evaluating these conjugates to prevent shigellosis.
NCBI PubMed ID: 8359890Journal NLM ID: 0246127Publisher: American Society for Microbiology
Institutions: Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20307-5100
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10. Compound ID: 7880
|
a-D-GalpN-(1-4)-a-D-GalpA-(1-7)-L-gro-a-D-manHepp-(1-7)-+ b-D-Galp-(1-4)-+
| |
a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-b-D-GlcpNAc-(1-6)-a-D-GlcpN-(1-4)-a-D-GalpA-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdo
|
50%b-D-Glcp-(1-2)-+ |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130670,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 3497
Kubler-Kielb J, Schneerson R, Mocca C, Vinogradov E "The elucidation of the structure of the core part of the LPS from Plesiomonas shigelloides serotype O17 expressing O-polysaccharide chain identical to the Shigella sonnei O-chain" -
Carbohydrate Research 343(18) (2008) 3123-3127
Plesiomonas shigelloides O17 LPS contains the same O-antigenic polysaccharide chain as a causative agent of dysentery, Shigella sonnei. This polysaccharide can be used as a component of a vaccine against dysentery. Core part of the P. shigelloides O17 LPS was studied using NMR and mass spectrometry and the following structure was proposed: Significant similarity of the P. shigelloides O17 LPS core with the structure of the P. shigelloides O54 core was observed
NMR, LPS, structure, core, Shigella sonnei, O-chain, Plesiomonas, Plesiomonas shigelloides, conjugare vaccine
NCBI PubMed ID: 18954864Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: evguenii.vinogradov@nrc.ca (E. Vinogradov)
Institutions: National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, sugar analysis, GLC, mild acid hydrolysis, alkaline degradation, NMR-1D
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11. Compound ID: 8671
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?%a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-7)-+
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a-D-Galp-(1-2)-a-D-Galp-(1-2)-+ | ?%EtN-(1-0)-?%P-4)-+
| | |
a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-3)-b-D-Glcp-(1-3)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdo
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P-4)-+ |
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Structure type: oligomer
Compound class: core oligosaccharide with two O-units
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_130650,IEDB_130670,IEDB_131186,IEDB_133751,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 3770
Robbins JB, Kubler-Kielb J, Vinogradov E, Mocca C, Pozsgay V, Shiloach J, Schneerson R "Synthesis, characterization, and immunogenicity in mice of Shigella sonnei O-specific oligosaccharide-core-protein conjugates" -
Proceedings of the National Academy of Sciences of the USA 106(19) (2009) 7974-7978
Shigellosis, an enteric disease, is on the World Health Organization's priority prevention list. In one study, the Shigella sonnei O-specific polysaccharide (O-SP)-protein conjugate showed 72% protection against disease in Israeli army recruits exposed to high rates (8-14%) of infection. The protection was related to vaccine-induced IgG anti-O-SP levels. Synthetic oligosaccharides of Shigella dysenteriae type 1, bound by their reducing ends to a carrier protein ('sun'-type configuration), induced significantly higher antibody levels than the native O-SP bound to protein by multiple-point attachments ('lattice'-type configuration). Attempts to synthesize the S. sonnei O-SP based oligosaccharides were not successful. Here, we describe the isolation, characterization, and conjugation of low-molecular-mass O-SP-core (O-SPC) fragments. The O-SPC fragments were bound by their reducing ends similar to the preparation of the synthetic S. dysenteriae type 1 conjugates. The O-SPC conjugates used oxime linkages between the terminal Kdo residues at the reducing ends of the S. sonnei saccharides and aminooxy linkers bound to BSA or a recombinant diphtheria toxin. The coupling reaction was carried out at a neutral pH and room temperature. IgG antibody levels induced in young outbred mice by the S. sonnei O-SPC conjugates were significantly higher then those elicited by the O-SP conjugates. Accordingly, we propose to evaluate clinically these conjugates.
Lipopolysaccharide, Kdo, IgG, vaccine, glycoconjugate
NCBI PubMed ID: 19346477Journal NLM ID: 7505876Publisher: National Academy of Sciences
Correspondence: robbinsjo@mail.nih.gov
Institutions: National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA
Methods: 13C NMR, 1H NMR, SDS-PAGE, ELISA, MALDI-TOF MS, serological methods, statistical analysis
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12. Compound ID: 9054
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50%a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-7)-+
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a-D-Galp-(1-2)-a-D-Galp-(1-2)-+ | P-4)-+
| | |
a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-3)-b-D-Glcp-(1-3)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdo
|
P-4)-+ |
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Structure type: oligomer
Trivial name: core type R4
Compound class: core oligosaccharide with O-unit
Contained glycoepitopes: IEDB_130650,IEDB_130670,IEDB_131186,IEDB_133751,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 3883
Kubler-Kielb J, Vinogradov E, Mocca C, Pozsgay V, Coxon B, Robbins JB, Schneerson R "Immunochemical studies of Shigella flexneri 2a and 6, and Shigella dysenteriae type 1 O-specific polysaccharide-core fragments and theirprotein conjugates as vaccine candidates" -
Carbohydrate Research 345(11) (2010) 1600-1608
There is no licensed vaccine for the prevention of shigellosis. Our approach to the development of a Shigella vaccines is based on inducing serum IgG antibodies to the O-specific polysaccharide (O-SP) domain of their lipopolysaccharides (LPS). We have shown that low molecular mass O-SP-core (O-SPC) fragments isolated from Shigella sonnei LPS conjugated to proteins induced significantly higher antibody levels in mice than the full length O-SP conjugates. This finding is now extended to the O-SPC of Shigella flexneri 2a and 6, and Shigella dysenteriae type 1. The structures of O-SPC, containing core plus 1-4 O-SP repeat units (RUs), were analyzed by NMR and mass spectroscopy. The first RUs attached to the cores of S. flexneri 2a and 6 LPS were different from the following RUs in their O-acetylation and/or glucosylation. Conjugates of core plus more than 1 RU were necessary to induce LPS antibodies in mice. The resulting antibody levels were comparable to those induced by the full length O-SP conjugates. In S. dysenteriae type 1, the first RU was identical to the following RUs, with the exception that the GlcNAc was bound to the core in the β-configuration, while in all other RUs the GlcNAc was present in the α-configuration. In spite of this difference, conjugates of S. dysenteriae type 1 core with 1, 2, or 3 RUs induced LPS antibodies in mice with levels statistically higher than those of the full size O-SP conjugates. O-SPC conjugates are easy to prepare, characterize, and standardize, and their clinical evaluation is planned.
LPS, Shigella, vaccine, conjugate
NCBI PubMed ID: 20542498Publication DOI: 10.1016/j.carres.2010.05.006Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: kielbj@mail.nih.gov
Institutions: The Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD, USA
Methods: 13C NMR, 1H NMR, methylation, SDS-PAGE, sugar analysis, MALDI-TOF MS, NMR-1D, serological methods, statistical analysis, immunization, conjugation
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13. Compound ID: 9537
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a-D-GlcpN-(1-7)-L-gro-a-D-manHepp-(1-7)-+
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a-D-Galp-(1-2)-a-D-Galp-(1-2)-+ | P---P--4)-+ a-Kdop-(2-4)-+
| | | |
a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-3)-b-D-Glcp-(1-3)-a-D-Glcp-(1-3)-a-D-Glcp-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-a-Kdop-(2--/lipid A/
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P-4)-+ |
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Structure type: oligomer
Aglycon: lipid A
Compound class: LPS, core oligosaccharide with two O-units
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130670,IEDB_131186,IEDB_133751,IEDB_135818,IEDB_136906,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_153543,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_192,SB_7
The structure is contained in the following publication(s):
- Article ID: 4046
Kilar A, Dornyei A, Bui A, Szabo Z, Kocsis B, Kilar F "Structural variability of endotoxins from R-type isogenic mutants of Shigella sonnei" -
Journal of Mass Spectrometry 46(1) (2011) 61-70
The structural variations in the rough-type endotoxins [lipopolysaccharides (LPSs)] of Shigella sonnei mutant strains (S. sonnei phase II-4303, R41, 562H and 4350) were investigated by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem MS. A series of S. sonnei mutants had previously been the subject of analytical studies on the biosynthesis of heptose components in the core oligosaccharide region of LPSs. This study gives a complete overview on the structures of the full core and lipid A of S. sonnei mutant strains by MS. We found that the LPSs of the isogenic rough mutants were formed in a step-like manner containing 0:1:2:3 heptose in the deep core region of 4350, 562H, R41 and 4303, respectively, and the longest LPS from the mutant S. sonnei 4303 contained also five hexoses. The structural variations in the lipid A moiety and in the oligosaccharide part of the intact LPS were followed by MALDI-TOF-MS/MS. For the dissolution and the ionization of the samples, 2,5-dihydroxybenzoic acid in citric acid solution was applied as matrix. The detailed evaluation of the mass spectra indicates heterogeneity in the lipid part due to the differences in the phosphate and fatty acid composition.
lipopolysaccharide structure, MALDI-TOF/TOF-MS, Shigella sonnei rough mutant, MALDI-TOF-MS
NCBI PubMed ID: 21184397Journal NLM ID: 9504818Publisher: Chichester, UK: Wiley
Correspondence: erenc.kilar@aok.pte.hu
Institutions: Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pecs, Ifjusag utja 6, H-7624 Pecs, Hungary
Methods: MS/MS, MALDI-TOF MS
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14. Compound ID: 10680
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a-D-GalpN-(1-4)-a-D-GalpA-(1-7)-L-gro-a-D-manHepp-(1-7)-+ b-D-Galp-(1-4)-+
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a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-b-D-GlcpNAc-(1-6)-a-D-GlcpN-(1-4)-a-D-GalpA-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdo
|
b-D-Glcp-(1-2)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130670,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 4359
Nazarenko EL, Crawford RJ, Ivanova EP "The structural diversity of carbohydrate antigens of selected Gram-negative marine bacteria" -
Marine Drugs 9(10) (2011) 1914-1954
Marine microorganisms have evolved for millions of years to survive in the environments characterized by one or more extreme physical or chemical parameters, e.g., high pressure, low temperature or high salinity. Marine bacteria have the ability to produce a range of biologically active molecules, such as antibiotics, toxins and antitoxins, antitumor and antimicrobial agents, and as a result, they have been a topic of research interest for many years. Among these biologically active molecules, the carbohydrate antigens, lipopolysaccharides (LPSs, O-antigens) found in cell walls of gram-negative marine bacteria, show great potential as candidates in the development of drugs to prevent septic shock due to their low virulence. The structural diversity of LPSs is thought to be a reflection of the ability for these bacteria to adapt to an array of habitats, protecting the cell from being compromised by exposure to harsh environmental stress factors. Over the last few years, the variety of structures of core oligosaccharides and O-specific polysaccharides from LPSs of marine microrganisms has been discovered. In this review, we discuss the most recently encountered structures that have been identified from bacteria belonging to the genera Aeromonas, Alteromonas, Idiomarina, Microbulbifer, Pseudoalteromonas, Plesiomonas and Shewanella of the Gammaproteobacteria phylum; Sulfitobacter and Loktanella of the Alphaproteobacteria phylum and to the genera Arenibacter, Cellulophaga, Chryseobacterium, Flavobacterium, Flexibacter of the Cytophaga-Flavobacterium-Bacteroides phylum. Particular attention is paid to the particular chemical features of the LPSs, such as the monosaccharide type, non-sugar substituents and phosphate groups, together with some of the typifying traits of LPSs obtained from marine bacteria. A possible correlation is then made between such features and the environmental adaptations undertaken by marine bacteria.
O-specific polysaccharides, carbohydrate antigens, marine microorganisms
NCBI PubMed ID: 22073003Publication DOI: 10.3390/md9101914Journal NLM ID: 101213729Publisher: Basel, Switzerland: Molecular Diversity Preservation International
Correspondence: elnaz@piboc.dvo.ru
Institutions: Pacific Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok 690022, Russia, Faculty of Life and Social Sciences, Swinburne University of Technology, PO Box 218, Hawthorn, Victoria 3122, Australia
Methods: 13C NMR, 1H NMR, methylation, periodate oxidation, NMR-2D, FAB-MS, partial acid hydrolysis, NMR, HF solvolysis, sugar analysis, 31P NMR, ESI-MS, acid hydrolysis, mild acid hydrolysis, HPAEC, ESI-ICR-MS, Smith degradation, chemical methods, MALDI-TOF MS, MS, de-O-acetylation, NMR-1D, GPC, alkaline hydrolysis, CE-ESI-MS, CE-MS, hydrazinolysis
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15. Compound ID: 11337
|
?%a-D-GlcpN-(1-4)-a-D-GalpA-(1-7)-L-gro-a-D-manHepp-(1-7)-+ b-D-Galp-(1-4)-+
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a-L-AltpNAcA-(1-3)-b-D-FucpNAc4N-(1-4)-b-D-GlcpNAc-(1-6)-a-D-GlcpN-(1-4)-a-D-GalpA-(1-3)-L-gro-a-D-manHepp-(1-3)-L-gro-a-D-manHepp-(1-5)-Kdop
|
?%b-D-Glcp-(1-2)-+ |
Show graphically |
Structure type: oligomer
Compound class: core oligosaccharide with O-unit
Contained glycoepitopes: IEDB_130650,IEDB_130670,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_140088,IEDB_141794,IEDB_141807,IEDB_142345,IEDB_142488,IEDB_146664,IEDB_151531,IEDB_190606,IEDB_2189047,IEDB_226811,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 4570
Maciejewska A, Lukasiewicz J, Kaszowska M, Man-Kupisinska A, Jachymek W, Lugowski C "Core oligosaccharide of Plesiomonas shigelloides PCM 2231 (Serotype O17) lipopolysaccharide--structural and serological analysis" -
Marine Drugs 11(2) (2013) 440-454
The herein presented complete structure of the core oligosaccharide of lipopolysaccharide (LPS) P. shigelloides Polish Collection of Microorganisms (PCM) 2231 (serotype O17) was investigated by (1)H, (13)C NMR spectroscopy, mass spectrometry, chemical analyses and serological methods. The core oligosaccharide is composed of an undecasaccharide, which represents the second core type identified for P. shigelloides serotype O17 LPS. This structure is similar to that of the core oligosaccharide of P. shigelloides strains 302-73 (serotype O1) and 7-63 (serotype O17) and differs from these only by one sugar residue. Serological screening of 55 strains of P. shigelloides with the use of serum against identified core oligosaccharide conjugated with bovine serum albumin (BSA) indicated the presence of similar structures in the LPS core region of 28 O-serotypes. This observation suggests that the core oligosaccharide structure present in strain PCM 2231 could be the most common type among P. shigelloides lipopolysaccharides.
Lipopolysaccharide, core oligosaccharide, endotoxin, Plesiomonas shigelloides
NCBI PubMed ID: 23389090Publication DOI: 10.3390/md11020440Journal NLM ID: 101213729Publisher: Basel, Switzerland: Molecular Diversity Preservation International
Correspondence: aniaaugustyniuk@iitd.pan.wroc.pl
Institutions: Department of Immunochemistry, Ludwik Hirszfeld Institute Immunology and Experimental Therapy, Polish Academy of Sciences, Rudolfa Wroclaw, Poland
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, SDS-PAGE, sugar analysis, mild acid hydrolysis, MALDI-TOF MS, NMR-1D, serological methods, immunoblotting, mild acid hydrolysismethylation
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