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Structure type: oligomer
Compound class: O-polysaccharide, O-antigen

• Article ID: 2193
  Vinogradov EV, Shashkov AS, Knirel YA, Kochetkov NK, Tochtamysheva NV, Averin SF, Goncharova OV, Khlebnikov VS "Structure of the O-antigen of Francisella tularensis strain 15" - Carbohydrate Research 214 (1991) 289-297
  

  The O-specific polysaccharide, obtained by mild acid degradation of the lipopolysaccharide of Francisella tularensis strain 15, contained 2-acetamido-2,6-dideoxy-D-glucose (D-QuiNAc), 4,6-dideoxy-4-formamido-D-glucose (D-Qui4NFm), and 2-acetamido-2-deoxy-D-galacturonamide (D-GalNAcAN) in the ratios 1:1:2. Tri- and tetra-saccharide fragments were obtained on treatment of the polysaccharide with anhydrous hydrogen fluoride and partial hydrolysis with 0.1 M hydrochloric acid, respectively. On the basis of 1H- and 13C-n.m.r. spectroscopy of the polysaccharide and the saccharides, it was concluded that the O-antigen had the structure: →4)-α-D-GalpNAcAN-(1→4)-α-D-GalpNAcAN-(1→3)-β-D-QuipNAc-(1→2)-β-D-Quip4NFm-(1→. This O-antigen is related in structure to those of Pseudomonas aeruginosa O6, immunotype 1, and IID 1008, and Shigella dysenteriae type 7.

  NCBI PubMed ID: 1769021
  Publication DOI: 10.1016/0008-6215(91)80036-m
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow
  Methods: 13C NMR, 1H NMR, partial acid hydrolysis, HF solvolysis, sugar analysis, GLC, deamination, GPC, NaBH4 reduction
  
   
  
  Francisella tularensis bv. palaearctica, Francisella tularensis B, Francisella tularensis 15
  CSDB ID 2659 (all data & tools)

Show legend Show as text

Structure type: oligomer
Compound class: O-polysaccharide, O-antigen

• Article ID: 2193
  Vinogradov EV, Shashkov AS, Knirel YA, Kochetkov NK, Tochtamysheva NV, Averin SF, Goncharova OV, Khlebnikov VS "Structure of the O-antigen of Francisella tularensis strain 15" - Carbohydrate Research 214 (1991) 289-297
  

  The O-specific polysaccharide, obtained by mild acid degradation of the lipopolysaccharide of Francisella tularensis strain 15, contained 2-acetamido-2,6-dideoxy-D-glucose (D-QuiNAc), 4,6-dideoxy-4-formamido-D-glucose (D-Qui4NFm), and 2-acetamido-2-deoxy-D-galacturonamide (D-GalNAcAN) in the ratios 1:1:2. Tri- and tetra-saccharide fragments were obtained on treatment of the polysaccharide with anhydrous hydrogen fluoride and partial hydrolysis with 0.1 M hydrochloric acid, respectively. On the basis of 1H- and 13C-n.m.r. spectroscopy of the polysaccharide and the saccharides, it was concluded that the O-antigen had the structure: →4)-α-D-GalpNAcAN-(1→4)-α-D-GalpNAcAN-(1→3)-β-D-QuipNAc-(1→2)-β-D-Quip4NFm-(1→. This O-antigen is related in structure to those of Pseudomonas aeruginosa O6, immunotype 1, and IID 1008, and Shigella dysenteriae type 7.

  NCBI PubMed ID: 1769021
  Publication DOI: 10.1016/0008-6215(91)80036-m
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: N. D. Zelinsky Institute of Organic Chemistry, Academy of Sciences of the U.S.S.R., Moscow
  Methods: 13C NMR, 1H NMR, partial acid hydrolysis, HF solvolysis, sugar analysis, GLC, deamination, GPC, NaBH4 reduction
  
   
  
  Francisella tularensis bv. palaearctica, Francisella tularensis B, Francisella tularensis 15
  CSDB ID 2660 (all data & tools)

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Structure type: oligomer
Contained glycoepitopes: IEDB_130648,IEDB_137473,IEDB_1391961,IEDB_141584,IEDB_885822

• Article ID: 4090
  Perepelov AV, Kocharova NA, Knirel YA, Jansson P, Weintraub A "Structure of the O-polysaccharide of Vibrio cholerae O43 containing a new monosaccharide derivative, 4-(N-acetyl-L-allothreonyl)amino-4,6-dideoxy-D-glucose" - Carbohydrate Research 346(3) (2011) 430-433
  

  The O-polysaccharide of Vibrio cholerae O43 was studied using chemical analyses, triflic acid solvolysis and 2D NMR spectroscopy, including (1)H/(1)H COSY, TOCSY, NOESY and (1)H/(13)C gradient-selected HSQC experiments. The following structure of the tetrasaccharide repeating unit of the polysaccharide was established: →3)-β-D-Quip4NAcyl-(1→3)-α-D-GalpNAcA-(1→4)-α-D-GalpNAc-(1→3)-α-D-QuipNAc-(1→ where D-QuiNAc stands for 2-acetamido-2,6-dideoxy-D-glucose, D-Qui4NAcyl for 4-(N-acetyl-L-allothreonyl)amino-4,6-dideoxy-D-glucose and D-GalNAcA for 2-acetamido-2-deoxy-D-galacturonic acid.

  Lipopolysaccharide, bacterial polysaccharide structure, Vibrio cholerae, 4-amino-4, O-antigen gene cluster, L-allothreonine, 6-D-dideoxy-D-glucose

  NCBI PubMed ID: 21232736
  Publication DOI: 10.1016/j.carres.2010.12.009
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Correspondence: perepel@ioc.ac.ru
  Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
  Methods: 13C NMR, 1H NMR, NMR-2D, DNA sequencing, sugar analysis, GLC, triflic acid solvolysis, delipidation, function analysis of gene clusters
  
   
  
  Vibrio cholerae O43
  CSDB ID 26311 (all data & tools)