Found 28 structures.
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1. Compound ID: 703
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a-D-Glcp-(1-3)-a-D-Manp-(1-6)-+ a-Kdop-(2-4)-+
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a-D-Glcp-(1-2)-a-D-Glcp-(1-3)-a-D-Galp-(1-2)-a-D-Manp-(1-4)-a-D-Manp-(1-5)-a-Kdop-(2-6)-D-2,5anhMan-ol
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a-Kdop-(2-3)-+ |
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Structure type: oligomer
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_130701,IEDB_136906,IEDB_137472,IEDB_140116,IEDB_141793,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_153220,IEDB_190606,IEDB_232584,IEDB_983930,IEDB_983931,SB_192,SB_198,SB_44,SB_67,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 187
Vinogradov E, Petersen BO, Sadovskaya I, Jabbouri S, Duus J, Helander IM "Structure of the exceptionally large nonrepetitive carbohydrate backbone of the lipopolysaccharide of Pectinatus frisingensis strain VTT E-82164" -
European Journal of Biochemistry 270(14) (2003) 3036-3046
The structures of the oligosaccharides obtained after acetic acid hydrolysis and alkaline deacylation of the rough-type lipopolysaccharide (LPS) from Pectinatus frisingensis strain VTT E-82164 were analysed using NMR spectroscopy, MS and chemical methods. The LPS contains two major structural variants, differing by a decasaccharide fragment, and some minor variants lacking the terminal glucose residue. The largest structure of the carbohydrate backbone of the LPS that could be deduced from experimental results consists of 25 monosaccharides (including the previously found Arap4NP residue in lipid A) arranged in a well-defined nonrepetitive structure: We presume that the shorter variant with R1 = H represents the core-lipid A part of the LPS, and the additional fragment is present instead of the O-specific polysaccharide. Structures of this type have not been previously described. Analysis of the deacylation products obtained from the LPS of the smooth strain, VTT E-79100T, showed that it contains a very similar core but with one different glycosidic linkage.
Lipopolysaccharide, structure, core, strain, carbohydrate, lipid A, backbone, Pectinatus
NCBI PubMed ID: 12846837Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Correspondence: evguenii.vinogradov@nrc-cnrc.gc.ca
Institutions: Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada, Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark, Laboratoire de Recherche sur les Biomateriaux et Biotechnologies, Universite de Littoral-Cote d'Opale, Bassin Napoleon BP 120, Boulogne-sur-mer, France, Department of Applied Chemistry and Microbiology, Division of Microbiology, University of Helsinki, Finland
Methods: NMR-2D, NMR, chemical methods, MS
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2. Compound ID: 1403
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b-D-Galp-(1-4)-b-D-Glcp-(1-6)-+
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a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-D-2,5anhMan-ol |
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Structure type: oligomer
Trivial name: GBSIII-PS
Contained glycoepitopes: IEDB_136044,IEDB_136794,IEDB_137472,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
The structure is contained in the following publication(s):
- Article ID: 436
Zou W, Laferriere CA, Jennings HJ "Oligosaccharide fragments of the type III group B streptococcal polysaccharide derived from S-pneumoniae type 14 capsular polysaccharide by a chemoenzymatic method" -
Carbohydrate Research 309(3) (1998) 297-301
Partial N-deacetylation fo the GlcNAc residues in S. pneumoniae type 14 capsular polysaccharide (Pn14-PS) backbone was achieved by treatment with base, and the product was subsequently enzymatically sialylated at the 3-O-positions of the terminal galactose residues. The resultant, partially N-deacetylated type III Group B streptococcus capsular polysaccharide (GBSIII-PS) was subjected to nitrous acid deamination, which resulted in the degradation of GBSIII-PS polysaccharide into oligosaccharides containing increasing numbers of the identical repeating units. The oligosaccharides were then separated by passage through a Superdex 30 column and characterized by ESIMS and NMR spectroscopic analysis
Oligosaccharides, capsular polysaccharide, type III group B streptococcus
NCBI PubMed ID: 9742691Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: harry.jnnings@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6
Methods: 1H NMR, ESI-MS, deamination
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3. Compound ID: 1404
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b-D-Galp-(1-4)-b-D-Glcp-(1-6)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+
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a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-D-2,5anhMan-ol |
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Structure type: oligomer
Trivial name: GBSIII-PS
Contained glycoepitopes: IEDB_136044,IEDB_136794,IEDB_137472,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
The structure is contained in the following publication(s):
- Article ID: 436
Zou W, Laferriere CA, Jennings HJ "Oligosaccharide fragments of the type III group B streptococcal polysaccharide derived from S-pneumoniae type 14 capsular polysaccharide by a chemoenzymatic method" -
Carbohydrate Research 309(3) (1998) 297-301
Partial N-deacetylation fo the GlcNAc residues in S. pneumoniae type 14 capsular polysaccharide (Pn14-PS) backbone was achieved by treatment with base, and the product was subsequently enzymatically sialylated at the 3-O-positions of the terminal galactose residues. The resultant, partially N-deacetylated type III Group B streptococcus capsular polysaccharide (GBSIII-PS) was subjected to nitrous acid deamination, which resulted in the degradation of GBSIII-PS polysaccharide into oligosaccharides containing increasing numbers of the identical repeating units. The oligosaccharides were then separated by passage through a Superdex 30 column and characterized by ESIMS and NMR spectroscopic analysis
Oligosaccharides, capsular polysaccharide, type III group B streptococcus
NCBI PubMed ID: 9742691Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: harry.jnnings@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6
Methods: 1H NMR, ESI-MS, deamination
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4. Compound ID: 1405
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b-D-Galp-(1-4)-b-D-Glcp-(1-6)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+
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a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-D-2,5anhMan-ol |
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Structure type: oligomer
Trivial name: GBSIII-PS
Contained glycoepitopes: IEDB_136044,IEDB_136794,IEDB_137472,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
The structure is contained in the following publication(s):
- Article ID: 436
Zou W, Laferriere CA, Jennings HJ "Oligosaccharide fragments of the type III group B streptococcal polysaccharide derived from S-pneumoniae type 14 capsular polysaccharide by a chemoenzymatic method" -
Carbohydrate Research 309(3) (1998) 297-301
Partial N-deacetylation fo the GlcNAc residues in S. pneumoniae type 14 capsular polysaccharide (Pn14-PS) backbone was achieved by treatment with base, and the product was subsequently enzymatically sialylated at the 3-O-positions of the terminal galactose residues. The resultant, partially N-deacetylated type III Group B streptococcus capsular polysaccharide (GBSIII-PS) was subjected to nitrous acid deamination, which resulted in the degradation of GBSIII-PS polysaccharide into oligosaccharides containing increasing numbers of the identical repeating units. The oligosaccharides were then separated by passage through a Superdex 30 column and characterized by ESIMS and NMR spectroscopic analysis
Oligosaccharides, capsular polysaccharide, type III group B streptococcus
NCBI PubMed ID: 9742691Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: harry.jnnings@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6
Methods: 1H NMR, ESI-MS, deamination
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5. Compound ID: 1406
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b-D-Galp-(1-4)-b-D-Glcp-(1-6)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+
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a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-D-2,5anhMan-ol |
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Structure type: oligomer
Trivial name: GBSIII-PS
Contained glycoepitopes: IEDB_136044,IEDB_136794,IEDB_137472,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
The structure is contained in the following publication(s):
- Article ID: 436
Zou W, Laferriere CA, Jennings HJ "Oligosaccharide fragments of the type III group B streptococcal polysaccharide derived from S-pneumoniae type 14 capsular polysaccharide by a chemoenzymatic method" -
Carbohydrate Research 309(3) (1998) 297-301
Partial N-deacetylation fo the GlcNAc residues in S. pneumoniae type 14 capsular polysaccharide (Pn14-PS) backbone was achieved by treatment with base, and the product was subsequently enzymatically sialylated at the 3-O-positions of the terminal galactose residues. The resultant, partially N-deacetylated type III Group B streptococcus capsular polysaccharide (GBSIII-PS) was subjected to nitrous acid deamination, which resulted in the degradation of GBSIII-PS polysaccharide into oligosaccharides containing increasing numbers of the identical repeating units. The oligosaccharides were then separated by passage through a Superdex 30 column and characterized by ESIMS and NMR spectroscopic analysis
Oligosaccharides, capsular polysaccharide, type III group B streptococcus
NCBI PubMed ID: 9742691Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: harry.jnnings@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6
Methods: 1H NMR, ESI-MS, deamination
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6. Compound ID: 1407
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b-D-Galp-(1-4)-b-D-Glcp-(1-6)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+
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a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-D-2,5anhMan-ol |
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Structure type: oligomer
Trivial name: GBSIII-PS
Contained glycoepitopes: IEDB_136044,IEDB_136794,IEDB_137472,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
The structure is contained in the following publication(s):
- Article ID: 436
Zou W, Laferriere CA, Jennings HJ "Oligosaccharide fragments of the type III group B streptococcal polysaccharide derived from S-pneumoniae type 14 capsular polysaccharide by a chemoenzymatic method" -
Carbohydrate Research 309(3) (1998) 297-301
Partial N-deacetylation fo the GlcNAc residues in S. pneumoniae type 14 capsular polysaccharide (Pn14-PS) backbone was achieved by treatment with base, and the product was subsequently enzymatically sialylated at the 3-O-positions of the terminal galactose residues. The resultant, partially N-deacetylated type III Group B streptococcus capsular polysaccharide (GBSIII-PS) was subjected to nitrous acid deamination, which resulted in the degradation of GBSIII-PS polysaccharide into oligosaccharides containing increasing numbers of the identical repeating units. The oligosaccharides were then separated by passage through a Superdex 30 column and characterized by ESIMS and NMR spectroscopic analysis
Oligosaccharides, capsular polysaccharide, type III group B streptococcus
NCBI PubMed ID: 9742691Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: harry.jnnings@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6
Methods: 1H NMR, ESI-MS, deamination
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7. Compound ID: 1408
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b-D-Galp-(1-4)-b-D-Glcp-(1-6)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+ a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-+
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a-Neup5Ac-(2-3)-b-D-Galp-(1-4)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-b-D-GlcpN-(1-3)-b-D-Galp-(1-4)-b-D-Glcp-(1-6)-D-2,5anhMan-ol |
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Structure type: oligomer
Trivial name: GBSIII-PS
Contained glycoepitopes: IEDB_136044,IEDB_136794,IEDB_137472,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_190606,IEDB_983931,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88
The structure is contained in the following publication(s):
- Article ID: 436
Zou W, Laferriere CA, Jennings HJ "Oligosaccharide fragments of the type III group B streptococcal polysaccharide derived from S-pneumoniae type 14 capsular polysaccharide by a chemoenzymatic method" -
Carbohydrate Research 309(3) (1998) 297-301
Partial N-deacetylation fo the GlcNAc residues in S. pneumoniae type 14 capsular polysaccharide (Pn14-PS) backbone was achieved by treatment with base, and the product was subsequently enzymatically sialylated at the 3-O-positions of the terminal galactose residues. The resultant, partially N-deacetylated type III Group B streptococcus capsular polysaccharide (GBSIII-PS) was subjected to nitrous acid deamination, which resulted in the degradation of GBSIII-PS polysaccharide into oligosaccharides containing increasing numbers of the identical repeating units. The oligosaccharides were then separated by passage through a Superdex 30 column and characterized by ESIMS and NMR spectroscopic analysis
Oligosaccharides, capsular polysaccharide, type III group B streptococcus
NCBI PubMed ID: 9742691Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: harry.jnnings@nrc.ca
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario, Canada K1A 0R6
Methods: 1H NMR, ESI-MS, deamination
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8. Compound ID: 2110
Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_133754,IEDB_136105,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 692
Haseley SR, Wilkinson SG "Structure of the O-7 antigen from Acinetobacter baumannii" -
Carbohydrate Research 306 (1998) 257-263
The polymeric O-antigen was isolated from the lipopolysaccharide of the reference of the reference strain for Acinetobacter baumannii serogroup O-7. Both the lipopolysaccharide and the isolated polymer reacted with the homologous antiserum. Monosaccharide analyses and NMR spectra showed that the polymer had a hexasaccharide repeating unit constructed from residues of L-rhamnose (4) and N-acetyl-D-glucosamine (2). The following structure for the repeating unit was established by means of detailed interpretation of the NMR spectra, methylation analysis, and chemical degradations. The tetrasaccharide backbone is identical to that for the O-10 antigen of A. baumannii, which has α-D-ManpNAc as the lateral substituent in place of the disaccharide present in the O-7 antigen. [formula: see text]
Lipopolysaccharide, antigen, LPS, structure, O-antigen, Acinetobacter, Acinetobacter baumannii, L-rhamnose, O-7 antigen
NCBI PubMed ID: 9691449Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: s.g.wilkinson@chem.hull.ac.uk
Institutions: School of Chemistry, University of Hull, UK
Methods: methylation, NMR, Smith degradation, de-N-acetylation
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9. Compound ID: 2121
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a-L-Arap-(1-4)-+
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a-D-GalpA-(1-2)-a-D-GalpA-(1-3)-+ |
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S-Lac-(2-4)-b-D-Manp-(1-4)-b-D-Manp-(1-3)-b-D-Glcp-(1-4)-a-L-Fucp-(1-3)-D-2,5anhMan-ol |
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Structure type: oligomer
Compound class: EPS
Contained glycoepitopes: IEDB_136045,IEDB_137485,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_146664,IEDB_152206,IEDB_152214,IEDB_174333,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_72,SB_86
The structure is contained in the following publication(s):
- Article ID: 636
Garozzo D, Impallomeni G, Spina E, Sturiale L "The structure of the exocellular polysaccharide from the cyanobacterium Cyanospira capsulata" -
Carbohydrate Research 307(1-2) (1998) 113-124
The exocellular polysaccharide produced by the cyanobacterium Cyanospira capsulata has been subjected to partial acid hydrolysis and N-deacetylation-nitrous acid deamination. The oligosaccharides released have been isolated by weak anion exchange and aqueous size exclusion chromatography, and characterized by a combination of 1D and 2D nuclear magnetic resonance spectroscopy, mass spectrometry, sugar composition and linkage analyses. The polysaccharide has an octasaccharide repeating unit with the following structure: [formula: see text]
structure, exopolysaccharide, Cyanospira capsulata, 4-O-(1-carboxyethyl)mannose, blue-green algae, cyanobacterium
NCBI PubMed ID: 9658567Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: gimpa@dipchi.unict.it
Institutions: Istituto per la Chimica e la Tecnologia dei Materiali Polimerici, Consiglio Nazionale delle Ricerche, Catania, Italy, Dipartimento di Scienze Chimiche, Universita di Catania, Viale A. Doria 6, 95125 Catania, Italy
Methods: NMR-2D, partial acid hydrolysis, NMR, MS, de-N-acetylation/deamination, HPAEC-PAD
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10. Compound ID: 2122
Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_151528,IEDB_190606,SB_7
The structure is contained in the following publication(s):
- Article ID: 686
Haseley SR, Holst O, Brade H "Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 90 belonging to DNA group 10" -
European Journal of Biochemistry 245(2) (1997) 470-476
Water-soluble lipopolysaccharide (phenol/water extraction) isolated from Acinetobacter strain 90, which belongs to DNA group 10, was hydrolysed with 1% acetic acid, ultracentrifuged, and water-soluble products finally eluted from a Sephadex G-50 column. The major fraction, a polysaccharide, contained D-Gal, D-GlcNAc, D-GalNAc, and 4,6-dideoxy-4-[(R)-3-hydroxybutyramido]-D-galactose (Fuc4NBuOH). The polysaccharide was characterised by means of monosaccharide analyses, Smith-degradation, N-deacetylation/deamination, and NMR studies, and was shown to have a branched pentasaccharide repeating unit. [structure in text] This structure was specifically recognised in western blots and enzyme immunoassays by polyclonal rabbit antisera.
Lipopolysaccharide, O-antigen, Acinetobacter
NCBI PubMed ID: 9151981Journal NLM ID: 0107600Publisher: Oxford, UK: Blackwell Science Ltd. on behalf of the Federation of European Biochemical Societies
Institutions: Division of Medical and Biochemical Microbiology, Research Centre Borstel, Centre for Medicine and Biosciences, Germany.
Methods: NMR, Smith degradation, de-N-acetylation/deamination
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11. Compound ID: 4222
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b-D-ManpNAc3NAmA-(1-4)-b-D-ManpNAc3NAmA-(1-3)-a-D-FucpNAc-(1-4)-b-D-ManpNAc3NAmA-(1-4)-b-D-ManpNAc3NAmA-(1-3)-b-D-FucpNAc-(1-6)-D-2,5anhMan-ol |
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Structure type: oligomer
Compound class: O-polysaccharide
The structure is contained in the following publication(s):
- Article ID: 1576
Vinogradov E, Caroff M "Structure of the Bordetella trematum LPS O-chain subunit" -
FEBS Letters 579(1) (2005) 18-24
Analysis of the O-chain subunit of the lipopolysaccharide (LPS, endotoxin) isolated from Bordetella trematum, a recently identified human pathogen, was undertaken. The polysaccharide (PS) moiety was shown to contain only two O-chain subunits, which differed in the anomeric bond of their first sugar. A trisaccharide fragment resulting from the cleavage of a FucNAc glycosidic bond was isolated after treatment of the PS with anhydrous HF. Nitrous deamination of the LPS led to the release of the following heptasaccharide corresponding to two trisaccharide subunits linked to an anhydromannitol residue. β-ManNAc3NAmA-(1-4)-β-ManNAc3NAmA-(1-3)-α-FucNAc-(1-4)-β-ManN Ac3NAmA-(1-4)-β-ManNAc3NAmA-(1-3)-β-FucNAc-(1-6)-2,5-anhManol
Lipopolysaccharide, LPS, structure, human, polysaccharide, trisaccharide, analysis, linked, Research, Bordetella, endotoxin, biological, sugar, release, O-chain, treatment, fragment, pathogen, deamination, heptasaccharide, cleavage, anomeric, HF, glycosidic bond
NCBI PubMed ID: 15620685Publication DOI: 10.1016/j.febslet.2004.11.040Journal NLM ID: 0155157Publisher: Elsevier
Institutions: Institute for Biological Sciences, National Research Council, 100 Sussex Dr., Ottawa, ON, Canada K1A 0R6
Methods: NMR, HF solvolysis, TLC, MS, nitrous deamination
- Article ID: 3821
Banoub JH, El Aneed A, Cohen AM, Joly N "Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry" -
Mass Spectrometry Reviews 29(4) (2010) 606-650
Mass spectrometric studies are now playing a leading role in the elucidation of lipopolysaccharide (LPS) structures through the characterization of antigenic polysaccharides, core oligosaccharides and lipid A components including LPS genetic modifications. The conventional MS and MS/MS analyses together with CID fragmentation provide additional structural information complementary to the previous analytical experiments, and thus contribute to an integrated strategy for the simultaneous characterization and correct sequencing of the carbohydrate moiety.
LPS, O-antigen, lipid A, core oligosaccharide, MS and MS/MS analyses
NCBI PubMed ID: 20589944Publication DOI: 10.1002/mas.20258Journal NLM ID: 8219702Publisher: Wiley
Correspondence: joe.banoub@dfo-mpo.gc.ca
Institutions: Fisheries and Oceans Canada, Science Branch, Special Projects, P.O. Box 5667, St. John's, Newfoundland, Canada A1C 5X1, Department of Chemistry, Memorial University of Newfoundland, St. John's, Newfoundland, Canada A1B 3V6, College of Pharmacy and Nutrition, University of Saskatchewan, Thorvaldson Building, 110 Science Place, Saskatoon, Saskatchewan, Canada S7N 5C9, Unité de Catalyse et de Chimie du Solide, Site de l'Artois—UMR CNRS 8181, I.U.T. de Béthune, Département Chimie, 1230 rue de l'Université, BP819, 62408 Béthune Cedex, France, Institute for Marine Biosciences Room 219A, (NRC-IMB), National Research Council of Canada, Government of Canada, 1411 Oxford Street, Halifax, NS, Canada B3H 3Z1
Methods: MS/MS, MS
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12. Compound ID: 5013
Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136105,IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_225177,IEDB_885823,IEDB_983930,SB_44,SB_7,SB_72
The structure is contained in the following publication(s):
- Article ID: 1961
Erbing C, Kenne L, Lindberg B, Hammarstrom S "Structure of the O-specific side-chains of the Escherichia coli O75 lipopolysaccharide: a revision" -
Carbohydrate Research 60(2) (1978) 400-403
no abstract available
Publication DOI: 10.1016/S0008-6215(78)80049-4Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Stockholm, Sweden, Department of Immunology, University of Stockholm, Stockholm, Sweden
Methods: 1H NMR, methylation, gel filtration, sugar analysis, acid hydrolysis, GLC, de-N-acetylation/deamination, ion-exchange chromatography, optical rotation measurement
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13. Compound ID: 5677
|
b-D-Manp-(1-3)-+
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b-L-Fucp-(1-2)-a-D-Galp-(1-3)-D-2,5anhMan-ol |
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Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_137485,IEDB_141794,IEDB_142489,IEDB_144562,IEDB_144983,IEDB_151528,IEDB_152206,IEDB_152214,IEDB_190606,IEDB_983930,SB_44,SB_7,SB_72,SB_86
The structure is contained in the following publication(s):
- Article ID: 2472
Bhattacharyya T, Basu S "Determination of specificities of rabbit antisera against the O-antigenic polysaccharide from Escherichia coli O126" -
FEMS Immunology and Medical Microbiology 10 (1994) 19-24
Journal NLM ID: 9315554Publisher: Elsevier
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14. Compound ID: 6102
Structure type: oligomer
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_133754,IEDB_136105,IEDB_225177,IEDB_885823
The structure is contained in the following publication(s):
- Article ID: 2715
Carlin NIA, Bundle DR, Lindberg AA "Characterization of five Shigella flexneri variant Y-specific monoclonal antibodies using defined saccharides and glycoconjugate antigens" -
Journal of Immunology 138 (1987) 4419-4427
The structural domains of the Shigella flexneri variant Y O-antigen epitopes 3,4 have defied definition, despite knowledge of the structure of the linear polysaccharide chain of the LPS molecule. The dual epitope designation of group antigen 3,4 is based on absorption data using polyvalent rabbit antisera. Five monoclonal antibodies specific for the Y antigen, generated after immunization of BALB/c mice or LOU/C rats, were selected on the basis of ELISA by using well-characterized S. flexneri Y LPS and chemically defined glycoconjugates. Chemically defined LPS from all S. flexneri serogroups, synthetic oligosaccharides, and saccharides obtained by phage Sf6-mediated hydrolysis of the O-polysaccharide were used either as free haptens or glycoconjugates in Farr assays and ELISA titrations. Two different patterns of antibody specificities were seen: two monoclonal antibodies had combining sites recognizing the terminal nonreducing end of the O-polysaccharide complementary to the tetrasaccharide repeating unit; and three antibodies bound to intrachain determinants and had larger combining sites, possibly accommodating at least an octasaccharide. The precise specificity of these two general types of antibodies indicate that variant Y polysaccharide generates more than two O-factors.
NCBI PubMed ID: 2438343Journal NLM ID: 2985117RPublisher: Bethesda, MD: American Association of Immunologists
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15. Compound ID: 8497
Structure type: monomer
The structure is contained in the following publication(s):
- Article ID: 3706
Karas M, Russa R "Localization of the attachment site of oligoglucans to Mesorhizobium loti HAMBI 1148 murein" -
Acta Biochimica Polonica 56(1) (2009) 155-160
The location and nature of the linkage between peptidoglycan and oligoglucans in the cell wall of Mesorhizobium loti HAMBI 1148 have been defined by the analysis of nitrous acid deamination of peptidoglycan glucosaminyl residues. The MurNH(2)-Glc(n) fraction was obtained after converting deaminoacylated and N-deacetylated muramyl residues in the cell wall preparation to lactam forms which were stable during subsequent deamination, followed by reduction and opening of the lactams. GC/MS analysis of this material, subjected to partial hydrolysis and reduction or to methanolysis followed by peracetylation, confirmed the presence of glucosyl residues glycosidically attached to muramic acid. The MALDI-TOF spectroscopic analysis of the deaminated material also revealed the presence of [M-H](-) or [M+Na-2H](-) ions representing fragments containing muramic acid with one to three linked glucose residues. The analysis of fully methylated neutral oligosaccharides released from the peptidoglycan with lysozyme followed by borohydride reduction showed the presence of di- and trisaccharides lacking the reducing end.
MALDI-TOF, deamination, murein, Mesorhizobium, oligoglucan
NCBI PubMed ID: 19294234Journal NLM ID: 14520300RPublisher: Panstwowe Wydawnictwo Naukowe
Correspondence: Ryszard.Russa@poczta.umcs.lublin.pl
Institutions: Department of Genetics and Microbiology, Maria Curie-Sklodowska University, Lublin, Poland
Methods: partial acid hydrolysis, GC-MS, sugar analysis, MALDI-TOF MS, de-N-acetylation, alkaline de-N-acylation, enzymatic digestion, lactamization with hydrazine acetate
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