| Enzyme / Gene |
Activity |
Object |
References |
|---|
Enzyme name: HPAT1
Gene name: At5g25265
Gene GenBank ID: 832598  | Synthesized dimer: bLAraf(1-4)Hyp
x?Hyp?)
Donor (ID 34165): bLAraf(1-P-P-5)xXnucU
Acceptor (ID 34166): x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser
Status: evidence in vitro
Confirmation methods: in vitro (microsomal membrane preparation)
ID: 1775 Notes: HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3.
| Organism (ID 9805): Arabidopsis thaliana
Organ/tissue: seedling, root, leaf, stem, flower bud, flower, silique
Full structure (ID 34167):
x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser&highlight=3~4~bLAra) | Ogawa-Ohnishi et al. 2013
DOI: 10.1038/nchembio.1351
|
Synthesized dimer: bLAraf(1-4)Hyp
Donor (ID 34165): bLAraf(1-P-P-5)xXnucU
Acceptor (ID 34168): x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser
Status: evidence in vitro
Confirmation methods: in vitro (microsomal membrane preparation), mutation
ID: 1776 Notes: HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3.
| Organism (ID 9805): Arabidopsis thaliana
Organ/tissue: seedling, root, leaf, stem, flower bud, flower, silique
Full structure (ID 34169):
xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser&highlight=3~4~bLAra) |
Enzyme name: HPAT2
Gene name: At2g25260
Gene GenBank ID: 817064 | Synthesized dimer: bLAraf(1-4)Hyp
Donor (ID 34165): bLAraf(1-P-P-5)xXnucU
Acceptor (ID 34168): x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser
Status: evidence in vitro
Confirmation methods: in vitro (microsomal membrane preparation), mutation
ID: 1777 Notes: HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3.
| Organism (ID 9805): Arabidopsis thaliana
Organ/tissue: seedling, root, leaf, stem, flower bud, flower
Full structure (ID 34169):
|
Enzyme name: HPAT3
Gene name: At5g13500
Gene GenBank ID: 831194 | Synthesized dimer: bLAraf(1-4)Hyp
Donor (ID 34165): bLAraf(1-P-P-5)xXnucU
Acceptor (ID 34168): x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser
Status: evidence in vitro
Confirmation methods: in vitro (microsomal membrane preparation), mutation
ID: 1778 Notes: HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3. HAPT3 contributes
to arabinosylation of CLE peptides.
| Organism (ID 9805): Arabidopsis thaliana
Organ/tissue: seedling, root, leaf, stem, flower bud, flower, silique
Full structure (ID 34169):
|
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
1775 HPAT1 At5g25265 832598 34167 x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser 0 bLAraf(1-4)x?Hyp? 3~4 bLAraf(1-P-P-5)xXnucU x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Ala?(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser in vitro in vitro (microsomal membrane preparation) HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3. 0 0 9805 Arabidopsis thaliana seedling, root, leaf, stem, flower bud, flower, silique Ogawa-Ohnishi et al. 2013 (doi: 10.1038/nchembio.1351)
1776 HPAT1 At5g25265 832598 34169 x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser 0 bLAraf(1-4)x?Hyp? 3~4 bLAraf(1-P-P-5)xXnucU x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser in vitro in vitro (microsomal membrane preparation), mutation HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3. 0 0 9805 Arabidopsis thaliana seedling, root, leaf, stem, flower bud, flower, silique Ogawa-Ohnishi et al. 2013 (doi: 10.1038/nchembio.1351)
1777 HPAT2 At2g25260 817064 34169 x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser 0 bLAraf(1-4)x?Hyp? 3~4 bLAraf(1-P-P-5)xXnucU x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser in vitro in vitro (microsomal membrane preparation), mutation HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3. 0 0 9805 Arabidopsis thaliana seedling, root, leaf, stem, flower bud, flower Ogawa-Ohnishi et al. 2013 (doi: 10.1038/nchembio.1351)
1778 HPAT3 At5g13500 831194 34169 x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)[bLAraf(1-4)]x?Hyp?(1-2)x?Hyp?(1-2)x?Ser 0 bLAraf(1-4)x?Hyp? 3~4 bLAraf(1-P-P-5)xXnucU x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser(1-2)x?Pro(1-2)xXGly(1-2)x?Val(1-2)x?Hyp?(1-2)x?Hyp?(1-2)x?Ser in vitro in vitro (microsomal membrane preparation), mutation HPAT1, HPAT2 and HPAT3 redundantly contribute to arabinosylation of EXT3. HAPT3 contributes to arabinosylation of CLE peptides. 0 0 9805 Arabidopsis thaliana seedling, root, leaf, stem, flower bud, flower, silique Ogawa-Ohnishi et al. 2013 (doi: 10.1038/nchembio.1351)