| Enzyme / Gene |
Activity |
Object |
References |
Enzyme name: WzY
UniProt ID: P27835*
| Synthesized dimer: aDGalpN(1-2)aDGalp
aDGalp)
Donor (ID 34539): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = 3,7,11,15,19,23,27,31,35,39,43-undecamethyl-tetratetraconta-2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E,42-undecaen-1-ol
Acceptor (ID 34539): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = 3,7,11,15,19,23,27,31,35,39,43-undecamethyl-tetratetraconta-2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E,42-undecaen-1-ol
Status: evidence in vitro
Confirmation methods: in vitro (purified protein)
ID: 2263 Notes: Wzy acts as an O-antigen polymerase. An exemplary structure is shown.
| Organism (ID 2516): Escherichia coli O86:B7
Full structure (ID 34540):
[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = 3,7,11,15,19,23,27,31,35,39,43-undecamethyl-tetratetraconta-2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E,42-undecaen-1-ol&highlight=7~2~aDGalN)
Molecule role: O-antigen | Woodward et al. 2010
DOI: 10.1038/nchembio.351
|
Synthesized dimer: aDGalpN(1-2)aDGalp
aDGalp)
Donor (ID 34541): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = heptaprenol (4cis, 2trans)
Acceptor (ID 34541): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = heptaprenol (4cis, 2trans)
Status: evidence in vitro
Confirmation methods: in vitro (purified protein)
ID: 2264 Notes: Wzy acts as an O-antigen polymerase. An exemplary structure is shown.
| Organism (ID 2516): Escherichia coli O86:B7
Full structure (ID 34542):
[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = heptaprenol (4cis, 2trans)&highlight=7~2~aDGalN)
Molecule role: O-antigen |
Synthesized dimer: aDGalpN(1-2)aDGalp
aDGalp)
Donor (ID 34543): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = pentaprenol (2cis, 2trans)
Acceptor (ID 34543): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = pentaprenol (2cis, 2trans)
Status: evidence in vitro
Confirmation methods: in vitro (purified protein)
ID: 2265 Notes: Wzy acts as an O-antigen polymerase. An exemplary structure is shown.
| Organism (ID 2516): Escherichia coli O86:B7
Full structure (ID 34544):
[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = pentaprenol (2cis, 2trans)&highlight=7~2~aDGalN)
Molecule role: O-antigen |
Synthesized dimer: aDGalpN(1-2)aDGalp
aDGalp)
Donor (ID 34545): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = cis-pentaprenol (4cis, 0trans)
Acceptor (ID 34545): aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = cis-pentaprenol (4cis, 0trans)
Status: evidence in vitro
Confirmation methods: in vitro (purified protein)
ID: 2266 Notes: Wzy acts as an O-antigen polymerase. An exemplary structure is shown.
| Organism (ID 2516): Escherichia coli O86:B7
Full structure (ID 34546):
[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = cis-pentaprenol (4cis, 0trans)&highlight=7~2~aDGalN)
Molecule role: O-antigen |
#GTR ID Enzyme name Enzyme group Enzyme Genbank ID Derived ID mark Enzyme Uniprot ID Derived ID mark CAZY family Gene name Gene Genbank ID Derived ID mark Gene cluster Full structure CSDB structure ID Side product CSDB structure ID Full structure Full structure is fragment (0|1) Synthesized bond Synthesized bond location (from root ~ subst position) Donor structure ID Substrate structure Confirmation status Methods used Notes Molecule role Molecule is a model of role (0|1) Molecule is a precursor of role (0|1) Main CSDB record ID Additional CSDB record IDs CSDB organism IDs Organism names Organs and tissues References (;-separated)
2263 WzY P27835 * 34540 aLFucp(1-2)[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = 3,7,11,15,19,23,27,31,35,39,43-undecamethyl-tetratetraconta-2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E,42-undecaen-1-ol 0 aDGalpN(1-2)aDGalp 7~2 aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = 3,7,11,15,19,23,27,31,35,39,43-undecamethyl-tetratetraconta-2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E,42-undecaen-1-ol aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = 3,7,11,15,19,23,27,31,35,39,43-undecamethyl-tetratetraconta-2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E,42-undecaen-1-ol in vitro in vitro (purified protein) Wzy acts as an O-antigen polymerase. An exemplary structure is shown. O-antigen 0 0 2516 Escherichia coli O86:B7 Woodward et al. 2010 (doi: 10.1038/nchembio.351)
2264 WzY P27835 * 34542 aLFucp(1-2)[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = heptaprenol (4cis, 2trans) 0 aDGalpN(1-2)aDGalp 7~2 aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = heptaprenol (4cis, 2trans) aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = heptaprenol (4cis, 2trans) in vitro in vitro (purified protein) Wzy acts as an O-antigen polymerase. An exemplary structure is shown. O-antigen 0 0 2516 Escherichia coli O86:B7 Woodward et al. 2010 (doi: 10.1038/nchembio.351)
2265 WzY P27835 * 34544 aLFucp(1-2)[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = pentaprenol (2cis, 2trans) 0 aDGalpN(1-2)aDGalp 7~2 aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = pentaprenol (2cis, 2trans) aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = pentaprenol (2cis, 2trans) in vitro in vitro (purified protein) Wzy acts as an O-antigen polymerase. An exemplary structure is shown. O-antigen 0 0 2516 Escherichia coli O86:B7 Woodward et al. 2010 (doi: 10.1038/nchembio.351)
2266 WzY P27835 * 34546 aLFucp(1-2)[aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-2)aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = cis-pentaprenol (4cis, 0trans) 0 aDGalpN(1-2)aDGalp 7~2 aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = cis-pentaprenol (4cis, 0trans) aLFucp(1-2)[aDGalp(1-3)]bDGalp(1-3)[Ac(1-2)]aDGalpN(1-3)[Ac(1-2)]aDGalpN(1-P-P-1)Subst // Subst = cis-pentaprenol (4cis, 0trans) in vitro in vitro (purified protein) Wzy acts as an O-antigen polymerase. An exemplary structure is shown. O-antigen 0 0 2516 Escherichia coli O86:B7 Woodward et al. 2010 (doi: 10.1038/nchembio.351)