Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 15778030Journal NLM ID: 7705469Publisher: Amsterdam; Elsevier
Institutions: Teda School of Biological Sciences and Biotechnology, Nankai University, Teda College, 23 HongDa Street, Teda, Tianjin, China, Tianjin State Laboratory of Microbial Functional Genomics, TEDA College, Nankai University, 23 HongDa Street, Teda, Tianjin, China, Tianjin Biochip Technology Corporation, 23 HongDa Street, Teda, Tianjin, China
Escherichia coli O86 belongs to the enteropathogenic E. coli (EPEC) group, some strains of which are pathogens of humans, wild birds and farm animals. The O-antigen gene cluster of E. coli O86 was amplified by long-range PCR using primers based on the housekeeping genes galF and gnd, and then sequenced. Genes involved in GDP-Fuc and N-acetyl-galactosamine (GalNAc) synthesis and genes encoding glycosyltransferases, O-unit flippase and O-antigen polymerase were identified on the basis of homology. By screening against 186 E. coli and Shigella-type strains, two genes specific to E. coli O86 were identified. A polymerase chain reaction (PCR) assay, based on the specific O-antigen genes identified here, could be used for the rapid detection of E. coli O86 in environmental and clinical samples. The relationship between E. coli O86 and O127 was also determined by comparing the two O-antigen gene clusters
synthesis, clinical, functional, gene, human, PCR, polymerase chain reaction, strain, characterization, O-antigen, O antigen, chain, group, Escherichia, Escherichia coli, animal, cluster, gene cluster, specific, biological, glycosyltransferases, identification, glycosyltransferase, pathogen, pathogens, relationship, enteropathogenic, O-antigen polymerase, biotechnology, homology, reaction, genomics, detection, assay, long range, long-range, environmental, Animals, Humans
Structure type: suggested polymer biological repeating unit
Location inside paper: p.242
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_140125,IEDB_141582,IEDB_141584,IEDB_141794,IEDB_142489,IEDB_143260,IEDB_144562,IEDB_149558,IEDB_150766,IEDB_150948,IEDB_150952,IEDB_151528,IEDB_152212,IEDB_152214,IEDB_153207,IEDB_153553,IEDB_174333,IEDB_190606,IEDB_241096,IEDB_461710,IEDB_461718,IEDB_461719,IEDB_885822,IEDB_918314,SB_148,SB_154,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_7,SB_8,SB_86,SB_87,SB_88
Methods: genetic methods
Biosynthesis and genetic data: genetic data, biosynthetic data
Related record ID(s): 10195, 10220, 10548, 20043, 20120, 20508, 20667, 25273, 108706, 115586
NCBI Taxonomy refs (TaxIDs): 2162909Reference(s) to other database(s): GTC:G75734HT, GlycomeDB:
3571
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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