Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 15686943Journal NLM ID: 9107377Publisher: Elsevier
Institutions: Department of Chemistry, Queen's University, Kingston, Ontario, Canada K7L 3N6
A novel acceptor substrate for galactosyltransferase was synthesized containing GlcNAcα-pyrophosphate, covalently bound to a hydrophobic phenoxyundecyl moiety (GlcNAc α-O-PO(3)-PO(3)-(CH(2))(11)-O-Phenyl). The new substrate was used to develop an assay for a galactosyltransferase activity from Escherichia coli strain VW187 that is involved in lipopolysaccharide synthesis and has not been studied by others. We showed that Gal was transferred from UDP-Gal to the novel acceptor substrate. This was a significant improvement over our previous preliminary assays of the enzyme using endogenous substrate, and showed that these synthetic substrates are useful for assaying enzymes that utilize lipid-bound substrates in O-chain synthesis in Gram-negative bacteria
Lipopolysaccharide, synthesis, chemistry, strain, Escherichia, Escherichia coli, bacteria, activity, identification, Gram-negative bacteria, enzyme, gram negative bacteria, Gram-negative, O-chain, Enzymes, acceptor, galactosyltransferase, hydrophobic, Synthetic, bound, substrate, PDF, assay, lipid-bound
Structure type: polymer chemical repeating unit
Location inside paper: p.1205
Contained glycoepitopes: IEDB_130701,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_1394181,IEDB_141794,IEDB_141807,IEDB_142078,IEDB_144983,IEDB_150899,IEDB_151531,IEDB_152206,IEDB_190606,IEDB_983930,SB_137,SB_165,SB_166,SB_187,SB_195,SB_29,SB_44,SB_67,SB_7,SB_72,SB_88
Methods: chemical synthesis
Enzymes that release or process the structure: galactosyltransferase
Biosynthesis and genetic data: biosynthesis data
Synthetic data: chemical
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G41918HB
Show glycosyltransferases
There is only one chemically distinct structure:
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