Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Sus scrofa
Associated disease: meningitis [ICD11:
1D01 
];
septicemia [ICD11:
MA15.Y ];
inflammatory arthropathies [ICD11:
FA2Z ];
infection due to Streptococcus suis [ICD11:
XN5SE ]
The structure was elucidated in this paperNCBI PubMed ID: 30605786Publication DOI: 10.1016/j.carres.2018.12.009Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: M. Segura <mariela.segura
umontreal.ca>
Institutions: Swine and Poultry Infectious Diseases Research Center, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte St., St-Hyacinthe, Quebec, J2S 2M2, Canada, Canadian Glycomics Network (GlycoNet), University of Alberta, 11227 Saskatchewan Dr., Edmonton, Alberta, T6G 2G2, Canada, National Research Council, 100 Sussex Dr., Ottawa, Ontario, K1A 0R6, Canada, Division of Bacterial and Parasitic Disease, National Institute of Animal Health, National Agriculture and Food Research Organization, 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan, The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan
Streptococcus suis serotypes 7 and 8 are counted among the top six S. suis serotypes causing clinical disease in pigs. Yet, limited information is available on these serotypes. Since S. suis serotyping system is based upon capsular polysaccharide (CPS) antigenicity and the CPS is considered a major virulence factor for encapsulated pathogens, here we determined for the first time the chemical compositions and structures of serotypes 7 and 8 CPSs. Chemical and spectroscopic data gave the following repeating unit sequences: [3)L-Rha(α1-P-2)D-Gal(α1-4)D-GlcA(β1-3)D-FucNAc4N(α1-]n for serotype 7 and [2)L-Rha(α1-P-4)D-ManNAc(β1-4)D-Glc(α1-]n for serotype 8. As serotype 8 CPS is identical to Streptococcus pneumoniae type 19F CPS, dot-blot analyses showed a strong reaction of the 19F polysaccharide with reference anti-S. suis serotype 8 rabbit serum. A correlation between S. suis serotypes 7 and 8 sequences and genes of those serotypes' loci encoding putative glycosyltransferases and polymerases responsible for the biosynthesis of the repeating units was tentatively established. Knowledge of CPS structure and composition will contribute to better dissect the role of this bacterial component in the pathogenesis of the disease caused by S. suis serotypes 7 and 8.
capsular polysaccharide, carbohydrate structure, Serotype 8, Streptococcus suis, Serotype 7
Structure type: polymer chemical repeating unit
Location inside paper: abstract, table 1, p.39, S. suis 7
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_136105,IEDB_136906,IEDB_137472,IEDB_140630,IEDB_141794,IEDB_142345,IEDB_151528,IEDB_190606,IEDB_225177,IEDB_423153,IEDB_885823,SB_7
Methods: 13C NMR, 1H NMR, GLC-MS, gel filtration, NMR-2D, sugar analysis, 31P NMR, acid hydrolysis, Western blotting, bioinformatic analysis
Related record ID(s): 737, 738, 739, 740, 741, 742, 743, 744, 745, 746, 747, 748, 749, 750, 751, 752, 753, 754, 755, 756, 757
NCBI Taxonomy refs (TaxIDs): 1307Reference(s) to other database(s): GTC:G50974VH
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,4,2,0 aLRhap 97.2 68.5 76.7 71.3 71.1 18.1
3,4,2 P
3,4 aDGalp 97.9 74.0 69.3 70.0 71.4 61.5
3 bDGlcpA 105.4 73.4 77.8 77.8 77.1 176.1
2 Ac 175.9 23.2
aDFucpN4N 95.8 49.0 75.8 56.1 63.9 16.5
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,2,0 aLRhap 5.45 4.15 3.87 3.55 3.95 1.34
3,4,2 P
3,4 aDGalp 5.69 4.26 3.90 4.03 3.95 3.69-3.69
3 bDGlcpA 4.62 3.37 3.80 3.80 3.86 -
2 Ac - 2.04
aDFucpN4N 5.10 4.23 4.35 3.90 4.64 1.27
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,4,2,0 aLRhap 97.2/5.45 68.5/4.15 76.7/3.87 71.3/3.55 71.1/3.95 18.1/1.34
3,4,2 P
3,4 aDGalp 97.9/5.69 74.0/4.26 69.3/3.90 70.0/4.03 71.4/3.95 61.5/3.69-3.69
3 bDGlcpA 105.4/4.62 73.4/3.37 77.8/3.80 77.8/3.80 77.1/3.86
2 Ac 23.2/2.04
aDFucpN4N 95.8/5.10 49.0/4.23 75.8/4.35 56.1/3.90 63.9/4.64 16.5/1.27
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,2,0 | aLRhap | 5.45 | 4.15 | 3.87 | 3.55 | 3.95 | 1.34 |
| 3,4,2 | P | |
| 3,4 | aDGalp | 5.69 | 4.26 | 3.90 | 4.03 | 3.95 | 3.69
3.69 |
| 3 | bDGlcpA | 4.62 | 3.37 | 3.80 | 3.80 | 3.86 |
|
| 2 | Ac |
| 2.04 | |
| | aDFucpN4N | 5.10 | 4.23 | 4.35 | 3.90 | 4.64 | 1.27 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,4,2,0 | aLRhap | 97.2 | 68.5 | 76.7 | 71.3 | 71.1 | 18.1 |
| 3,4,2 | P | |
| 3,4 | aDGalp | 97.9 | 74.0 | 69.3 | 70.0 | 71.4 | 61.5 |
| 3 | bDGlcpA | 105.4 | 73.4 | 77.8 | 77.8 | 77.1 | 176.1 |
| 2 | Ac | 175.9 | 23.2 | |
| | aDFucpN4N | 95.8 | 49.0 | 75.8 | 56.1 | 63.9 | 16.5 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
