The bacterium Moraxella bovis is the causative agent of an economically important disease of cattle: Infectious Bovine Keratoconjunctivitis (IBK), otherwise known as pinkeye. Little is known regarding the structure of the carbohydrates produced by M. bovis. The structure of a capsular polysaccharide from M. bovis (strain Mb25) has been determined using NMR and MS analysis. From these data it is concluded that the polysaccharide is composed of the unmodified chondroitin disaccharide repeat unit

NMR, carbohydrates, structure, disease, strain, capsular, polysaccharide, analysis, carbohydrate, capsular polysaccharide, bacteria, Moraxella, identification, Infectious, agent, MS, disaccharide, chondroitin, bovine, cattle, Keratoconjunctivitis

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3	bDGlcpA	107.07	74.76	76.44	82.6	76.44	174.51
2	Ac	177.67	25.09
	bDGalpN	104.03	53.80	82.80	70.37	77.68	63.64


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
3	bDGlcpA	4.56	3.38	3.66	3.82	3.99	-
2	Ac	-	1.99
	bDGalpN	4.50	3.99	3.84	4.09	3.70	3.69


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
3	bDGlcpA	107.07/4.56	74.76/3.38	76.44/3.66	82.6/3.82	76.44/3.99	
2	Ac		25.09/1.99
	bDGalpN	104.03/4.50	53.80/3.99	82.80/3.84	70.37/4.09	77.68/3.70	63.64/3.69

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

*O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H](O)[C@@H](CO)O[C@@H](*)[C@@H]2NC(C)=O)O[C@@H]1C(=O)O

379.318 g/mol (C₁₄H₂₁R₂NO₁₁, R = next and previous repeats, not counted in mol weight)