Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 15713070Publication DOI: 10.1021/ja045021yPublisher: American Chemical Society
Institutions: Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA
Previous study showed that some Gram-negative bacteria possess human blood group activity. Among them, Escherichia coli O86 has high blood group B activity and weak blood group A activity. This is due to the cell surface O-antigen structure, which resembles that of human blood group B antigen. In this study, we sequenced the entire E. coli O86 antigen gene cluster and identified all the genes responsible for O-antigen biosynthesis by sequence comparative analysis. The blood group B-like antigen in E. coli O86 O-polysaccharide was synthesized by sequentially employing three glycosyltransferases identified in the gene cluster. More importantly, we identified a new bacterial glycosyltransferase (WbnI) equivalent to human blood group transferase B (GTB). The enzyme substrate specificity and stepwise enzymatic synthesis of blood group B-like antigen revealed that the biosynthetic pathway of B antigen is essentially the same in E. coli O86 as in humans. This new finding provides a model to study the specificity and structure relationship of blood group transferases and supports the hypothesis of anti-blood group antibody production by bacterial stimulation.
synthesis, antigen, tetrasaccharide, biosynthetic, blood, gene, human, O-antigen, O antigen, group, Escherichia, Escherichia coli, cluster, gene cluster, enzymatic, biochemistry, enzymatic synthesis, blood group
Structure type: suggested polymer biological repeating unit
Location inside paper: p.2040
Compound class: O-polysaccharide
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_130648,IEDB_134627,IEDB_136044,IEDB_136045,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_1391961,IEDB_1391963,IEDB_140125,IEDB_141582,IEDB_141584,IEDB_141794,IEDB_142489,IEDB_143260,IEDB_144562,IEDB_149558,IEDB_150766,IEDB_150948,IEDB_150952,IEDB_151528,IEDB_152212,IEDB_152214,IEDB_153553,IEDB_174333,IEDB_190606,IEDB_241096,IEDB_461710,IEDB_461718,IEDB_461719,IEDB_885822,IEDB_918314,SB_148,SB_154,SB_165,SB_166,SB_187,SB_195,SB_23,SB_24,SB_7,SB_8,SB_86,SB_87,SB_88
Methods: genetic methods
Biological activity: blood group B antigen
Biosynthesis and genetic data: genetic data, biosynthetic data
Related record ID(s): 10035, 10548, 20043, 20120, 20508, 20667, 25273, 108706, 115586
NCBI Taxonomy refs (TaxIDs): 2162909Reference(s) to other database(s): GTC:G13805JB, GlycomeDB:
27658
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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