Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Host organism: Sus scrofa
Associated disease: septicemia [ICD11:
MA15.Y 
];
meningitis [ICD11:
1D01 ];
infection due to Streptococcus suis [ICD11:
XN5SE ]
NCBI PubMed ID: 31500262Publication DOI: 10.3390/pathogens8030139Journal NLM ID: 101596317Publisher: Basel, Switzerland: MDPI AG
Correspondence: M. Segura <mariela.segura
umontreal.ca>
Institutions: Research Group on Infectious Diseases in Production Animals, Faculty of Veterinary Medicine, University of Montreal, St-Hyacinthe, QC J2S 2M2, Canada, Swine and Poultry Infectious Diseases Research Centre, Saint-Hyacinthe, QC J2S 2M2, Canada, Canadian Glycomics Network (GlycoNet), University of Alberta, Edmonton, AB, Canada, Department of Chemistry, Université du Québec à Montréal, Montreal, QC H3C 3P8, Canada
Streptococcus suis serotype 2 is an encapsulated bacterium and an important swine pathogen. Opsonizing antibody responses targeting capsular polysaccharides (CPSs) are protective against extracellular pathogens. To elucidate the protective activity of monoclonal antibodies (mAbs) directed against S. suis serotype 2 CPS, mice were immunized with a serotype 2 CPS-glycoconjugate and three hybridomas were isolated; of which, two were murine IgMs and the other a murine IgG1. Whereas the IgMs (mAbs 9E7 and 13C8) showed different reactivity levels with S. suis serotypes 1, 1/2, 2 and 14, the IgG1 (mAb 16H11) was shown to be serotype 2-specific. All mAbs targeted the sialylated chain of the CPSs. Using an opsonophagocytosis assay, the IgMs were opsonizing towards the S. suis serotypes to which they cross-react, while the IgG1 failed to induce bacterial elimination. In a model of mouse passive immunization followed by a lethal challenge with S. suis serotype 2, the IgG1 and IgM cross-reacting only with serotype 14 (mAb 13C8) failed to protect, while the IgM cross-reacting with serotypes 1, 1/2, and 14 (mAb 9E7) was shown to be protective by limiting bacteremia. These new mAbs show promise as new S. suis diagnostic tools, as well as potential for therapeutic applications.
monoclonal antibody, capsular polysaccharide, Streptococcus suis, Opsonophagocytosis, serotype 2
Structure type: polymer chemical repeating unit
Location inside paper: fig.1, serotype 2
Compound class: CPS, O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_130646,IEDB_130697,IEDB_135813,IEDB_136044,IEDB_136794,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_137776,IEDB_140108,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_146100,IEDB_146664,IEDB_146668,IEDB_149174,IEDB_151528,IEDB_151531,IEDB_158551,IEDB_190606,IEDB_225177,IEDB_548867,IEDB_548870,IEDB_885823,IEDB_983931,SB_126,SB_132,SB_165,SB_166,SB_170,SB_171,SB_172,SB_173,SB_187,SB_192,SB_195,SB_30,SB_7,SB_84,SB_88
Methods: ELISA, Western blotting, biological assays, serological methods, agglutination, statistical analysis, confocal microscopy, opsonophagocytosis assay, monoclonal antibodies, antibody production
Comments, role: S. suis serotype 2 strain S735.
Related record ID(s): 758, 759, 760, 761
NCBI Taxonomy refs (TaxIDs): 1184252Reference(s) to other database(s): GTC:G87088VU, GlycomeDB:
37829
Show glycosyltransferases
There is only one chemically distinct structure:
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