Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11:
XN3LD 
]
The structure was elucidated in this paperNCBI PubMed ID: 34474230Publication DOI: 10.1016/j.jpba.2021.114340Journal NLM ID: 8309336Publisher: London: Elsevier
Correspondence: (J. Yan <zijie1011
yeah.net>
Institutions: Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University), Chongqing 400038, PR China
Burkholderia pseudomallei causes melioidosis - an infectious disease with high mortality. Its varied clinical manifestations and resistance to many antibiotics make it a potential biothreat agent and calls for a robust diagnostic assay and effective vaccines. Bacterial cell surface polysaccharides are considered a valuable target for diagnostics and as protective antigen candidates. This study characterized the structure of polysaccharides of B. pseudomallei clinical strain from Hainan, China. A novel structural domain [→3-(α-D-Manp-1→3-α-D-Manp)2-2Me-α-L-6dTalp-1→] was identified by chemical analysis, gas chromatography-mass spectrometry (GC-MS), and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Immunofluorescence and enzyme-linked immunosorbent assay (ELISA) showed that the serum antibodies against the purified polysaccharide antigen could recognize and bind specifically to B. pseudomallei strains. Additionally, the assays revealed cross-reactivity with polysaccharides from different clinical strains. The polysaccharide antigen also exhibited a strong reaction with the sera from melioidosis patients. Thus, the pentasaccharide repeating unit residue could be a potential candidate antigen for the melioidosis serodiagnosis and vaccine development.
polysaccharide, Burkholderia pseudomallei, Structural characterization, immunological characteristics
Structure type: structural motif or average structure
Location inside paper: p. 114340-6, table 3, BPC006-BPPI-b1 fraction
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, ELISA, anion-exchange chromatography, composition analysis, GPC, UV, extraction, statistical analysis, immunization, immunofluorescence analyses
Comments, role: minor β-1,3-linked 6-deoxy-Hepp might be existing separately; NMR temperature was not specified.
Related record ID(s): 10446, 10447, 10979
NCBI Taxonomy refs (TaxIDs): 1229785
Show glycosyltransferases
NMR conditions: in D2O
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6 C7
2 Ac ? 19.10
bDSugp 94.95 68.27 77.66 67.71 71.47 32.30 56.87
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6 H7
2 Ac - 2.08
bDSugp 4.88 5.26 3.97 3.43 3.45 1.67-2.12 3.73-3.73
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6 C7/H7
2 Ac 19.10/2.08
bDSugp 94.95/4.88 68.27/5.26 77.66/3.97 67.71/3.43 71.47/3.45 32.30/1.67-2.12 56.87/3.73-3.73
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 | H7 |
|---|
| 2 | Ac |
| 2.08 | |
| | bDSugp | 4.88 | 5.26 | 3.97 | 3.43 | 3.45 | 1.67
2.12 | 3.73
3.73 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 | C7 |
|---|
| 2 | Ac | ? | 19.10 | |
| | bDSugp | 94.95 | 68.27 | 77.66 | 67.71 | 71.47 | 32.30 | 56.87 |
|
![](nmr_drawer.py?width=750&height=130&nucleus=C&rotate_labels=false&spectrum=[{"s":19.1,"l":"2","c":[0,0,0]},{"s":94.95,"l":"1","c":[0,0,225]},{"s":68.27,"l":"2","c":[0,0,225]},{"s":77.66,"l":"3","c":[0,0,225]},{"s":67.71,"l":"4","c":[0,0,225]},{"s":71.47,"l":"5","c":[0,0,225]},{"s":32.3,"l":"6","c":[0,0,225]},{"s":56.87,"l":"7","c":[0,0,225]}])
The spectrum also has 1 signal at unknown position (not plotted). |
There is only one chemically distinct structure:
OC(C)=O](smiles2image.php?&smiles=%2A%5B1%2A%5D%28%2A%29OC%28C%29%3DO&minwidth=240)
R1 = bDSugp, Sug = 6-deoxy-Hepp
*[1*](*)OC(C)=O
59.044 g/mol (C2H3R3O2, R = next and previous repeats / underdetermined substituent(s), not counted in mol weight)
Found 
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