Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11:
XN3LD 
];
infection due to Burkholderia mallei [ICD11:
XN6Y3 ]
NCBI PubMed ID: 34474230Publication DOI: 10.1016/j.jpba.2021.114340Journal NLM ID: 8309336Publisher: London: Elsevier
Correspondence: (J. Yan <zijie1011
yeah.net>
Institutions: Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University), Chongqing 400038, PR China
Burkholderia pseudomallei causes melioidosis - an infectious disease with high mortality. Its varied clinical manifestations and resistance to many antibiotics make it a potential biothreat agent and calls for a robust diagnostic assay and effective vaccines. Bacterial cell surface polysaccharides are considered a valuable target for diagnostics and as protective antigen candidates. This study characterized the structure of polysaccharides of B. pseudomallei clinical strain from Hainan, China. A novel structural domain [→3-(α-D-Manp-1→3-α-D-Manp)2-2Me-α-L-6dTalp-1→] was identified by chemical analysis, gas chromatography-mass spectrometry (GC-MS), and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Immunofluorescence and enzyme-linked immunosorbent assay (ELISA) showed that the serum antibodies against the purified polysaccharide antigen could recognize and bind specifically to B. pseudomallei strains. Additionally, the assays revealed cross-reactivity with polysaccharides from different clinical strains. The polysaccharide antigen also exhibited a strong reaction with the sera from melioidosis patients. Thus, the pentasaccharide repeating unit residue could be a potential candidate antigen for the melioidosis serodiagnosis and vaccine development.
polysaccharide, Burkholderia pseudomallei, Structural characterization, immunological characteristics
Structure type: polymer chemical repeating unit
Location inside paper: p. 114340-9, type A OPS
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, ELISA, anion-exchange chromatography, composition analysis, GPC, UV, extraction, statistical analysis, immunization, immunofluorescence analyses
Related record ID(s): 10445, 10447, 10979
NCBI Taxonomy refs (TaxIDs): 28450,
57975,
13373Reference(s) to other database(s): GTC:G03850AE, GlycomeDB:
3383
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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