Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11:
XN3LD 
]
NCBI PubMed ID: 34474230Publication DOI: 10.1016/j.jpba.2021.114340Journal NLM ID: 8309336Publisher: London: Elsevier
Correspondence: (J. Yan <zijie1011
yeah.net>
Institutions: Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University), Chongqing 400038, PR China
Burkholderia pseudomallei causes melioidosis - an infectious disease with high mortality. Its varied clinical manifestations and resistance to many antibiotics make it a potential biothreat agent and calls for a robust diagnostic assay and effective vaccines. Bacterial cell surface polysaccharides are considered a valuable target for diagnostics and as protective antigen candidates. This study characterized the structure of polysaccharides of B. pseudomallei clinical strain from Hainan, China. A novel structural domain [→3-(α-D-Manp-1→3-α-D-Manp)2-2Me-α-L-6dTalp-1→] was identified by chemical analysis, gas chromatography-mass spectrometry (GC-MS), and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Immunofluorescence and enzyme-linked immunosorbent assay (ELISA) showed that the serum antibodies against the purified polysaccharide antigen could recognize and bind specifically to B. pseudomallei strains. Additionally, the assays revealed cross-reactivity with polysaccharides from different clinical strains. The polysaccharide antigen also exhibited a strong reaction with the sera from melioidosis patients. Thus, the pentasaccharide repeating unit residue could be a potential candidate antigen for the melioidosis serodiagnosis and vaccine development.
polysaccharide, Burkholderia pseudomallei, Structural characterization, immunological characteristics
Structure type: polymer chemical repeating unit
Location inside paper: p. 114340-10
Trivial name: K-antigen, galactan-Kdo, Kdo-EPS
Compound class: CPS, EPS
Contained glycoepitopes: IEDB_115013,IEDB_130645,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149558,IEDB_151528,IEDB_190606,IEDB_918314,SB_165,SB_166,SB_187,SB_195,SB_36,SB_7,SB_87,SB_88,SB_9
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, ELISA, anion-exchange chromatography, composition analysis, GPC, UV, extraction, statistical analysis, immunization, immunofluorescence analyses
Related record ID(s): 10445, 10446, 10979
NCBI Taxonomy refs (TaxIDs): 28450Reference(s) to other database(s): GTC:G77110LE, GlycomeDB:
25312
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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