Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
Publication DOI: 10.1016/j.prostaglandins.2004.09.004Journal NLM ID: 9808648Correspondence: murphyr
njc.org
Institutions: Department of Pharmacology, University of Colorado Health Sciences Center, Campus Box C236, 4200 E. 9th Avenue, Denver, CO 80262, USA
Mass spectrometry has made significant advances in the analysis of lipid substances, both simple and complex present in extracts of eukaryotic and prokaryotic cells. The development of the ionization techniques of electrospray ionization and matrix-assisted laser desorption ionization (MALDI) have both been applied to the analysis of lipids. The example of the types of structural information that can be obtained from MALDI-TOF tandem mass spectrometry is exemplified by the analysis of Kdo2-lipid A, a complex lipopolysaccharide known to activate toll-like 4 receptors on mammalian cells. Analysis of Kdo2-lipid A obtained from an Escherichia coli WBB06 was found to generate an abundant [M-H]- ion at m/z 2236.4 and a more abundant carbon-13 isotope at m/z 2237.4. Furthermore, collisional activation of the lipid A portion of the molecule at m/z 1796.3 resulted in a series of ions corresponding to the loss of all four fatty acyl groups as neutral carboxylic acids. An altogether different challenge of mass spectrometry applied to the area of lipid analysis is that of quantitative analysis. Two rather different requirements have emerged. One with high precision and accuracy for the measurement of relatively few lipid species that are produced at very low concentrations and typically interact with specific receptor proteins. A rather different challenge is that for the analysis of abundant lipid classes, which are composed of multiple molecular species that can approach several hundred under certain circumstances.
mass spectrometry, MALDI-TOF, Lipid analysis, Kdo2–lipid A
Structure type: oligomer
Location inside paper: p. 134
Aglycon: lipid A
Trivial name: outer core region
Compound class: core oligosaccharide, LOS, LPS
Contained glycoepitopes: IEDB_130650,IEDB_130659
Methods: ESI-MS, MALDI-MS
Related record ID(s): 13, 829, 852, 875, 924, 6252, 6306, 6431, 7184, 8403, 10584, 23706, 24940
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G36348JQ, GlycomeDB:
5576
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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