Saksena R, Adamo R, Kovác P Studies toward a conjugate vaccine for anthrax. Synthesis and characterization of anthrose [4,6-dideoxy-4-(3-hydroxy-3-methylbutanamido)-2-O-methyl-D-glucopyranose] and its methyl glycosides Carbohydrate Research340(9) (2005)
1591-1600
NCBI PubMed ID:15922317 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: kpnhelix.nih.gov Institutions: NIDDK, LMC, National Institutes of Health, Bethesda, MD 20892-0815, USA
The key step in the first chemical synthesis of anthrose (16) and its methyl α- (6) and β-glycoside (22) was inversion of configuration at C-2 in triflates 10, 2, and 18, respectively, obtained from the common intermediate, methyl 4-azido-3-O-benzyl-4,6-dideoxy-α-D-mannopyranoside (1). To prepare methyl α-anthroside (6), methylation at O-2 of the gluco product 3, obtained from 2, was followed by hydrogenation/hydrogenolysis of the formed 2-methyl ether 4, to simultaneously remove the protecting benzyl group and reduce the azido function. Subsequent N-acylation of the formed amine 5 with 3-hydroxy-3-methylbutyric acid gave the target methyl α-glycoside 6. Synthesis of methyl β-anthroside (22) comprised the same sequence of reactions, starting from the known methyl 4-azido-3-O-benzyl-4,6-dideoxy-β-D-mannopyranoside (17), which was prepared from 1. In the synthesis of anthrose (16), 1-thio-β-glucoside 11, obtained from 1 through 10, was methylated at O-2, and the azido function in the resulting benzylated 1-thioglycoside 12 was selectively reduced to give amine 13. After N-acylation with 3-hydroxy-3-methylbutyric acid, 1-thioglycoside 14 was hydrolyzed to give the corresponding reducing sugar, aldol 15, which was debenzylated to afford anthrose.
Methods: chemical methods Synthetic data: chemical Comments, role: terminal tetrasaccharide of the surface glycoprotein
Related record ID(s): 10768, 10769, 10812, 22680, 23415, 23501 NCBI Taxonomy refs (TaxIDs):1392 Reference(s) to other database(s): GlycomeDB:27777 Show glycosyltransferases
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