Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
NCBI PubMed ID: 33440288Publication DOI: 10.1016/j.carres.2020.108231Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Paola Cescutti <pcescutti
units.it>
Institutions: Department of Chemistry, University of Cape Town, Rondebosch, 7701, South Africa, Department of Life Sciences, University of Trieste, Via L. Giorgieri 1, Bdg. C11, 34127, Trieste, Italy, Department of Food Science, Cornell University, Ithaca, NY, 14853, USA, Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200, Copenhagen, Denmark
Burkholderia cenocepacia belongs to the Burkholderia Cepacia Complex, a group of 22 closely related species both of clinical and environmental origin, infecting cystic fibrosis patients. B. cenocepacia accounts for the majority of the clinical isolates, comprising the most virulent and transmissible strains. The capacity to form biofilms is among the many virulence determinants of B. cenocepacia, a characteristic that confers enhanced tolerance to some antibiotics, desiccation, oxidizing agents, and host defenses. Exopolysaccharides are a major component of biofilm matrices, particularly providing mechanical stability to biofilms. Recently, a water-insoluble exopolysaccharide produced by B. cenocepacia H111 in biofilm was characterized. In the present study, a water-soluble exopolysaccharide was extracted from B. cenocepacia H111 biofilm, and its structure was determined by GLC-MS, NMR and ESI-MS. The repeating unit is a linear rhamno-tetrasaccharide with 50% replacement of a 3-α-L-Rha with a α-3-L-Man. [2)-α-L-Rhap-(1→3)-α-L-[Rhap or Manp]-(1→3)-α-L-Rhap-(1→2)-α-L-Rhap-(1→]n Molecular modelling was used to obtain information about local structural motifs which could give information about the polysaccharide conformation.
NMR, molecular modelling, polysaccharide structure, Biofilm, Burkholderia cenocepacia H111, L-mannose
Structure type: polymer chemical repeating unit
Location inside paper: p. 108231-2, water-insoluble EPS
Trivial name: water-insoluble EPS, Bep
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_144983,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152206,IEDB_190606,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_7,SB_72
Methods: 13C NMR, 1H NMR, GLC-MS, NMR-2D, partial acid hydrolysis, ESI-MS, GLC, MD simulations, composition analysis
3D data: molecular modeling
Related record ID(s): 7865, 7866
NCBI Taxonomy refs (TaxIDs): 1055524Reference(s) to other database(s): GTC:G54448EJ
Show glycosyltransferases
There is only one chemically distinct structure:
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