Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Clostridium difficile [ICD11:
XN0SE 
]
NCBI PubMed ID: 32789783Publication DOI: 10.1007/s10719-020-09937-9Journal NLM ID: 8603310Publisher: Kluwer Academic Publishers
Correspondence: AD Cox <Andrew.Cox
nrc-cnrc.gc.ca>
Institutions: Vaccine Program, Human Health Therapeutics Portfolio, National Research Council, Ottawa, ON, K1A 0R6, Canada
Two known Clostridiodes (Clostridium) difficile surface antigens, a lipoteichoic acid (LTA) and a polysaccharide (PS-II) were isolated and purified in order to prepare glycoconjugate vaccines to the carrier protein human serum albumin utilising a reductive amination strategy. Mice and rabbits were immunized with a prime and two boost strategy and the resulting sera were examined for their ability to recognise the purified homologous antigens and subsequently killed whole cells of C. difficile strains and other Clostridia species. Immunisation derived antisera from rabbits and mice, recognised all strains of C. difficile vegetative cells examined, with generally similar titers from animals that received the LTA or the PS-II conjugates. Sera raised to the LTA conjugates were able to recognise other Clostridia species C. butyricum, C. bifermentans and C. subterminale whereas sera raised to the PS-II conjugates were not. These LTA and PS-II sera recognised live cells in an immunofluorescence assay and were also able to recognise the spore form of the bacterium. This study has confirmed that the LTA and PS-II polysaccharides are both highly conserved surface polymers of C. difficile that are easily accessible to the immune system and as such may have potential as vaccine antigens or as targets for therapeutics to combat C. difficile infection.
conjugate vaccine, lipoteichoic acid, capsular polysaccharide PS-II, Clostridiodes difficile
Structure type: polymer chemical repeating unit
Location inside paper: p. 496, PS-II
Trivial name: cell-surface polysaccharide, hexaglycosyl phosphate repeating unit cell-surface polysaccharide, PSII
Compound class: CPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_130648,IEDB_130701,IEDB_137473,IEDB_142488,IEDB_144983,IEDB_144996,IEDB_144998,IEDB_146664,IEDB_152206,IEDB_189516,IEDB_241118,IEDB_983930,IEDB_983931,SB_192,SB_44,SB_67,SB_72
Methods: 1H NMR, periodate oxidation, SDS-PAGE, ELISA, Western blotting, MALDI-TOF MS, de-O-acetylation, HPLC, immunization, conjugation, reductive amination, immunofluorescence
Related record ID(s): 7902
NCBI Taxonomy refs (TaxIDs): 272563Reference(s) to other database(s): GTC:G48239WN, GlycomeDB:
36946
Show glycosyltransferases
There is only one chemically distinct structure:
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