Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Bacillus [ICD11:
XN9ZE 
]
The structure was elucidated in this paperNCBI PubMed ID: 34237575Publication DOI: 10.1016/j.foodchem.2021.130496Journal NLM ID: 7702639Publisher: Elsevier Applied Science Publishers
Correspondence: yqtian
scu.edu.cn; pengbiyu
scu.edu.cn
Institutions: College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, PR China, Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, Chengdu 610065, PR China
An exopolysaccharide, EPS-B108, was isolated from the fermented broth (with a yield of 11.3 g/L) of halotolerant Bacillus sp. SCU-E108 by ethanol precipitation, anion-exchange and gel-filtration chromatography, and well characterized by means of physical, chemical and spectral techniques. Data indicated that EPS-B108 was composed solely of fructose with a high molecular weight of 3.578 × 107 g/mol, and contained a β-(2→6)-linked d-Fruf backbone with a single β-d-Fruf at C-1 position. An irregular saccular- or cake-like shape was observed under the enlarged view. It showed no acute oral toxicity in mice, and had good thermal stability (242 °C), solubility in water (91.3%) and oil-holding capacity (1717.0%). Steady-shear flow and dynamical viscoelasticity of aqueous EPS-B108 solutions varied with the polymer concentration, shear rate and temperature, and were described by the Power-law model. Together, these findings support the further application of EPS-B108 as an alternative source of functional food additives and ingredients.
structure, exopolysaccharide, function, Rheology, Bacillus sp., levan
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 3, table S2, EPS-B108
Trivial name: levan
Compound class: CPS, EPS
Contained glycoepitopes: IEDB_923066
Methods: 13C NMR, 1H NMR, methylation, gel filtration, NMR-2D, GC-MS, X-ray, TLC, FTIR, composition analysis, HPSEC-MALLS, UV, HPAEC-PAD, statistical analysis, rheological study, SEM, emulsifying activity determination
NCBI Taxonomy refs (TaxIDs): 1386Reference(s) to other database(s): GTC:G59138IY
Show glycosyltransferases
NMR conditions: in D2O at 298 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6 bDFruf 61.48 105.78 77.87 76.78 81.87 64.94
6,1 bDFruf 61.62 105.08 78.44 75.81 82.59 63.72
6 bDFruf 61.98 105.74 78.28 76.72 81.90 64.88
bDFruf 61.48 105.78 77.87 76.78 81.87 64.94
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6 bDFruf 3.72-3.79 - 4.22 4.13 3.98 3.59-3.93
6,1 bDFruf 3.67-3.78 - 4.25 4.14 3.96 3.76-3.88
6 bDFruf 3.69-3.81 - 4.23 4.12 3.99 3.60-3.94
bDFruf 3.72-3.79 - 4.22 4.13 3.98 3.59-3.93
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6 bDFruf 61.48/3.72-3.79 77.87/4.22 76.78/4.13 81.87/3.98 64.94/3.59-3.93
6,1 bDFruf 61.62/3.67-3.78 78.44/4.25 75.81/4.14 82.59/3.96 63.72/3.76-3.88
6 bDFruf 61.98/3.69-3.81 78.28/4.23 76.72/4.12 81.90/3.99 64.88/3.60-3.94
bDFruf 61.48/3.72-3.79 77.87/4.22 76.78/4.13 81.87/3.98 64.94/3.59-3.93
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6 | bDFruf | 3.72
3.79 |
| 4.22 | 4.13 | 3.98 | 3.59
3.93 |
| 6,1 | bDFruf | 3.67
3.78 |
| 4.25 | 4.14 | 3.96 | 3.76
3.88 |
| 6 | bDFruf | 3.69
3.81 |
| 4.23 | 4.12 | 3.99 | 3.60
3.94 |
| | bDFruf | 3.72
3.79 |
| 4.22 | 4.13 | 3.98 | 3.59
3.93 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6 | bDFruf | 61.48 | 105.78 | 77.87 | 76.78 | 81.87 | 64.94 |
| 6,1 | bDFruf | 61.62 | 105.08 | 78.44 | 75.81 | 82.59 | 63.72 |
| 6 | bDFruf | 61.98 | 105.74 | 78.28 | 76.72 | 81.90 | 64.88 |
| | bDFruf | 61.48 | 105.78 | 77.87 | 76.78 | 81.87 | 64.94 |
|
There is only one chemically distinct structure:
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Found 1 record.
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