Kaszowska M, Gorska S, Knirel Y, Kalinchuk N, Gamian A, Katzenellenbogen E Structural analysis of Edwardsiella tarda PCM 1155 O-polysaccharide revealed the presence of unique β-L-RhapNAc3NAc derivative Carbohydrate Research509 (2021)
108423
The structure was elucidated in this paper NCBI PubMed ID:34507179 Publication DOI:10.1016/j.carres.2021.108423 Journal NLM ID:0043535 Publisher: Elsevier Correspondence: S. Gorska <sabina.gorskahirszfeld.pl> Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, R. Weigla 12, PL-53-114, Wroclaw, Poland
The chemical structure of the lipopolysaccharide O-polysaccharide repeating unit of Edwardsiella tarda strain PCM 1155 was studied for the first time. The complete structure of repeating unit was investigated by chemical methods, 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The rarely occurring monosaccharide, 2,3-diacetamido-2,3,6-trideoxy-l-mannose (L-RhapNAc3NAc) was identified. The following structure was established.
Kaszowska M, de Mendoza-Barbero E, Maciejewska A, Merino S, Lugowski C, Tomas JM The Complete Structure of the Core Oligosaccharide from Edwardsiella tarda EIB 202 Lipopolysaccharide International Journal of Molecular Sciences18(6) (2017)
1163
The structure was elucidated in this paper NCBI PubMed ID:28561753 Publication DOI:10.3390/ijms18061163 Journal NLM ID:101092791 Publisher: Basel, Switzerland: MDPI Correspondence: Marta Kaszowska <marta.kaszowskaiitd.pan.wroc.pl> Institutions: Department of Biotechnology and Molecular Biology, University of Opole, PL-Opole, Poland, Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Department of Microbiology, University of Barcelona, Diagonal 643, 08071 Barcelona, Spain
The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry.
NMR, core oligosaccharide, MALDI-TOF MS, Edwardsiella tarda, ESI MSn, genomic
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, 31P NMR, ESI-MS, mild acid hydrolysis, MALDI-TOF MS, function analysis of gene clusters Comments, role: the NMR solution was not indicated; The positions of glycine in OSVIII and OSVII were not determined. In OS glycoform terminal α-D-GlcpN residue was partial replacement by α-D-GlcpNAc residue.
Kaszowska M, de Mendoza-Barbero E, Maciejewska A, Merino S, Lugowski C, Tomas JM The Complete Structure of the Core Oligosaccharide from Edwardsiella tarda EIB 202 Lipopolysaccharide International Journal of Molecular Sciences18(6) (2017)
1163
The structure was elucidated in this paper NCBI PubMed ID:28561753 Publication DOI:10.3390/ijms18061163 Journal NLM ID:101092791 Publisher: Basel, Switzerland: MDPI Correspondence: Marta Kaszowska <marta.kaszowskaiitd.pan.wroc.pl> Institutions: Department of Biotechnology and Molecular Biology, University of Opole, PL-Opole, Poland, Department of Immunochemistry, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland, Department of Microbiology, University of Barcelona, Diagonal 643, 08071 Barcelona, Spain
The chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharide of pathogenic Edwardsiella tarda strain EIB 202 were studied for the first time. The complete gene assignment for all LPS core biosynthesis gene functions was acquired. The complete structure of core oligosaccharide was investigated by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry MSn, and matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry. The following structure of the undecasaccharide was established: The heterogeneous appearance of the core oligosaccharide structure was due to the partial lack of β-d-Galp and the replacement of α-d-GlcpNAcGly by α-d-GlcpNGly. The glycine location was identified by mass spectrometry.
NMR, core oligosaccharide, MALDI-TOF MS, Edwardsiella tarda, ESI MSn, genomic
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, sugar analysis, 31P NMR, ESI-MS, mild acid hydrolysis, MALDI-TOF MS, function analysis of gene clusters Comments, role: the NMR solution was not indicated; The positions of glycine in OSVIII and OSVII were not determined.
Jordán M, Wojtys-Tekiel S, Merino S, Tomas JM, Kaszowska M Structural Diversity among Edwardsiellaceae Core Oligosaccharides International Journal of Molecular Sciences24(5) (2023)
4768
The structure was elucidated in this paper NCBI PubMed ID:36902212 Publication DOI:10.3390/ijms24054768 Journal NLM ID:101092791 Publisher: Basel, Switzerland: MDPI Correspondence: S. Merino <smerinoub.edu>; M. Kaszowska <marta.kaszowskahirszfeld.pl> Institutions: Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland, Department of Genetic, Microbiology and Statistic, University of Barcelona, Diagonal 643, 08028 Barcelona, Spain
The Edwardsiella genus presents five different pathogenic species: Edwardsiella tarda, E. anguillarum, E. piscicida, E. hoshinae and E. ictaluri. These species cause infections mainly in fish, but they can also infect reptiles, birds or humans. Lipopolysaccharide (endotoxin) plays an important role in the pathogenesis of these bacteria. For the first time, the chemical structure and genomics of the lipopolysaccharide (LPS) core oligosaccharides of E. piscicida, E. anguillarum, E. hoshinae and E. ictaluri were studied. The complete gene assignments for all core biosynthesis gene functions were acquired. The structure of core oligosaccharides was investigated by ¹H and 13C nuclear magnetic resonance (NMR) spectroscopy. The structures of E. piscicida and E. anguillarum core oligosaccharides show the presence of →3,4)-L-glycero-α-D-manno-Hepp, two terminal β-D-Glcp, →2,3,7)-L-glycero-α-D-manno-Hepp, →7)-L-glycero-α-D-manno-Hepp, terminal α-D-GlcpN, two →4)-α-D-GalpA, →3)-α-D-GlcpNAc, terminal β-D-Galp and →5-substituted Kdo. E. hoshinare core oligosaccharide shows only one terminal β-D-Glcp, and instead of terminal β-D-Galp a terminal α-D-GlcpNAc. E. ictaluri core oligosaccharide shows only one terminal β-D-Glcp, one →4)-α-D-GalpA and do not have terminal α-D-GlcpN (see complementary figure)
report error
Found 4 records.
Displayed records from 1 to 4
hirszfeld.pl>
]; meningitis [ICD11: 1D01