Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 34757131Publication DOI: 10.1016/j.ijbiomac.2021.10.178Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: asyashpirt
gmail.com
Institutions: Central Scientific Research Institute of Epidemiology, Moscow, Russia, Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology. Brisbane, Australia, D.I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Science, Moscow, Russia, M.M. Shemyakin and Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow, Region, Russia
Whole genome sequence from Acinetobacter baumannii isolate Ab-46-1632 reveals a novel KL144 capsular polysaccharide (CPS) biosynthesis gene cluster, which carries genes for d-glucuronic acid (D-GlcA) and l-rhamnose (l-Rha) synthesis. The CPS was extracted from Ab-46-1632 and studied by 1H and 13C NMR spectroscopy, including a two-dimensional 1H,13C HMBC experiment and Smith degradation. The CPS was found to have a hexasaccharide repeat unit composed of four l-Rhap residues and one residue each of d-GlcpA and N-acetyl-d-glucosamine (D-GlcpNAc) consistent with sugar synthesis genes present in KL144. The K144 CPS structure was established and found to be related to those of A. baumannii K55, K74, K85, and K86. A comparison of the corresponding gene clusters to KL144 revealed a number of shared glycosyltransferase genes correlating to shared glycosidic linkages in the structures. One from the enzymes, encoded by only KL144 and KL86, is proposed to be a novel multifunctional rhamnosyltransfaerase likely responsible for synthesis of a shared α-l-Rhap-(1→2)-α-L-Rhap-(1→3)-L-Rhap trisaccharide fragment in the K144 and K86 structures.
Acinetobacter baumannii, capsular polysaccharide, K locus, K144, K86, multifunctional glycosyltransferase
Structure type: polymer chemical repeating unit
Location inside paper: p. 1297, Fig. 2, K144
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_125613,IEDB_133754,IEDB_135813,IEDB_135849,IEDB_136105,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_141815,IEDB_151531,IEDB_225177,IEDB_423153,IEDB_885823
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, acid hydrolysis, GLC, Smith degradation, GPC, bioinformatic analysis, HR-ESI-MS, sequencing
Enzymes that release or process the structure: Grt78, Grt221, Grt81, Grt81, Grt82, Wzy(K144)[ItrA3]
Related record ID(s): 5869, 5870, 5871, 5872, 5873, 5874
NCBI Taxonomy refs (TaxIDs): 470
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,2,3,4 aLRhap 101.9 71.2 81.6 72.3 70.5 17.7
3,3,2,3 bDGlcpA 104.9 74.9 75.4 80.3 76.1 175.1
3,3,2 aLRhap 103.2 71.0 81.7 72.4 70.5 18.0
3,3 aLRhap 102.1 79.2 71.4 73.5 70.5 17.8
3 aLRhap 102.5 71.9 78.7 73.0 70.5 17.7
2 Ac 176.1 23.8
bDGlcpN 103.6 56.9 82.9 69.7 77.1 62.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,2,3,4 aLRhap 4.75 4.15 3.78 3.49 4.02 1.23
3,3,2,3 bDGlcpA 4.72 3.44 3.61 3.64 3.93 -
3,3,2 aLRhap 4.96 4.28 3.91 3.60 3.75 1.27
3,3 aLRhap 5.15 4.05 3.91 3.47 3.75 1.31
3 aLRhap 4.85 3.85 3.79 3.54 4.02 1.23
2 Ac - 2.05
bDGlcpN 4.71 3.86 3.63 3.56 3.47 3.77-3.92
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,2,3,4 aLRhap 101.9/4.75 71.2/4.15 81.6/3.78 72.3/3.49 70.5/4.02 17.7/1.23
3,3,2,3 bDGlcpA 104.9/4.72 74.9/3.44 75.4/3.61 80.3/3.64 76.1/3.93
3,3,2 aLRhap 103.2/4.96 71.0/4.28 81.7/3.91 72.4/3.60 70.5/3.75 18.0/1.27
3,3 aLRhap 102.1/5.15 79.2/4.05 71.4/3.91 73.5/3.47 70.5/3.75 17.8/1.31
3 aLRhap 102.5/4.85 71.9/3.85 78.7/3.79 73.0/3.54 70.5/4.02 17.7/1.23
2 Ac 23.8/2.05
bDGlcpN 103.6/4.71 56.9/3.86 82.9/3.63 69.7/3.56 77.1/3.47 62.0/3.77-3.92
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,2,3,4 | aLRhap | 4.75 | 4.15 | 3.78 | 3.49 | 4.02 | 1.23 |
| 3,3,2,3 | bDGlcpA | 4.72 | 3.44 | 3.61 | 3.64 | 3.93 |
|
| 3,3,2 | aLRhap | 4.96 | 4.28 | 3.91 | 3.60 | 3.75 | 1.27 |
| 3,3 | aLRhap | 5.15 | 4.05 | 3.91 | 3.47 | 3.75 | 1.31 |
| 3 | aLRhap | 4.85 | 3.85 | 3.79 | 3.54 | 4.02 | 1.23 |
| 2 | Ac |
| 2.05 | |
| | bDGlcpN | 4.71 | 3.86 | 3.63 | 3.56 | 3.47 | 3.77
3.92 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,2,3,4 | aLRhap | 101.9 | 71.2 | 81.6 | 72.3 | 70.5 | 17.7 |
| 3,3,2,3 | bDGlcpA | 104.9 | 74.9 | 75.4 | 80.3 | 76.1 | 175.1 |
| 3,3,2 | aLRhap | 103.2 | 71.0 | 81.7 | 72.4 | 70.5 | 18.0 |
| 3,3 | aLRhap | 102.1 | 79.2 | 71.4 | 73.5 | 70.5 | 17.8 |
| 3 | aLRhap | 102.5 | 71.9 | 78.7 | 73.0 | 70.5 | 17.7 |
| 2 | Ac | 176.1 | 23.8 | |
| | bDGlcpN | 103.6 | 56.9 | 82.9 | 69.7 | 77.1 | 62.0 |
|
There is only one chemically distinct structure:
Found 
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