Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
NCBI PubMed ID: 34604306Publication DOI: 10.3389/fmolb.2021.727980Journal NLM ID: 101653173Publisher: Lausanne: Frontiers Media S.A.
Correspondence: Jesus Angulo <j.angulo
uea.ac.uk; jangulo
us.es>
Institutions: School of Pharmacy, University of East Anglia, Norwich, United Kingdom, Department Life Sciences, University of Trieste, Trieste, Italy, Department of Organic Chemistry, Faculty of Chemistry, University of Seville, Seville, Spain, Instituto de Investigaciones Quimicas (CSIC-US), Seville, Spain
Biofilms confine bacterial cells within self-produced matrices, offering advantages such as protection from antibiotics and entrapment of nutrients. Polysaccharides are major components in these macromolecular assemblies, and their interactions with other chemicals are of high relevance for the benefits provided by the biofilm 3D molecular matrix. NMR is a powerful technique for the study and characterization of the interactions between molecules of biological relevance. In this study, we have applied multifrequency saturation transfer difference (STD) NMR and DOSY NMR approaches to elucidate the interactions between the exopolysaccharide produced by Burkholderia multivorans C1576 (EpolC1576) and the antibiotics kanamycin and ceftadizime. The NMR strategies presented here allowed for an extensive characterization at an atomic level of the mechanisms behind the implication of the EpolC1576 in the recalcitrance phenomena, which is the ability of bacteria in biofilms to survive in the presence of antibiotics. Our results suggest an active role for EpolC1576 in the recalcitrance mechanisms toward kanamycin and ceftadizime, though through two different mechanisms.
exopolysaccharides, biofilms, Burkholderia multivorans, STD NMR, multifrequency STD NMR
Structure type: polymer chemical repeating unit
Location inside paper: scheme 1, EpolC1576
Trivial name: rhamnomannan exopolysaccharide (EpolC1576), a rhamno-mannan Epol
Compound class: EPS
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_1394181,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: DOSY, 1H STD NMR
NCBI Taxonomy refs (TaxIDs): 87883Reference(s) to other database(s): GTC:G13263FN
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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