Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 34884549Publication DOI: 10.3390/ijms222312746Journal NLM ID: 101092791Publisher: Basel, Switzerland: MDPI
Correspondence: Yujuan Xu <hujing
jiangnan.edu.cn>; Xi Guo <guoxi
nankai.edu.cn>
Institutions: EDA Institute of Biological Sciences and Biotechnology, Nankai University, 23 Hongda Street, Tianjin, China, Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Lihu Ave. 1800, Wuxi, China, Wuxi School of Medicine, Jiangnan University, Lihu Ave. 1800, Wuxi, China
The O-antigen is the outermost component of the lipopolysaccharide layer in Gram-negative bacteria, and the variation of O-antigen structure provides the basis for bacterial serological diversity. Here, we determined the O-antigen structure of an Escherichia coli strain, LL004, which is totally different from all of the E. coli serogroups. The tetrasaccharide repeating unit was determined as →4)-β-d-Galp-(1→3)-β-d-GlcpNAc6OAc(~70%)-(1→3)-β-d-GalpA-(1→3)-β-d-GalpNAc-(1→ with monosaccharide analysis and NMR spectra. We also characterized the O-antigen gene cluster of LL004, and sequence analysis showed that it correlated well with the O-antigen structure. Deletion and complementation testing further confirmed its role in O-antigen biosynthesis, and indicated that the O-antigen of LL004 is assembled via the Wzx/Wzy dependent pathway. Our findings, in combination, suggest that LL004 should represent a novel serogroup of E. coli.
structure, O-antigen, Escherichia coli, serogroup, O-antigen gene cluster
Structure type: polymer chemical repeating unit ; 26100
Location inside paper: abstract, Fig. 5, table 1
Compound class: O-antigen
Contained glycoepitopes: IEDB_130648,IEDB_135813,IEDB_136044,IEDB_137340,IEDB_137472,IEDB_137473,IEDB_1391962,IEDB_141794,IEDB_141807,IEDB_142078,IEDB_143794,IEDB_150899,IEDB_151531,IEDB_190606,SB_137,SB_165,SB_166,SB_187,SB_195,SB_25,SB_29,SB_7,SB_88
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, sugar analysis, de-O-acetylation, HPSEC, HPAEC-PAD, function analysis of gene clusters, genome sequencing, annotation
Related record ID(s): 10392
NCBI Taxonomy refs (TaxIDs): 562
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,3,3 bDGalp 103.0-103.3 71.1 72.3 76.3 74.7 60.8
3,3,2 Ac 174.9 22.4
3,3,6 70%Ac 174.0 20.3
3,3 bDGlcpN 102.6 55.1 79.0 72.5 72.1 62.8
3 bDGalpA 102.7 69.1 80.4 69.6 76.9 175.0
2 Ac 174.9 22.4
bDGalpN 102.6 51.4 79.6 68.1 72.3 60.8
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,3,3 bDGalp 4.30-4.38 3.31 3.67 4.02 3.62 3.69
3,3,2 Ac - 1.94
3,3,6 70%Ac - 2.07
3,3 bDGlcpN 4.61 3.72 3.64 3.65 3.73 4.31-4.44
3 bDGalpA 4.71 3.55 3.52 4.47 4.27 -
2 Ac - 1.94
bDGalpN 4.60 3.95 3.80 4.12 3.63 3.69
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,3,3 bDGalp 103.0-103.3/4.30-4.38 71.1/3.31 72.3/3.67 76.3/4.02 74.7/3.62 60.8/3.69
3,3,2 Ac 22.4/1.94
3,3,6 70%Ac 20.3/2.07
3,3 bDGlcpN 102.6/4.61 55.1/3.72 79.0/3.64 72.5/3.65 72.1/3.73 62.8/4.31-4.44
3 bDGalpA 102.7/4.71 69.1/3.55 80.4/3.52 69.6/4.47 76.9/4.27
2 Ac 22.4/1.94
bDGalpN 102.6/4.60 51.4/3.95 79.6/3.80 68.1/4.12 72.3/3.63 60.8/3.69
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,3,3 | bDGalp | 4.30
4.38 | 3.31 | 3.67 | 4.02 | 3.62 | 3.69 |
| 3,3,2 | Ac |
| 1.94 | |
| 3,3,6 | 70%Ac |
| 2.07 | |
| 3,3 | bDGlcpN | 4.61 | 3.72 | 3.64 | 3.65 | 3.73 | 4.31
4.44 |
| 3 | bDGalpA | 4.71 | 3.55 | 3.52 | 4.47 | 4.27 |
|
| 2 | Ac |
| 1.94 | |
| | bDGalpN | 4.60 | 3.95 | 3.80 | 4.12 | 3.63 | 3.69 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,3,3 | bDGalp | 103.0
103.3 | 71.1 | 72.3 | 76.3 | 74.7 | 60.8 |
| 3,3,2 | Ac | 174.9 | 22.4 | |
| 3,3,6 | 70%Ac | 174.0 | 20.3 | |
| 3,3 | bDGlcpN | 102.6 | 55.1 | 79.0 | 72.5 | 72.1 | 62.8 |
| 3 | bDGalpA | 102.7 | 69.1 | 80.4 | 69.6 | 76.9 | 175.0 |
| 2 | Ac | 174.9 | 22.4 | |
| | bDGalpN | 102.6 | 51.4 | 79.6 | 68.1 | 72.3 | 60.8 |
|
There is only one chemically distinct structure:
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Found 1 record.
Displayed record 1
