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Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Burkholderia pseudomallei [ICD11: XN3LD

]

The structure was elucidated in this paper
NCBI PubMed ID: 34474230
Publication DOI: 10.1016/j.jpba.2021.114340
Journal NLM ID: 8309336
Publisher: London: Elsevier
Correspondence: (J. Yan <zijie1011

yeah.net>
Institutions: Department of Clinical Microbiology and Immunology, College of Pharmacy and Medical Laboratory, Army Medical University (Third Military Medical University), Chongqing 400038, PR China

Burkholderia pseudomallei causes melioidosis - an infectious disease with high mortality. Its varied clinical manifestations and resistance to many antibiotics make it a potential biothreat agent and calls for a robust diagnostic assay and effective vaccines. Bacterial cell surface polysaccharides are considered a valuable target for diagnostics and as protective antigen candidates. This study characterized the structure of polysaccharides of B. pseudomallei clinical strain from Hainan, China. A novel structural domain [→3-(α-D-Manp-1→3-α-D-Manp)2-2Me-α-L-6dTalp-1→] was identified by chemical analysis, gas chromatography-mass spectrometry (GC-MS), and 1D/2D nuclear magnetic resonance (NMR) spectroscopy. Immunofluorescence and enzyme-linked immunosorbent assay (ELISA) showed that the serum antibodies against the purified polysaccharide antigen could recognize and bind specifically to B. pseudomallei strains. Additionally, the assays revealed cross-reactivity with polysaccharides from different clinical strains. The polysaccharide antigen also exhibited a strong reaction with the sera from melioidosis patients. Thus, the pentasaccharide repeating unit residue could be a potential candidate antigen for the melioidosis serodiagnosis and vaccine development.

polysaccharide, Burkholderia pseudomallei, Structural characterization, immunological characteristics

Structure type: structural motif or average structure
Location inside paper: abstract, p. 114340-6, table 3, BPC006-BPPI-b1 fraction
Trivial name: antigen epitope
Compound class: surface polysaccharide
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_140116,IEDB_144983,IEDB_152206,IEDB_164174,IEDB_76933,IEDB_983930,SB_197,SB_44,SB_67,SB_72

Methods: 13C NMR, 1H NMR, methylation, NMR-2D, GC-MS, ELISA, anion-exchange chromatography, composition analysis, GPC, UV, extraction, statistical analysis, immunization, immunofluorescence analyses
Comments, role: NMR temperature was not specified. Possible error in the NMR assignment (Table 1): the same subspectrum is attributed to multiple aDManp residues in different structural surrounding.

Related record ID(s): 10445, 10446, 10447
NCBI Taxonomy refs (TaxIDs): 1229785
Show glycosyltransferases

NMR conditions: in D2O [as TSV]

13C NMR data:
Linkage	Residue	C1	C2	C3	C4	C5	C6
3	aDManp
2	Me	55.46
	aL6dTalp	100.39	76.69	72.92	68.44	70.48	14.29


1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
3	aDManp
2	Me	3.35
	aL6dTalp	5.23	3.68	4.08	4.09	4.30	1.18


1H/13C HSQC data:
Linkage	Residue	C1/H1	C2/H2	C3/H3	C4/H4	C5/H5	C6/H6
3	aDManp
2	Me	55.46/3.35
	aL6dTalp	100.39/5.23	76.69/3.68	72.92/4.08	68.44/4.09	70.48/4.30	14.29/1.18

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
3	aDManp
2	Me	3.35
	aL6dTalp	5.23	3.68	4.08	4.09	4.30	1.18

¹³C NMR data:

Linkage	Residue	C1	C2	C3	C4	C5	C6
3	aDManp
2	Me	55.46
	aL6dTalp	100.39	76.69	72.92	68.44	70.48	14.29

There is only one chemically distinct structure:

*O[C@H]1[C@H](O)[C@@H](CO)O[C@H](O[C@@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](O)[C@@H](O[C@@H]4[C@H](O)[C@@H](O[C@H]5[C@@H](OC)[C@H](*)O[C@@H](C)[C@H]5O)O[C@H](CO)[C@H]4O)O[C@H](CO)[C@H]3O)O[C@H](CO)[C@H]2O)[C@H]1O

SVG MW SMILES 3D mol Ionize