Taxonomic group: fungi / Ascomycota
(Phylum: Ascomycota)
NCBI PubMed ID: 8287049Journal NLM ID: 9311984Publisher: Pharmaceutical Society of Japan
Institutions: Faculty of Pharmaceutical Sciences, Kinki University, Higashi-Osaka, Japan
Four isolectins were isolated from bulbs of Croccus sativum (saffron), using affinity chromatography on mannan-Sepharose 4B, gel filtration and ion-exchange column chromatography in the presence of 8 M urea. The relative molecular masses of these lectins were determined by gel filtration to be approximately 48 kDa. On polyacrylamide gel electrophoresis, relative molecular masses of 8 kDa were obtained, suggesting that the lectins are hexamers. No carbohydrates were detected in the lectins. The lectins agglutinated the yeasts of Saccharomyces cerevisiae at a minimum concentration of 30 micrograms/ml, however, they did not agglutinate animal erythrocytes (human, sheep, rabbit and mouse) even at a concentration of 1000 micrograms/ml. In inhibitory experiments, mannan from Saccharomyces cerevisiae was the most potent inhibitor of C. sativum lectins. While D-mannose showed no inhibition, manno-oligosaccharides with either (α 1-2, 1-3) or (α 1-6) linkages were potent inhibitors, suggesting that C. sativum lectins recognized manno-oligosaccharide units larger than mannobiose. Ovomucoid also exhibited potent inhibition, however, the other carbohydrates examined did not.
Structure type: homopolymer ; n=2-3
Contained glycoepitopes: IEDB_130701,IEDB_140116,IEDB_141793,IEDB_141828,IEDB_144983,IEDB_152206,IEDB_153220,IEDB_153762,IEDB_153763,IEDB_76933,IEDB_983930,SB_198,SB_44,SB_67,SB_72
Related record ID(s): 43302, 110093, 110095
NCBI Taxonomy refs (TaxIDs): 4932Reference(s) to other database(s): GTC:G55317BB, CCSD:
33993, CBank-STR:4655
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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