Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Campylobacter jejuni [ICD11:
XN4Q5 
]
NCBI PubMed ID: 26676578Publication DOI: 10.1073/pnas.1421082112Journal NLM ID: 7505876Publisher: National Academy of Sciences
Correspondence: c.day
griffith.edu.au; m.jennings
griffith.edu.au
Institutions: Institute for Glycomics, Griffith University Gold Coast Campus, Gold Coast, QLD 4222, Australia, School of Biological Sciences, Department of Molecular and Cellular Biology, University of Adelaide, Adelaide, SA 5005, Australia, Department of Microbiology, University of Iowa, Iowa City, IA 52242
Cells from all domains of life express glycan structures attached to lipids and proteins on their surface, called glycoconjugates. Cell-to-cell contact mediated by glycan:glycan interactions have been considered to be low-affinity interactions that precede high-affinity protein-glycan or protein-protein interactions. In several pathogenic bacteria, truncation of surface glycans, lipooligosaccharide (LOS), or lipopolysaccharide (LPS) have been reported to significantly reduce bacterial adherence to host cells. Here, we show that the saccharide component of LOS/LPS have direct, high-affinity interactions with host glycans. Glycan microarrays reveal that LOS/LPS of four distinct bacterial pathogens bind to numerous host glycan structures. Surface plasmon resonance was used to determine the affinity of these interactions and revealed 66 high-affinity host-glycan:bacterial-glycan pairs with equilibrium dissociation constants (KD) ranging between 100 nM and 50 microM. These glycan:glycan affinity values are similar to those reported for lectins or antibodies with glycans. Cell assays demonstrated that glycan:glycan interaction-mediated bacterial adherence could be competitively inhibited by either host cell or bacterial glycans. This is the first report to our knowledge of high affinity glycan:glycan interactions between bacterial pathogens and the host. The discovery of large numbers of glycan:glycan interactions between a diverse range of structures suggests that these interactions may be important in all biological systems.
Lipopolysaccharide, Lipooligosaccharide, adherence, glycoconjugates, glycan, Glycomics
Structure type: oligomer
Location inside paper: p.E7267, fig.1, C. jejuni 11168-O
The structure in this paper was incorrect:
Aglycon: lipid A
Compound class: LOS
Contained glycoepitopes: IEDB_130648,IEDB_130650,IEDB_134627,IEDB_136044,IEDB_136794,IEDB_137472,IEDB_137473,IEDB_140088,IEDB_141794,IEDB_146100,IEDB_147450,IEDB_147451,IEDB_149174,IEDB_150933,IEDB_190606,IEDB_2189047,SB_116,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_195,SB_23,SB_24,SB_25,SB_39,SB_68,SB_7,SB_8,SB_84,SB_88,SB_96
Methods: de-O-acylation, serological methods, ITC, SPR, conjugation, competition ssays
Biological activity: analysis of glycan:glycan interactions of LOS/LOS
Comments, role: in the paper the structure is given with an error; the structure from Ref [59}.
Related record ID(s): 11138, 11139, 11140, 11141, 11142, 11143, 11144, 11145, 11146, 11147
NCBI Taxonomy refs (TaxIDs): 197Reference(s) to other database(s): GTC:G10779KL
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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