Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Acinetobacter baumannii [ICD11:
XN8LS 
]
The structure was elucidated in this paperNCBI PubMed ID: 31421354Publication DOI: 10.1016/j.carres.2019.107774Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: J.J. Kenyon <johanna.kenyon
qut.edu.au>
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Health and Biomedical Innovation, School of Biomedical Sciences, Queensland University of Technology, Brisbane, Australia, Higher Chemical College of the Russian Academy of Sciences, D. I. Mendeleev University of Chemical Technology of Russia, Moscow, Russia, Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russiag State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russiah Central Scientific Research Institute of Epidemiology, Moscow, Russia
The genome of Acinetobacter baumannii clinical isolate, MAR-303, recovered in Russia was sequenced and found to contain a novel gene cluster at the A. baumannii K locus for capsule biosynthesis. The gene cluster, designated KL116, included four genes for glycosyltransferases (Gtrs) and a gene for a Wzy polymerase responsible for joining oligosaccharide K units into the capsular polysaccharide (CPS). The arrangement of KL116 was a hybrid of previously described A. baumannii gene clusters, with two gtr genes and the wzy gene shared by KL37 and the two other gtr genes found in KL14. The structure of the K116 CPS was established by sugar analysis and Smith degradation, along with one- and two-dimensional 1H and 13C NMR spectroscopy. The CPS is composed of branched pentasaccharide K units containing only neutral sugars, with three monosaccharides in the main chain and a disaccharide side chain. The K116 unit shares internal sugar linkages with the K14 and K37 units, corresponding to the presence of shared gtr genes in the gene clusters. However, the specific linkage formed by Wzy was discrepant between K116 and the previously reported K37 CPS produced by A. baumannii isolate NIPH146. The K37 structure was therefore revised in this study, and the corrected Wzy linkage found to be identical to the Wzy linkage in K116. The KL116, KL14 and KL37 gene clusters were found in genomes of a variety of A. baumannii strain backgrounds, indicating their global distribution.
Acinetobacter baumannii, capsular polysaccharide, K locus, K116, K14, Revised K37
Structure type: polymer chemical repeating unit
Location inside paper: fig.3A, K116
Compound class: CPS
Contained glycoepitopes: IEDB_130648,IEDB_134624,IEDB_134627,IEDB_136044,IEDB_136906,IEDB_137472,IEDB_137473,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_147450,IEDB_151528,IEDB_190606,IEDB_742248,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_21,SB_23,SB_24,SB_25,SB_7,SB_8,SB_88
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, sugar analysis, Smith degradation, ion-exchange chromatography, bioinformatic analysis, sequencing
Enzymes that release or process the structure: Gtr75,Gtr76,Gtr25, Gtr5, (glycosyltransferases) Wzy(K116)[ItrA2] (polymerase)
Related record ID(s): 2291, 2292
NCBI Taxonomy refs (TaxIDs): 470Reference(s) to other database(s): GTC:G13228GE
Show glycosyltransferases
NMR conditions: in D2O at 333 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,6,4,6 bDGlcp 104.1 74.4 77.0 71.1 77.2 62.1
3,6,4,2 Ac 175.7-176.3 23.7-24.1
3,6,4 bDGalpN 103.1 53.6 72.1 69.2 74.8 70.4
3,6 aDGalp 100.4 68.7 80.9 77.6 71.9 62.4
3 bDGalp 106.0 72.0 73.8 69.7 73.6 67.7
2 Ac 175.7-176.3 23.7-24.1
bDGalpN 104.5 52.9 81.6 69.4 76.0 62.7
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,6,4,6 bDGlcp 4.51 3.30 3.50 3.40 3.49 3.75-3.93
3,6,4,2 Ac - 2.03-2.07
3,6,4 bDGalpN 4.92 3.93 3.78 3.97 3.85 3.92-4.08
3,6 aDGalp 4.96 3.74 3.91 4.37 3.94 3.74-3.81
3 bDGalp 4.48 3.56 3.63 4.02 3.84 3.83-3.83
2 Ac - 2.03-2.07
bDGalpN 4.66 4.11 3.85 4.16 3.70 3.78-3.78
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,6,4,6 bDGlcp 104.1/4.51 74.4/3.30 77.0/3.50 71.1/3.40 77.2/3.49 62.1/3.75-3.93
3,6,4,2 Ac 23.7-24.1/2.03-2.07
3,6,4 bDGalpN 103.1/4.92 53.6/3.93 72.1/3.78 69.2/3.97 74.8/3.85 70.4/3.92-4.08
3,6 aDGalp 100.4/4.96 68.7/3.74 80.9/3.91 77.6/4.37 71.9/3.94 62.4/3.74-3.81
3 bDGalp 106.0/4.48 72.0/3.56 73.8/3.63 69.7/4.02 73.6/3.84 67.7/3.83-3.83
2 Ac 23.7-24.1/2.03-2.07
bDGalpN 104.5/4.66 52.9/4.11 81.6/3.85 69.4/4.16 76.0/3.70 62.7/3.78-3.78
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,6,4,6 | bDGlcp | 4.51 | 3.30 | 3.50 | 3.40 | 3.49 | 3.75
3.93 |
| 3,6,4,2 | Ac |
| 2.03
2.07 | |
| 3,6,4 | bDGalpN | 4.92 | 3.93 | 3.78 | 3.97 | 3.85 | 3.92
4.08 |
| 3,6 | aDGalp | 4.96 | 3.74 | 3.91 | 4.37 | 3.94 | 3.74
3.81 |
| 3 | bDGalp | 4.48 | 3.56 | 3.63 | 4.02 | 3.84 | 3.83
3.83 |
| 2 | Ac |
| 2.03
2.07 | |
| | bDGalpN | 4.66 | 4.11 | 3.85 | 4.16 | 3.70 | 3.78
3.78 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,6,4,6 | bDGlcp | 104.1 | 74.4 | 77.0 | 71.1 | 77.2 | 62.1 |
| 3,6,4,2 | Ac | 175.7
176.3 | 23.7
24.1 | |
| 3,6,4 | bDGalpN | 103.1 | 53.6 | 72.1 | 69.2 | 74.8 | 70.4 |
| 3,6 | aDGalp | 100.4 | 68.7 | 80.9 | 77.6 | 71.9 | 62.4 |
| 3 | bDGalp | 106.0 | 72.0 | 73.8 | 69.7 | 73.6 | 67.7 |
| 2 | Ac | 175.7
176.3 | 23.7
24.1 | |
| | bDGalpN | 104.5 | 52.9 | 81.6 | 69.4 | 76.0 | 62.7 |
|
There is only one chemically distinct structure:
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