Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 25839829Publication DOI: 10.1016/j.carbpol.2015.02.038Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: F. Lelchat <florian.lelchat
gmail.com>
Institutions: Laboratoire BMM, centre Ifremer de Brest, ZI pointe du diable, 29280 Plouzané, France, Service commun de résonnance magnétique nucléaire, Faculté de science de Brest, Université de Bretagne Occidentale, 6 av. Victor Le Gorgeu, 29238 Brest Cedex 3, France, Sorbonne Universités, UPMC Paris 06, Station Biologique de Roscoff, UMR 7144, Place George Tessier, 29680 Roscoff, France, CNRS, station biologique de Roscoff, UMR 7144, Place George Tessier, 29680 Roscoff, France, Laboratory of Marine Biotechnology and Microbiology, Graduate School of Fisheries Sciences, Hokkaido University, Minato-cho 3-1-1, Hakodate 041-8611, Japan, Service commun de chromatographie, CERMAV-CNRS, 601 rue de la chimie, St Martin d'Here, 38041 Grenoble, France
We have studied the exopolysaccharide produced by Cobetia marina DSMZ 4741, a marine bacterium isolated from coastal seawater. This strain is able to produce a polysaccharide in presence of carbon sources as glucose, mannitol and alginate. The maximum production occurs in aerobic condition, during the end of the exponential phase. The polymer is a non-viscous, acidic heteropolysaccharide of 270kDa constituted of a repeating unit of: This kind of chemical structure is generally related to K-antigen polysaccharide of pathogenic Escherichia coli strains. This is the first time this type of EPS is described from a marine bacterium. Moreover the polysaccharide exhibits a pyruvate substitution on its 3-deoxy-d-manno-oct-2-ulosonic acid (KDO) residue never encountered before. The discovery of such an unexpected EPS with high biotechnological potential is a new incentive for a better exploration of bioactive marine resources.
K-antigen, Kdo, Bacterial exopolysaccharides, Cobetia marina, Marine polysaccharide
Structure type: polymer chemical repeating unit
Location inside paper: p.354, table 2, K74
Trivial name: K74 antigen
Compound class: K-antigen
Contained glycoepitopes: IEDB_149136
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, sugar analysis, TLC, acid hydrolysis, HPAEC, GC, FTIR, NMR-1D, HPLC, UV, PAGE, reduction with NaBD4
Related record ID(s): 11039, 11185, 11186, 11187, 11188, 11189, 11190, 11192
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G34653TI, GlycomeDB:
5730
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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