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Taxonomic group: bacteria / Firmicutes (Phylum: Firmicutes)
Host organism: Homo sapiens
Associated disease: meningitis [ICD11: 1D01

]; neonatal septicemia [ICD11: KA60

, Life stage: neonatal]; infection due to Streptococcus [ICD11: XN3NM

]

The structure was elucidated in this paper
Publication DOI: 10.1139/v89-136
Journal NLM ID: 0372705
Publisher: National Research Council of Canada Canada
Institutions: Channing Laboratory, Department of Medicine, Brigham and Women's Hospital and Division of Infectious Diseases, Beth Israel Hospital, Harvard Medical School, Boston, MA 02115, U.S.A.

The native polysaccharide antigen isolated from type IV group B Streptococcus contains D-galactose, D-glucose, 2-acetamido-2-deoxy-D-glucose, and sialic acid in the molar ratio 2:2:1:1 and is composed of the following repeating unit: [formula: see text]. The structural analysis of this antigen was based on results obtained from methylation analysis, partial hydrolysis, nitrous acid deamination, and nuclear magnetic resonance spectroscopic techniques. Keywords: capsular polysaccharide, high resolution NMR spectroscopy, group B Streptococcus, polysaccharide structure, polysaccharide degradation.

capsular polysaccharide, group B Streptococcus, polysaccharide structure, polysaccharide degradation, high resolution NMR spectroscopy

Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 880, fig. 2
Compound class: CPS
Contained glycoepitopes: IEDB_130646,IEDB_135813,IEDB_136044,IEDB_136794,IEDB_137340,IEDB_137472,IEDB_140108,IEDB_140122,IEDB_141794,IEDB_141807,IEDB_142487,IEDB_142488,IEDB_144998,IEDB_146100,IEDB_146664,IEDB_149174,IEDB_150933,IEDB_151531,IEDB_153529,IEDB_158533,IEDB_190606,IEDB_423120,IEDB_983931,SB_115,SB_116,SB_131,SB_165,SB_166,SB_170,SB_171,SB_172,SB_187,SB_192,SB_195,SB_30,SB_39,SB_6,SB_68,SB_7,SB_84,SB_88

Methods: 13C NMR, 1H NMR, methylation, GLC-MS, gel filtration, NMR-2D, sugar analysis, GLC, de-N-acetylation/deamination, ion-exchange chromatography
Comments, role: Type IV GBS strain 3139

Related record ID(s): 10211
NCBI Taxonomy refs (TaxIDs): 1319
Reference(s) to other database(s): GTC:G45191WA, GlycomeDB:4639
Show glycosyltransferases

NMR conditions: in D2O at 310 K [as TSV]

1H NMR data:
Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4	aDGlcp	4.967	?	?	?	?	?
4,6,4,3,5	Ac
4,6,4,3	aXNeup
4,6,4	bDGalp	4.547	?	?	?	?	?
4,6,2	Ac
4,6	bDGlcpN	4.611	?	?	?	?	?
4	bDGalp	4.480	?	?	?	?	?
	bDGlcp	4.547	?	?	?	?	?

¹H NMR data:

Linkage	Residue	H1	H2	H3	H4	H5	H6
4,4	aDGlcp	4.967	?	?	?	?	?
4,6,4,3,5	Ac
4,6,4,3	aXNeup
4,6,4	bDGalp	4.547	?	?	?	?	?
4,6,2	Ac
4,6	bDGlcpN	4.611	?	?	?	?	?
4	bDGalp	4.480	?	?	?	?	?
	bDGlcp	4.547	?	?	?	?	?

¹³C NMR data: present in publication

There is only one chemically distinct structure:

*O[C@@H]1[C@@H](CO)O[C@H](O[C@H]2[C@@H](CO[C@@H]3O[C@H](CO)[C@@H](O[C@@H]4O[C@H](CO)[C@H](O)[C@H](O[C@]5(C(=O)O)C[C@H](O)[C@@H](NC(C)=O)[C@H]([C@H](O)[C@H](O)CO)O5)[C@H]4O)[C@H](O)[C@H]3NC(C)=O)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](*)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize