Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Sus scrofa
Associated disease: pleuropneumonia [ICD11:
CA40 
, ICD11:
CA40.Y ]
The structure was elucidated in this paperPublication DOI: 10.1139/v91-035Journal NLM ID: 0372705Publisher: National Research Council of Canada Canada
Institutions: Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ontario
The structure of the O-antigen of Actinobacillus pleuropneumoniae serotype 12 was determined by 1D and 2D NMR spectroscopic methods, methylation analysis, and partial hydrolytic degradation. The O-polysaccharide was shown to consist of a trisaccharide repeating unit having the structure [formula in text].
Lipopolysaccharide, antigen, polysaccharide, Actinobacillus pleuropneumoniae
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p. 222
Trivial name: galactan
Compound class: EPS, O-polysaccharide, O-antigen, cell wall polysaccharide
Contained glycoepitopes: IEDB_136095,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_149176,IEDB_151528,IEDB_190606,IEDB_885812,SB_7
Methods: 13C NMR, 1H NMR, methylation, GLC-MS, gel filtration, NMR-2D, sugar analysis, TLC, HF treatment
Related record ID(s): 630, 631, 108564
NCBI Taxonomy refs (TaxIDs): 754349Reference(s) to other database(s): GTC:G51971WH, GlycomeDB:
3478
Show glycosyltransferases
NMR conditions: in D2O at 300 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
6,6 aDGalp 99.3 69.0 70.2 70.0 71.8 61.9
6 bDGalf 108.8 81.7 77.5 82.8 74.4 68.5
bDGalf 107.9 82.1 77.5 83.4 70.3 70.0
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
6,6 aDGalp 4.99 3.83 3.87 3.98 3.97 3.76-3.76
6 bDGalf 5.04 4.14 4.13 4.11 4.19 3.70-3.98
bDGalf 5.28 4.13 4.04 4.05 3.97 3.63-3.87
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
6,6 aDGalp 99.3/4.99 69.0/3.83 70.2/3.87 70.0/3.98 71.8/3.97 61.9/3.76-3.76
6 bDGalf 108.8/5.04 81.7/4.14 77.5/4.13 82.8/4.11 74.4/4.19 68.5/3.70-3.98
bDGalf 107.9/5.28 82.1/4.13 77.5/4.04 83.4/4.05 70.3/3.97 70.0/3.63-3.87
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 6,6 | aDGalp | 4.99 | 3.83 | 3.87 | 3.98 | 3.97 | 3.76
3.76 |
| 6 | bDGalf | 5.04 | 4.14 | 4.13 | 4.11 | 4.19 | 3.70
3.98 |
| | bDGalf | 5.28 | 4.13 | 4.04 | 4.05 | 3.97 | 3.63
3.87 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 6,6 | aDGalp | 99.3 | 69.0 | 70.2 | 70.0 | 71.8 | 61.9 |
| 6 | bDGalf | 108.8 | 81.7 | 77.5 | 82.8 | 74.4 | 68.5 |
| | bDGalf | 107.9 | 82.1 | 77.5 | 83.4 | 70.3 | 70.0 |
|
There is only one chemically distinct structure:
Found 
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(previously named: Haemophilus pleuropneumoniae 12 (NRCC 4267))