Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: hemorrhagic colitis (HC) [ICD11:
1A40.0 
];
hemolytic-uremic syndrome (HUS) [ICD11:
3A21.2 ];
infection due to Escherichia coli [ICD11:
XN6P4 ]
The structure was elucidated in this paperNCBI PubMed ID: 29348238Publication DOI: 10.1126/science.aao4096Journal NLM ID: 0404511Publisher: Washington, DC: American Association for the Advancement of Science
Correspondence: cegelski
stanford.edu; regine.hengge
hu-berlin.de
Institutions: Department of Chemistry, Stanford University, Stanford, CA 94305, USA, Institute of Biology, Microbiology, Humboldt-Universitat zu Berlin, 10115 Berlin, Germany, Department of Chemistry and Molecular Biology, University of Gothenburg, 41296 Gothenburg, Sweden
Cellulose is a major contributor to the chemical and mechanical properties of plants and assumes structural roles in bacterial communities termed biofilms. We find that Escherichia coli produces chemically modified cellulose that is required for extracellular matrix assembly and biofilm architecture. Solid-state nuclear magnetic resonance spectroscopy of the intact and insoluble material elucidates the zwitterionic phosphoethanolamine modification that had evaded detection by conventional methods. Installation of the phosphoethanolamine group requires BcsG, a proposed phosphoethanolamine transferase, with biofilm-promoting cyclic diguanylate monophosphate input through a BcsE-BcsF-BcsG transmembrane signaling pathway. The bcsEFG operon is present in many bacteria, including Salmonella species, that also produce the modified cellulose. The discovery of phosphoethanolamine cellulose and the genetic and molecular basis for its production offers opportunities to modulate its production in bacteria and inspires efforts to biosynthetically engineer alternatively modified cellulosic materials
biosynthesis, genetic, Escherichia coli, Biofilm, cellulose, phosphoethanolamine transferase, transmembrane
Structure type: fragment of a bigger structure
Location inside paper: fig.1A
Trivial name: pEtN cellulose
Compound class: EPS
Contained glycoepitopes: IEDB_120354,IEDB_123890,IEDB_142488,IEDB_146664,IEDB_241118,IEDB_983931,SB_192
Methods: genetic methods, STD NMR, LC-ESI-MS, REDOR NMR
NCBI Taxonomy refs (TaxIDs): 316407,
83333,
1095706Reference(s) to other database(s): GTC:G58330YH
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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