Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 27196310Publication DOI: 10.1016/j.carres.2016.04.012Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: fenglu63
nankai.edu.cn
Institutions: N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, China, The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Tianjin, China
Glycosyltransferases (GTs) catalyze the formation of regio- and stereo-specific glycosidic linkages between specific sugar donors and recipients. In this study, the function of the gene wclR from the Escherichia coli O3 O-antigen gene cluster that encodes an ? 1, 3-galactosyltransferase (GalT) that acts on the linkage Gal α1,3-GlcNAc was biochemically characterized. WclR was expressed in E.coli BL21 (DE3), and the enzymatic product was identified by liquid chromatography-mass spectrometry (LC-MS), collision-induced dissociation electrospray ionization ion trap multiple tandem MS (CID-ESI-IT-MS(n)) and galactosidase digestion, using UDP-Gal as the donor substrate and the synthetic acceptor substrate GlcNAc-PP-De (decyl diphosphate N-acetylglucosamine). The physiochemical properties and the substrate specificity of WclR were investigated. WclR is the first bacterial GalT characterized that acts on the linkage Gal α1,3-GlcNAc. This study enhanced our knowledge of the diversified functions of GTs and provided a novel enzyme source for possible pharmaceutical application.
Escherichia coli, mass spectrometry, galactosyltransferase, O-antigen gene cluster, WclR
Structure type: suggested polymer biological repeating unit
Location inside paper: p.37, fig.1
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_135813,IEDB_136906,IEDB_137340,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_190606,IEDB_983931,SB_173,SB_192,SB_7
Methods: PCR, Western blotting, genetic methods, biochemical methods, enzyme assay, LC-ESI-MS, CID-ESI-IT-MS
Comments, role: biological repeat frame was based on [PMID:27196310]
Related record ID(s): 10304, 20634, 22724
NCBI Taxonomy refs (TaxIDs): 2234097Reference(s) to other database(s): GTC:G44739ZN, GlycomeDB:
28095
Show glycosyltransferases
There is only one chemically distinct structure:
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