Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Host organism: Homo sapiens
Associated disease: cystic fibrosis (CF) [ICD11:
CA25 
]
The structure was elucidated in this paperNCBI PubMed ID: 26586192Publication DOI: 10.1099/mic.0.000214Journal NLM ID: 0376646Publisher: Washington, DC: Kluwer Academic/Plenum Publishers
Correspondence: pcescutti
units.it (Paola Cescutti)
Institutions: Department of Life Sciences, University of Trieste, via L. Giorgieri 1, Bdg C11, 34127Trieste, Italy
Bacteria usually grow forming biofilms, which are communities of cells embedded in a self-produced dynamic polymeric matrix, characterized by a complex three-dimensional structure. The matrix holds cells together and above a surface, and eventually releases them, resulting in colonization of other surfaces. Although exopolysaccharides (EPOLs) are important components of the matrix, determination of their structure is usually performed on samples produced in non-biofilm conditions, or indirectly through genetic studies. Among the Burkholderia cepacia complex species, Burkholderia cenocepacia is an important pathogen in cystic fibrosis (CF) patients and is generally more aggressive than other species. In the present investigation, B. cenocepacia strain BTS2, a CF isolate, was grown in biofilm mode on glass slides and cellulose membranes, using five growth media, one of which mimics the nutritional content of CF sputum. The structure of the matrix EPOLs was determined by 1H-NMR spectroscopy, while visualization of the biofilms on glass slides was obtained by means of confocal laser microscopy, phase-contrast microscopy and atomic force microscopy. The results confirmed that the type of EPOLs biosynthesized depends both on the medium used and on the type of support, and showed that mucoid conditions do not always lead to significant biofilm production, while bacteria in a non-mucoid state can still form biofilm containing EPOLs.
exopolysaccharides, biofilms, atomic force microscopy, Burkholderia cepacia complex, Burkholderia cenocepacia, Host-microbe Interaction
Structure type: fragment of a bigger structure
Location inside paper: p.289, table 3
Trivial name: α-D-glucan, glycogen, amylose-like α-D-glucan
Compound class: EPS, cell wall polysaccharide
Contained glycoepitopes: IEDB_140629,IEDB_142488,IEDB_144998,IEDB_146664,IEDB_420417,IEDB_420418,IEDB_420421,IEDB_857742,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, methylation, NMR-2D, sugar analysis, ESI-MS, acid hydrolysis, GLC, permethylation, reduction with NaBD4, confocal scanning laser microscopy, colorimetric assays, protease treatment, morphological analysis
Comments, role: Biofilm formed on glass slides contained a glycogen
Related record ID(s): 11690
NCBI Taxonomy refs (TaxIDs): 95486Reference(s) to other database(s): GTC:G05740LL
Show glycosyltransferases
NMR conditions: in D2O at 323 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
aDGlcp 102.51 74.39 76.12 79.96 74.06 63.37
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
aDGlcp 5.37 3.62 3.95 3.64 3.83 3.81-3.86
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
aDGlcp 102.51/5.37 74.39/3.62 76.12/3.95 79.96/3.64 74.06/3.83 63.37/3.81-3.86
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| | aDGlcp | 5.37 | 3.62 | 3.95 | 3.64 | 3.83 | 3.81
3.86 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| | aDGlcp | 102.51 | 74.39 | 76.12 | 79.96 | 74.06 | 63.37 |
|
There is only one chemically distinct structure:
Found 
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