The structure was elucidated in this paper NCBI PubMed ID:3896488 Publication DOI:10.1016/0008-6215(85)90025-4 Journal NLM ID:0043535 Publisher: Elsevier Institutions: Max-Planck-Institut für Immunbiologie, D-7800 Freiburg, West Germany
The structure of the K54-antigenic polysaccharide (K54 antigen) of Escherichia coli O6:K54:H10 was elucidated by determination of the composition, 1H- and 13C-n.m.r. spectroscopy, periodate oxidation, and a study of the oligosaccharides obtained by partial hydrolysis with acid. The K54 polysaccharide consists of →3)-β-D-glucosyluronic acid-(1→3)-α-L-rhamnosyl-(1→ repeating-units. Of the glucuronic acid residues, approximately 85% are substituted in the ratio 9:1 with L-threonine and L-serine amidically linked to the carboxyl group. The K54 polysaccharide has a molecular weight of approximately 160,000, corresponding to approximately 380 repeating-units.
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