Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
Associated disease: infection due to Bacillus subtilis [ICD11:
XM4SG9 
]
The structure was elucidated in this paperNCBI PubMed ID: 30553388Publication DOI: 10.1016/j.carbpol.2018.11.062Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: G. Wang <guodong201
csu.edu.cn>; K. Chen <ksc313
126.com>
Institutions: School of Life Science, Shandong University, Qingdao 266000, China, National Glycoengineering Rechearch Center, Shandong University, Qingdao 266000, China, Anhui Provincial Engineering Research Center for Polysaccharide Drugs, Anhui Province Key Laboratory of Active Biological Macromolecules, Drug Research & Development Center, School of Pharmacy, Wannan Medical College, Wuhu, China
In this study, a polysaccharide, BP, was produced by Bacillus subtilis using burdock oligofructose as a carbon source. The polysaccharide BP was isolated from burdock fermented through B. subtilis by hot water extraction, ethanol precipitation, anion exchange and gel filtration chromatography. The molecular weight of BP was determined to be 1.533 x 107 g/mol, and is composed of mannose, rhamnose, glucose, galactose, and arabinose in the ratio of 1:0.12:17.16:0.69:1.10. Furthermore, BP is made up of a backbone structure of (1→6)-linked α-d-glucopyranosyl residues substituted at the O-3 position with α-d-glucopyranosyl branches. In vitro, BP inhibited A549 cell proliferation and increased reactive oxygen species production, thus, promoting the apoptosis of cells. Moreover, BP up-regulated the expression of caspase-3 and the corresponding protein, indicating that it activates the caspase-3 pathway. These findings suggest that the potential of BP as a supplement or drug to treat cancer.
polysaccharide structure, Bacillus subtilis, apoptosis, Fermented burdock, Lung cancer
Structure type: structural motif or average structure ; 15330000 g/mol
Location inside paper: p.816, fig.6
Trivial name: BP neutral polysaccharide
Contained glycoepitopes: IEDB_142488,IEDB_144998,IEDB_146664,IEDB_158538,IEDB_2346541,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, GC-MS, anion-exchange chromatography, Western blotting, FTIR, composition analysis, HPLC, HPSEC-MALLS, UV, statistical analysis, antitumor activity assay, qRT-PCR
Biological activity: The treatment with BP (50 and 100 μg/mL for 24 h), apoptosis of A549 cells increased to 14.85% (p < 0.05) and 27.60% (p < 0.01).
NCBI Taxonomy refs (TaxIDs): 1423Reference(s) to other database(s): GTC:G14569WK
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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